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SARS-CoV-2 is a virus that causes a serious lifethreatening disease known as COVID-19. Patients with COVID-19 can develop acute respiratory distress syndrome (ARDS) and multiorgan failure requiring safe and effective therapy. The human Wharton's Jelly Mesenchymal Stem Cells (hWJMSCs) can act as an anti-inflammatory and regenerative properties due to their secretion such as cytokine, growth factor, antimicrobial, and chemokine. A natural way of producing the secreted proteins of a cell (secretome) is by culturing mesenchymal stem cells (MSCs) under starvation or serum deprivation condition. This research was performed to measure the level of Fibroblast Growth Factor-7 (FGF-7), antibacterial protein LL-37 (LL-37), interleukin 1 Receptor Antagonist (IL-1ra), respectively, in the secretome of hWJMSCs. The secretome levels at: non-starvation, 24-, 48-, and 72-hours starvation periods were measured using ELISA method. The hWJMSCs secreted levels of FGF-7, LL-37, and IL-1ra were 46.74 -72.13 pg/mL, 1.70 -4.14 ng/mL, 1.35 -8.32 pg/mL, respectively. The longer the starvation period of cells, the higher the protein levels produced. The hWJMSCs secrete FGF-7, LL-37, IL-1ra, longer starvation periods of cells will increase secretome levels. The hWJMSCs could potentially serve as adjuvant therapy for COVID-19.

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Jejas hati imbas obat ditandai dengan peningkatan kadar enzim hati dan kerusakan jaringan hati. Jejas hati imbas obat sering disebabkan oleh konsumsi beberapa obat seperti parasetamol dan rifampisin. Jejas hati imbas obat dapat dibagi menjadi jejas hati imbas obat tipe intrinsik dan jejas hati imbas obat tipe idiosinkratik. Penatalaksanaan jejas hati imbas obat adalah dengan menghentikan konsumsi obat terkait. Salah satu obat herbal asli Indonesia adalah tanaman sambiloto (Andrographis paniculata) yang memiliki banyak manfaat salah satunya sebagai hepatoprotektor dan agen antiinflamasi. Pemberian sambiloto (Andrographis paniculata) pada mencit maupun tikus yang mengalami jejas hati imbas obat menyebabkan penurunan kadar enzim hati di dalam darah dan memperbaiki gambaran histologis hati sehingga sambiloto dapat menjadi salah satu terapi adjuvant untuk mengatasi jejas hati imbas obat.

Background and Objective: Lactobacillus acidophilus has been widely used for the management of gastrointestinal carcinoma owing to its immunomodulation effect; however, the role of L. acidophilus and its specific mechanism of action in the stomach is not fully comprehended. The present study evaluated the expression profile of MUC-1, GAL-3, IL -1β, and IL-17 in the L. acidophilus treated mice stomach. Methods: The study was conducted utilizing three groups of mice, 6 mice for each group, administered with different doses of L. acidophilus and a control group treated with normal saline. The results were analyzed with the Mann-Whitney Test. Results: The results demonstrated that L. acidophilus elevated IL-1β insignificantly and inhibited the expression of IL-17. The MUC-1 expression is influenced by L. acidophilus and inversely proportional to GAL-3 expression. Conclusion: Lactobacillus acidophilus plays a prominent role against inflammatory responses and has a potential in the treatment of gastrointestinal cancer.

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