The perforating and deep lymphatic vessels in the knee region has been presented and discussed. The information advances our anatomical knowledge and the results will benefit clinical management.
Maoji Jiu (MJ) is a kind of medicinal wine that has been widely used by Chinese people for many years to nourish and promote blood circulation. The purpose of this study was to investigate the hematopoietic effect of MJ on the metabolism of blood deficient rats and to explore the underlying hematopoietic regulation mechanisms. Blood deficiency model rats were induced by subcutaneous injection of N-acetylphenylhydrazine (APH) and intraperitoneal injection of cyclophosphamide (CTX). The plasma metabolic fingerprints of blood deficiency model rats with and without MJ treatment were obtained by using metabonomics based on ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC–QTOF/MS). Orthogonal partial least squares-discriminant analysis (OPLS–DA) was used to evaluate the hematopoietic effect of MJ and identify potential biomarkers in the plasma of blood deficiency model rats. The levels of white blood cells (WBC), red blood cells (RBC) and hemoglobin (HGB) and the activity of antioxidant capacity showed a recovery trend to the control group after MJ treatment, while the dose of 10 mL/kg showed the best effect. In this study, thirteen potential biomarkers were identified, which were mainly related to seven metabolic pathways, including linoleic acid metabolism, d-glutamine and d-glutamate metabolism, alanine, aspartate and glutamate metabolism, tryptophan metabolism, pyrimidine metabolism, porphyrin and chlorophyll metabolism and arginine biosynthesis. Metabolomics was applied frequently to reflect the physiological and metabolic state of organisms comprehensively, indicating that the rapid plasma metabonomics may be a potentially powerful tool to reveal the efficacy and enriching blood mechanism of MJ.
There is no authoritative identification of the attributes of the haemolymph node since Gibbes first described it in 1884. Early literatures showed that it was found near the kidney in human and animals with the characteristics of numerous erythrocytes in sinuses. Subsequent studies were mainly focused in the field of anatomy and histology, such as the source, distribution and quantity of erythrocytes in sinuses. Recent articles mentioned that the emergence of the haemolymph node was related to immunity, but there was no strong evidence to support the hypothesis. Therefore it is still uncertain whether the haemolymph node is an organ of anatomy, histology or immunology. It has been found that the development of the haemolymph node can be elicited in the parathymic area by stimuli such as Escherichia coli, allogeneic breast cancer cells and renal tissue that were injected/transplanted into the tail of rats in our pilot studies. In this study, the model of the haemolymph node was established by transferring the allogeneic renal tissue in the rat. Intrasinusoidal erythrocytes of the node were the component for producing a red macroscopic appearance, while macrophage-erythrocyte-lymphocyte rosettes were the major immunomorphological changes, which reflects the immune activity against the invasion of the allogeneic tissue within the node. Therefore the haemolymph node is an immunomorphological organ.
Maoji Jiu (MJ), a medicinal wine, has been used commonly by the Chinese to enrich and nourish the blood. In this study, the aim is to examine the hematopoietic function of MJ and investigate its hematopoietic regulation mechanism. Thirty-six female Sprague-Dawley rats (200 ± 20 g) were randomly divided into six groups with six rats in each group. The blood deficiency model was induced by injecting hypodermically with N-acetylphenylhydrazine (APH) and injecting intraperitoneally with cyclophosphamide (CTX), and treatment drugs were given by oral gavage twice a day for continuous 10 days from the start of the experiments. The administration of MJ improved the levels of white blood cells (WBCs), red blood cells (RBCs), hemoglobin (HGB), and hematocrit (HCT) in the blood deficiency model rats. Hematopoietic effect involves regulating the antioxidant activity in the liver and the levels of Bcl-2, Bax, erythropoietin (EPO), transforming growth factor-beta-1 (TGF-β1), and macrophage colony-stimulating factor (M-CSF) mRNA in spleen tissues to enhance extramedullary hematopoiesis. This study suggests that MJ has a beneficial effect on blood deficiency model rats.
Objective: To determine morphological and ultrastructural details of the collecting lymph vessel, lumbar trunk and thoracic duct of the rat. Methods: Sixteen adult SD rats were utilized for the study. A small amount of 6% hydrogen peroxide was applied to find the lymph vessel in the dorsodistal hind-limb and sides of the abdominal aorta in the rat. Under a surgical microscope, the vessel was injected by Indian ink or a radiopaque mixture via a fine needle to locate sites of the collecting lymphatic vessel, lumbar trunk and thoracic duct. Vessels were then harvested for the histological and transmission electron microscopic examinations. Results: Diameters of the collecting lymph vessel, lumbar trunk and thoracic duct in the rat were diverse, small in the former and large in the latter. Containing multiple valves in the lumen, their walls were very thin and composed of three layers of tissue. Ultrastructures of the tunica intima and externa of these vessels were similar. A single layer of endothelium cells with a discontinuous basement membrane formed the tunica intima of the vessel. One or two discontinuous layers of smooth muscle cells was found in the tunica media of the collecting lymph vessel, one to three layers of smooth muscle cells in the lumbar trunk and three to five layers of smooth muscle cells in the thoracic duct. The tunica externa of the vessel was comprised by connective tissue (collagen fibrils) and fibroblasts. Conclusion: Morphological and ultrastructural details of the collecting lymph vessel, lymphatic trunk and duct in the rat have been demonstrated that may help for the purpose of further lymphatic vessels studies.
Background: Maoji Jiu (MJ), a medicinal wine, has been used commonly by Chinese to enrich and nourish the blood. In this study, the aim is to examine the hematopoietic function of MJ and investigate its anti-blood deficiency mechanism. Methods: 36 rats were randomly divided into six groups with six rats in each group. The blood deficiency model was induced by injecting with N-acetylphenylhydrazine (APH) and injected intraperitoneally with cyclophosphamide (CTX), and treatment drugs were given by oral gavage twice each day for continuous 10 days from the start of experiments. Results: The administration of MJ improved the levels of white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB) and hematocrit (HCT) in the blood deficiency model rats. Hematopoietic effect involves in regulating the antioxidant activity in the liver and the levels of Bcl-2, Bax, erythropoietin (EPO), transforming growth factor-beta-1 (TGF-β1) and macrophage colony-stimulating factor (M-CSF) mRNA in spleen tissues to enhance extramedullary hematopoiesis. Conclusion: This study suggests that MJ has a beneficial effect on blood deficiency model rats.
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