Penelitian ini bertujuan untuk menganalisis kandungan vitamin C rotifer, artemia dan larva rajungan setelah diperkaya dengan vitamin C dan menganalisis pemberian vitamin C terhadap sintasan, rasio RNA/DNA, kecepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea. Serta menentukan dosis optimum vitamin C yang menghasilkan sintasan, rasio RNA/DNA, ke-cepatan metamorfosis dan ketahanan stres larva rajungan stadia zoea yang terbaik. Penelitian ini dilaksanakan pada bulan Mei sampai Juni 2020 di unit pembenihan kepiting dan rajungan Balai Perikanan Budidaya Air Payau (BPBAP) Takalar. Analisis Rasio RNA/DNA dilakukan di Laboratorium Uji Fisika Kimia BPBAP Takalar. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa pemberian vitamin C pada rotifer dan artemia dapat meningkatkan kandungan rotifer, artemia dan larva rajungan. Pemberian vitamin C pada rotifer dan artemia terhadap larva rajungan stadia zoea dapat meningkatkan sintasan, kecepatan metamorfosis dan ketahanan stres larva rajungan dan Dosis vitamin C 250 ppm yang terbaik pada pemeliharaan larva rajungan stadia zoea. This study aimed to analyze the content of vitamin C rotifer, artemia and small crab larvae after enriched with vitamin C and to analyze the provision of vitamin C to survival, RNA / DNA ratio, metamorphosis rate and stress resistance of crab larvae in zoea stage. Also, it aimed to determine the optimum dose of vitamin C which produces the best survival rate, RNA/DNA ratio, metamorphosis speed and stress resistance of crab larvae in zoea stage. This research was conducted from May to June 2020 in the hatchery unit of the Brackish Water Cultivation Fishery Center (BPBAP) Takalar. RNA / DNA ratio analysis was carried out at the Laboratory of Chemical Physics Testing of BPBAP Takalar. The research design used a completely randomized design (CRD). The results showed that giving vitamin C to rotifers and brine shrimp increased the content of rotifers, brine shrimp and crab larvae. Giving vitamin C to rotifers and artemia to larvae of zoea stadia crabs can increase survival, metamorphosis speed and stress resistance of crab larvae and the best dose of vitamin C 250 ppm in the maintenance of zoea stadia crab larvae.
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