Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Widowati, Wahyu; Wargasetia, TeresaLiliana; Rahardja, Fanny; Gunanegara, RimontaF; Priyandoko, Didik; Gondokesumo, MariscaEvalina; Afifah, Ervi; Wijayanti, CahyaningRiski; Rizal, Rizal

doi:10.4103/2221-1691.350182

Cited by 5 publications

(8 citation statements)

References 26 publications

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“…In our previous study, hWJMSCs were found to decrease lung inflammation in ARDS rats through decreasing IL-18 and IL-1β, and consequently suppressing the NFκB cascade. 18 …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a previous study has indicated that hWJMSCs treatment in animals lungs upregulates FGF-7, thereby inhibiting apoptosis. 1 , 18 , 33 …”

Section: Discussionmentioning

confidence: 99%

“…Inflamed lung cells are a feasible ARDS model. 18 Therefore, this study evaluated the effects of human Wharton's jelly MSCs (hWJMSCs) in suppressing inflammation in lipopolysaccharide (LPS)-induced rat lung cells, as an ARDS cell model. ACE-2, CXCL-9, NFκB, and RAGE gene expression, as well as CRP, TNF-α, and IL-12 levels, and apoptosis percentages were measured.…”

Section: Introductionmentioning

confidence: 99%

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hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model

Widowati

Wargasetia

Rahardja

et al. 2023

Journal of Taibah University Medical Sciences

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“…In our previous study, hWJMSCs were found to decrease lung inflammation in ARDS rats through decreasing IL-18 and IL-1β, and consequently suppressing the NFκB cascade. 18 …”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a previous study has indicated that hWJMSCs treatment in animals lungs upregulates FGF-7, thereby inhibiting apoptosis. 1 , 18 , 33 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model

Widowati

Wargasetia

Rahardja

et al. 2023

Journal of Taibah University Medical Sciences

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“…The lungs were then snap-frozen in liquid nitrogen and stored at -80°C until used. Lungs were stored and dissected for histopathological analysis and immunohisto-chemistry [18][19][20].…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…The sample analysis was according to the protocol of manufacture with a modified method. The absorbance was read triplicate at 450 nm (Multiskan GO, Thermo Scientific) [18,20].…”

Section: Quantification Of Lung Il-18 Il-12 and Serum Il-1β Tnf-αmentioning

confidence: 99%

Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model

PRIYANDOKO,

WIDOWATI,

NOVIANTO

et al. 2024

jrp

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COVID-19 infection is always accompanied by a cytokine storm that causes acute respiratory distress syndrome (ARDS). The reduction of pro-inflammatory cytokines is expected to be used for treating COVID-19 patients. Guava (Psidium guajava L.) was believed to have properties as an antioxidant and anti-inflammatory. This study aims to determine the effect of guava extract (PGE) on the ARDS rats model so that it can be used as a PGE treatment for the effects caused by ARDS. This study was conducted on 25 Sprague Dawley rats model in 5 treatment groups. Lipopolysaccharides (LPS) induction was carried out to create ARDS rats model. Determination of pro-inflammatory cytokines in lung Interleukin-18 (IL-18), IL-12 and serum IL-1β, Tumour Necrosis Factor alpha (TNF-α) was performed by ELISA, NOD-, LRR-and Pyrin domain-containing protein 3 (NRLP3) expressions were determined by the quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) method and IL-6, Nuclear factor kappa B (NF-κB) were analysed by immunohisto-chemistry assay. The results showed that PGE contained phenolic compound, daidzin and genistin. The IL-18, IL-12, Il-1β, TNF-α, IL-6, NF-kB and NRLP3 in ARDS model rats were decreased by PGE treatment. In conclusion, PGE can be used as a therapy for treating ARDS by decreasing pro-inflammatory marker.

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Relationship between neonatal respiratory distress syndrome pulmonary ultrasonography and respiratory distress score, oxygenation index, and chest radiography grading

Yang,

Gao,

Lei

et al. 2024

World J Clin Cases

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BACKGROUND Accurate condition assessment is critical for improving the prognosis of neonatal respiratory distress syndrome (RDS), but current assessment methods for RDS pose a cumulative risk of harm to neonates. Thus, a less harmful method for assessing the health of neonates with RDS is needed. AIM To analyze the relationships between pulmonary ultrasonography and respiratory distress scores, oxygenation index, and chest X-ray grade of neonatal RDS to identify predictors of neonatal RDS severity. METHODS This retrospective study analyzed the medical information of 73 neonates with RDS admitted to the neonatal intensive care unit of Liupanshui Maternal and Child Care Service Center between April and December 2022. The pulmonary ultrasonography score, respiratory distress score, oxygenation index, and chest X-ray grade of each newborn before and after treatment were collected. Spearman correlation analysis was performed to determine the relationships among these values and neonatal RDS severity. RESULTS The pulmonary ultrasonography score, respiratory distress score, oxygenation index, and chest X-ray RDS grade of the neonates were significantly lower after treatment than before treatment (P < 0.05). Spearman correlation analysis showed that before and after treatment, the pulmonary ultrasonography score of neonates with RDS was positively correlated with the respiratory distress score, oxygenation index, and chest X-ray grade (ρ = 0.429–0.859, P < 0.05). Receiver operating characteristic curve analysis indicated that pulmonary ultrasonography screening effectively predicted the severity of neonatal RDS (area under the curve = 0.805–1.000, P < 0.05). CONCLUSION The pulmonary ultrasonography score was significantly associated with the neonatal RDS score, oxygenation index, and chest X-ray grade. The pulmonary ultrasonography score was an effective predictor of neonatal RDS severity.

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Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model

Cited by 5 publications

References 26 publications

hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model

hWJMSCs inhibit inflammation and apoptosis in an ARDS cell model

Guava (Psidium guajava L.) extract inhibits cytokine storm in acute respiratory distress syndrome rats model

Relationship between neonatal respiratory distress syndrome pulmonary ultrasonography and respiratory distress score, oxygenation index, and chest radiography grading

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