Introduction This paper sought to scrutinize the role of microRNA-32 (miR-32) on the growth and migration as well as on the expression of metastatic genes in PC3 cells of prostate cancer in vitro. Methods Subsequent transfection of cells with miR-32 mimics, miR-32 inhibitor, negative control (NC), cell proliferation using MTT, and apoptosis by ELISA were performed. Furthermore, qRT-PCR was directed to measure the expression levels of matrix metalloproteinase 2 (MMP2) and vascular endothelial growth factors (VEGF) as metastatic and angiogenesis genes in the progression of PC3. Results miR-32 was overexpressed in PC3 cells compared to normal cells (P<0.001). Down-regulation of miR-32 obstructs in vitro proliferation and migration while intensifying the apoptosis rate in PC3 cells. Also, we found that miR-32 negatively modulates the expression of VEGF and MMP2 in PC3 cells. Conclusion These results indicate that the suppression of miR-32 might offer an auxiliary treatment procedure for addressing the invasion, progression, and metastasis in PCa patients by improving cell apoptosis.
One hundred wound swab samples were collected from wound patients who visited in the Teaching Hospital wound unit in Hillah, Babylon province, Iraq. S. aureus was identified morphologically and biochemically. Total of 30 (30%) from total samples exhibited positive culture for S. aureus. Out of 30 S. aureus, 8 (26.6%) isolates were MRSA, Antibiotic susceptibility was tested for 8 antibiotics for MRSA that appeared sensitive toward Tetracycline, Rifampin and Ciprofloxacin (62.5%) but (37.5%) were resistant. For PenicillinG and Cefoxitin, all isolates were resistant (100%). For Clindamycin, (25%) of isolates were resistant but (75%) were sensitive. About (62.5%) were resistant to Erythromycin but (37.5%) were sensitive. Isolates showed resistance to Gentamycin in 50% and 50% sensitive. Anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against S. aureus displays excessive widespread spectrum antibacterial action against established bacteria with increase zone of inhibition diameter that is proportional with the increase in nanoparticle concentration. The (MIC of CuO NPs ranged from 75-150μg/ml and the MBC ranged from 150-300μg/ml. CuO NPs is recommended as an efficient anti-MRSA alternative.
The evaluation of in-vitro anti-Candida activity was done for Ricinus communism leaves extracts which are yielded by the extraction with different organic solvents (methanol, chloroform, and a combination of these solvents((1:1; v/v); methanol/chloroform)) against four Candida species(tropicalis, kefyr, glabrata, and albicans) by agar well diffusion method. In the present study, the results showed that all tested extracts possessed anti-Candida activity against all examined strains, but the leaves extracted by a combination of methanol and chloroform exhibited the best anti-Candida potentiality when compared with methanol or chloroform solvent which is separately used in the extraction process, in addition to that the anti-Candida activity of all leaves extracts was varied depending on the Candida species susceptibility and the concentration of the extract which used in every treatment, in addition to that the minimum inhibitory concentrations data of the combined solvents extract were (13, 18, 22, and 24% for Candida glabrata, tropicalis, kefyr, and albicans respectively.
Background: Cardiorenal syndrome, a term used to describe a variety of heart and kidney illnesses, is characterized by the dysfunction of one organ resulting to the dysfunction of the other. Objective:The aim of the current study to evaluate some biochemical indicators, linking hematologic analysis and kidney function test with interleukin-1 receptor family biomarker (ST2), and investigating the early diagnosis and prediction of chronic renal syndrome. Patients and methods: A total of 60 cases were recruited; comprising 20 controls and 40 patients with chronic kidney disease and cardiovascular disease. The patients were aged between 25 and 65 years old. Levels of ST2 were measured in both the case and the control groups. Result: In comparison with the control group, patients with cardiovascular diseases had higher concentrations of haematological features than do patients with chronic kidney disease. Also, when compared to controls, those with chronic kidney disease had statistically significant (P<0.05) increase in their serum levels of urea and creatinine. When compared to other groups, the levels of the biomarker ST2 were significantly higher in chronic kidney disease patients. Conclusions: Adults with chronic kidney disease had higher circulating ST2 levels and a higher mortality rate. RBC negatively affects ST2 levels as a result there is a link between ST2 levels and the risk of anaemia in chronic kidney disease patients.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.