The etiology of mandibular prognathism has been attributed to various genetic inheritance patterns and some environmental factors. The variation in inheritance patterns can be partly due to the use of different statistical approaches in the respective studies. The objective of this study was to investigate the role of genetic influences in the etiology of this trait. We performed segregation analysis on 37 families of patients currently being treated for mandibular prognathism. Mandibular prognathism was treated as a qualitative trait, with cephalometric radiographs, dental models, and photographs used to verify diagnosis. Segregation analysis of a prognathic mandible in the entire dataset supported a transmissible Mendelian major effect, with a dominant mode of inheritance determined to be the most parsimonious.

The results of this study suggest that both autogenous bone graft and Bio-Oss played an important role in the amount of hard tissue fill and osseointegration occurring within marginal bone defects around implants.

The purpose of this study was to evaluate effects of titanium surfaces air-abraded with particles of Bioglass® 45S5 and three-ZnO and SrO doped compositions on the viability, adhesion and biofilm formation of Streptococcus mutans. A statistically significant decrease in the viability of S. mutans was observed for all titanium discs air-particle abraded with the BAGs (p<0.001). Also, a significant effect on diminishing biofilm formation on the titanium discs was seen for all BAGs (p<0.01). No differences were noticed in S. mutans adhesion on titanium surfaces treated with different glasses (p=0.964). Static SBF immersion experiments showed that after 2 and 48 h the BAG doped with 4 mol% ZnO demonstrated the highest Zn 2+ ion concentration released into SBF (0.2 mg L −1 ). 45S5 BAG demonstrated the highest statistically significant increase in the pH throughout the 120 min of static immersion (p<0.001). In conclusion, we showed that titanium alloy discs abraded with particles of the experimental compositions and 45S5 BAG had strong antimicrobial activity against S. mutans and they suppressed S. mutans biofilm formation. The antimicrobial activity of 45S5 BAG was attributed to high pH whereas for the Zn-containing BAGs antimicrobial activity was due to steady release of Zn 2+ into the interfacial solution.

Streptococcus mutans is able to form a high-affinity biofilm on material surfaces. S mutans has also been detected around infected implants. Bioactive glasses (BAGs) have been shown to possess antibacterial effects against S mutans and other microorganisms. This in vitro study was performed to investigate the influence of BAG air abrasion on S mutans biofilm on sandblasted and acid-etched titanium surfaces. Sandblasted and acid-etched commercially pure titanium discs were used as substrates for bacteria (n = 107). The discs were immersed in an S mutans solution and incubated for 21 hours to form an S mutans biofilm. Twenty colonized discs were subjected to air abrasion with Bioglass 45S5 (45S5 BAG), experimental zinc oxide containing BAG (Zn4 BAG), and inert glass. After the abrasion, the discs were incubated for 5 hours in an anaerobic chamber followed by an assessment of viable S mutans cells. Surface morphology was evaluation using scanning electron microscopy (n = 12). The thrombogenicity of the glass particle–abraded discs (n = 75) was evaluated spectrophotometrically using whole-blood clotting measurement at predetermined time points. Air abrasion with 45S5 and Zn4 BAG eradicated S mutans biofilm. Significantly fewer viable S mutans cells were found on discs abraded with the 45S5 or Zn4 BAGs compared with the inert glass (P &lt; .001). No significant differences were found in thrombogenicity since blood clotting was achieved for all substrates at 40 minutes. Air abrasion with BAG particles is effective in the eradication of S mutans biofilm from sandblasted and acid-etched titanium surfaces. Zn4 and 45S5 BAGs had similar biofilm-eradicating effects, but Zn4 BAG could be more tissue friendly. In addition, the steady release of zinc ions from Zn4 may enhance bone regeneration around the titanium implant and may thus have the potential to be used in the treatment of peri-implantitis. The use of either BAGs did not enhance the speed of blood coagulation.

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The aim of this study was to evaluate the hydrophilicity, surface free energy, and proliferation and viability of human osteoblast‐like MC3T3‐E1 cells on sandblasted and acid‐etched titanium surfaces after air‐abrasion with 45S5 bioactive glass, zinc‐containing bioactive glass, or inert glass. Sandblasted and acid‐etched titanium discs were subjected to air‐abrasion with 45S5 bioactive glass, experimental bioactive glass (Zn4), or inert glass. Water contact angles and surface free energy were evaluated. The surfaces were studied with preosteoblastic MC3T3‐E1 cells. Air‐abrasion with either type of glass significantly enhanced the hydrophilicity and surface free energy of the sandblasted and acid‐etched titanium discs. The MC3T3‐E1 cell number was higher for substrates air‐abraded with Zn4 bioactive glass and similar to that observed on borosilicate coverslips (controls). Confocal laser scanning microscopy images showed that MC3T3‐E1 cells did not spread as extensively on the sandblasted and acid‐etched and bioactive glass‐abraded surfaces as they did on control surfaces. However, for 45S5‐ and Zn4‐treated samples, the cells spread most at the 24 h time point and changed their morphology to more spindle‐like when cultured further. Air‐abrasion with bioactive glass and inert glass was shown to have a significant effect on the wettability and surface free energy of the surfaces under investigation. Osteoblast cell proliferation on sandblasted and acid‐etched titanium discs was enhanced by air‐abrasion with 45S5 bioactive glass and experimental Zn4 bioactive glass compared with air‐abrasion with inert glass or no air‐abrasion.

Background: The increasing demand for bone implants with improved osseointegration properties has prompted researchers to develop various coating types for metal implants. Atomic layer deposition (ALD) is a method for producing nanoscale coatings conformally on complex three-dimensional surfaces. We have prepared hydroxyapatite (HA) coating on titanium (Ti) substrate with the ALD method and analyzed the biocompatibility of this coating in terms of cell adhesion and viability. Methods: HA coatings were prepared on Ti substrates by depositing CaCO3 films by ALD and converting them to HA by wet treatment in dilute phosphate solution. MC3T3-E1 preosteoblasts were cultured on ALD-HA, glass slides and bovine bone slices. ALD-HA and glass slides were either coated or non-coated with fibronectin. After 48 h culture, cells were imaged with scanning electron microscopy (SEM) and analyzed by vinculin antibody staining for focal adhesion localization. An 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) test was performed to study cell viability. Results: Vinculin staining revealed similar focal adhesion-like structures on ALD-HA as on glass slides and bone, albeit on ALD-HA and bone the structures were thinner compared to glass slides. This might be due to thin and broad focal adhesions on complex three-dimensional surfaces of ALD-HA and bone. The MTT test showed comparable cell viability on ALD-HA, glass slides and bone. Conclusion: ALD-HA coating was shown to be biocompatible in regard to cell adhesion and viability. This leads to new opportunities in developing improved implant coatings for better osseointegration and implant survival.

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