Coprinus comatus, well-known as Shaggy Ink Cap mushroom, is potential herbal medicine. Synthetic medicines sometimes cause side effects; therefore, it is necessary to innovate with herbal medications with minimal side effects. The study evaluated in vitro and in vivo treatments to evaluate the antioxidant effect and activity of C. comatus. The in vivo treatment was conducted using six groups of Wistar rats (n = 24). Group 1 healthy control (HC), groups 2–6 received 45 mg/Kg BW of streptozotocin once, group 2 just streptozotocin-induced (NC), group 3 was given 45 mg/kg BW of metformin (PC), groups 4–6 were given 250 (T1), 500 (T2), and 750 mg (T3) of C. comatus extract for 14 days, and the in vitro was conducted using an antioxidant oxidant assay. Data were analyzed using analysis of variance and Duncan's multiple range tests. Based on qualitative analysis, C. comatus mycelium extract contained polyphenol, flavonoids, terpenoids, and fruiting body extract had flavonoids, alkaloids, and saponins. The in vitro analysis showed that the mycelium extract had an antioxidant activity by inhibiting free radicals up to 58.51% with an IC50 value of 72.77 mg/L. The in vivo treatment using C. comatus fruiting body extract showed that it could increase the endogenous antioxidant levels of GPx, SOD, catalase and reduce MDA levels (p 0.05). The most effective dose of C. comatus extract is 500 mg. This research has shown the potential of mycelium and C. comatus fruitbody extract as an antioxidant supplement in a diabetic rat model.
Diabetes mellitus (DM) is a metabolic disease with an increasing prevalence. An increase in free radicals and AGEs can cause glomerular cell damage which can lead to inflammation and DM nephropathy. WHO has recommended DM treatment by using herbal medicines that have minimal side effects and have a lot of biological activity to prevent complications. Coprinus comatus (O.F. Mull.) is known as immunomodulatory, anti-inflammatory and antidiabetic agent. The research used C. comatus cultivated in Cianjur. The basidiome of this mushroom has slightly oval and small with height of 8-12 cm and thickness of 2-3 cm. The research included six groups of male Wistar rats: Group 1 received no treatment, Groups 2–6 were administered 45 mg/kg BW streptozotocin once, Group 3 was administered 45 mg/kg BW metformin, Groups 4, 5, and 6 were administered 250, 500, and 750 mg/kg BW of C. comatus fruit body ethanol extract respectively for 14 days. The superoxide dismutase (SOD), malondialdehyde (MDA), hepcidin and β2 microglobulin (B2M) levels were evaluated. Data were analyzed using analysis of variance and Duncan’s multiple-range tests. The results showed that dose of 500 mg was effective in increasing SOD and decreasing hepcidin, B2M and MDA levels.
Pendahuluan Kolesterol merupakan lemak hasil metabolit yang mengandung senyawa sterol dan banyak terkonsentrasi pada membran sel di dalam tubuh serta sistem sirkulasi.
Tujuan Mengetahui kadar Kolesterol total pada ibu rumah tangga (IRT) berdasarkan kategori rentang usia 25 – 80 tahun dan hubungan peningkatkan kadar Kolesterol total dengan usia IRT.
Metode Penelitian adalah deskriptif analitis dengan pendekatan corss-sectional method, dengan total sampel 30 dan teknik pengambilan sampel adalah purposive sampling method. Data hubungan level kolesterol total dan usia dianalisis secara statistik dengan analisis bivariate dengan indeks pearson correlation pada SPSS software.
Hasil studi menunjukkan IR rata-rata level kolesterol 198.07 mg/dL, dan rata-rata usia 47.87 tahun. Terdapat 50% responden memiliki kadar kolesterol baik, 26.67% kategori perbatasan, dan 23.33% kategori bahaya dengan level kolesterol tertinggi 297 mg/dL dan terendah 130 mg/dL.
