Exosomes are considered as potential biomarkers that can reflect information from their parent cell-associated cancer microenvironment.

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A platinum nanoparticle‐modified chiral cross‐linked cyclodextrin MOF (Pt‐CLMOF) was synthesized. Then, a chiral electrochemical sensor was constructed using the Pt‐CLMOF nanomaterials for the recognition of methionine (Met) enantiomers and the chiral recognition mechanism was revealed by density functional theory calculations. The recognition effect of Pt‐CLMOF modified electrode (Pt‐CLMOF/GCE) on Met was studied by differential pulse voltammetry. The enantioselectivity coefficient (IL/ID) was 1.98. There is an acceptable linear relationship between peak current and Met enantiomer concentration in the 2–200 μM range, the detection limits of L–Met and D‐Met were 0.33 and 0.60 μM which were obtained by this electrochemical sensor, respectively. The synthesized materials were successfully applied to the analysis of Met enantiomers, indicating that Pt‐CLMOF materials have good application potentiality.

Short‐chain and medium‐chain fatty acids have plentiful biological functions, which play a crucial role in the diagnosis and therapy of many diseases. Herein, a new method for simultaneous quantifying 17 short‐chain and medium‐chain fatty acids with high‐performance liquid chromatography coupled with an ultraviolet detector was developed and the pre‐column derivatization by indole‐3‐acetic acid hydrazide was performed to improve the separation and detection. The conditions of the derivatization reaction were systematically investigated. Subsequently, the method was validated and the results showed a satisfactory linearity (linear regression coefficients > 0.9969), the limit of detection (4.0×10−3–1.9×10−2 μmol/L), precision (0.9%–7.3% for intra‐day and 2.0%–9.8% for inter‐day), recovery (90.0%–109.1% with relative standard deviation <7.7%) and stability (0.1%–3.3% for standard solution and 0.2%–3.9% for fecal sample). Finally, the established method was successfully applied to quantify short‐chain and medium‐chain fatty acids in the feces of healthy control and diabetic rats. Eleven kinds of short‐chain and medium‐chain fatty acids were detected and six of them showed a significant difference between the control group and the model group.