Kesimpulan Kadar kolesterol total pada IRT di Kelurahan Tanjung, Purwokerto Selatan menunjukkan bahwa terdapat hubungan yang kuat antara peningkatan kadar kolestrol di dalam tubuh dengan penambahan usia
Kebersihan air untuk kepentingan masyarakat di Desa Mandiraja Wetan, khususnya RT 07 RW 02 menjadi hal yang sangat penting, mengingat penggunaan air yang banyak digunakan untuk konsumsi, usaha, dan MCK. Pentingnya kebersihan air tandon air perlu di evaluasi dan investigasi serta dimonitoring agar kualitas air di tandon air dapat terjaga. Metode pengabdian masyarakat ini adalah dengan wawancara langsung dan pemberian kuisioner berupa pertanyaan. Hasil wawancara kemudian disajikan dalam bentuk data persentase dan histogram (deskriptif analitis) yang dibuat dengan microsoft excel. Tujuan dari pengabdian masyarakat ini adalah untuk mengetahui profil dan status kebersihan tandon air serta agar warga dapat lebih memperhatikan kebersihan tandon air tersebut guna meningkatkan status Kesehatan. Hasil pengabdian masyarakat ini menunjukan bahwa ada sekitar 60 kepala keluarga (KK), dimana 75% masyarakatnya memiliki tandon air berupa toren, dan hanya 16,7% tidak memiliki tandon air, dan sisanya 8,3% tandon air dalam bentuk cor. Selain itu 40% masyarakat selalu membersihkan tandon air pada rentang 6-12 bulan, dan hanya 20% yang membersihkan pada rentang 1-3 bulan dan warga membersihkan tandon air dengan frekuensi 1-2 kali yaitu 52% dan hanya 5% 4 dengan 4 kali.
Escherichia coli and Staphylococcus aureus are bacteria that often cause health problems and diseases in humans, such as digestive (diarrhea) and skin (boils) disorders. Gentamicin is one of the most widely used antibiotics, which belongs to the aminoglycoside group. It is often used for treating minor bacterial and moderate fungal infections. Therefore, this study aims to determine the inhibition and effectiveness of gentamicin in inhibiting the growth of E. coli and S. aureus as well as to assess the pattern of sensitivity of these bacteria to the antibiotic. A completely randomized design method was used with one-way ANOVA, and further testing was carried out using Duncan's test. A total of 10 experimental units were used for each bacterium with two replications, after which the data were analyzed using SPSS v.25.0 software. The results showed that gentamicin could inhibit the growth of E. coli with the lowest and highest ZH values of 17 mm and 22.5 mm, respectively. It also had an inhibitory effect on S. aureus with the smallest ZH value of 15.5 mm and highest of 18.5 mm. The percentage of gentamicin inhibition against E. coli and S. aureus was 90% and 60% sensitive. Furthermore, the percentage of intermediate inhibition category for E. coli was 10% and 40% for S. aureus.
Soybean (Glycine max L. Merr.) is the third food crop commodity after rice and maize in Indonesia. This plant is also known as the most important source of vegetable protein, which is relatively inexpensive, but a decrease in soybean productivity can occur due to infection with disease-causing pathogens, one of is Cercospora kikuchii which causes Cercospora leaf blight (CLB). The research objectives were to determine the anatomical resistance and disease severity of soybean cultivars against CLB. The method was an experiment with a completely randomized design (CRD) factorial pattern; factor 1 being soybean cultivars (Dering, Slamet, Grobogan, Wilis) and factor 2, namely pathogen inoculation (0 conidiospores/mL and 105 conidiospores/mL). Anatomical method preparations using paraffin, staining with 1% safranin. Disease criteria are based on the council of scientific and industrial research (CSIR) assessment method. Data were analysis used analysis of variance (p0.05) and the least significance difference (LSD). The results showed that Dering and Slamet cultivars had the largest cuticle, epidermis, and palisade ratios and the smallest stomata length and width with the largest number of stomata and trichomes compared to Grobogan and Wilis. The disease severity (DS) of the cultivars Dering 14.6%, Slamet 24.64%, Grobogan 24.80% (classified as a resistant with low infection), while Wilis cultivar was 31.08% as a moderately susceptible cultivar with moderate infection. The novelty of soybean cultivar selection against CLB is important and its effectiveness for increasing soybean productivity. Dering, Slamet and Grobogan are likely to be further developed with their resistance to CLB disease.
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