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The current study was aimed at evaluating the antihyperalgesic effects of lignans (phyllanthin and hypophyllanthin) and tannin (corilagin) rich three standardized extracts of Phyllanthus amarus in a model of chronic musculoskeletal inflammatory pain. Three percent carrageenan injected in the gastrocnemius muscle produced hyperalgesia to mechanical and heat stimuli ipsilaterally, which spreads to the contralateral side within 7 to 9 days. To investigate the effects on chronic thermal and mechanical hypersensitivity, three extracts of P. amarus in three doses (100, 200, and 400 mg/kg) were administered to animals intraperitoneally from 14th day to 22nd day after intramuscular injection of carrageenan. It was observed that intraperitoneal administrations of Phyllanthus extracts showed antihyperalgesic activity, as they elevated thermal and mechanical threshold, which was supported by histopathological observations along with reduction in prostaglandin E2 (PGE2) concentration. In conclusion, we strongly suggest that the observed antihyperalgesic and antiinflammatory effects of P. amarus in current pain model are mediated via spinal or supraspinal neuronal mechanisms, mainly by inhibition of PGE2. Modulation of chronic muscular inflammation may be due to presence of phytoconstituents like phyllanthin, hypophyllanthin, and corilagin, which offers a promising means for treatment of chronic muscle pain.

In the present carrageenan induced chronic pain model we have determined the role of analgesics in the reversal and inhibition of the state of chronic hyperalgesia. While considering the characterization of the present model our observations suggest the importance of a spinal COX-2 mechanism, a spinal action of systemically delivered drugs in the face of peripheral inflammation.

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Objective: To evaluate pharmacognostic, physicochemical and preliminary phytochemical of investigation of root bark of Caesalpinia bonducella. Methods: The present work embodies the investigations of pharmacognostic standards including macroscopic study, powder analysis and histochemical test along with physico chemical parameter study, fluorescence analysis, qualitative and quantitative chemical assay carried out as per methods recommended by the World Health Organization and standard laboratory procedures. The study will provide referential information for the correct identification of the crude-drug. Result: The detailed microscopy revealed the presence of cork, cortex, stone cell medullary ray’s calcium oxalate crystal, starch grains, presence of tannins and alkaloids. Preliminary phytochemical screening of these plant materials revealed that presence of alkaloids, saponins, flavonoids, steroids, phytosterols and carbohydrates in aqueous and ethanol extracts. Conclusion: Thus, it was thought worthwhile to explore this plant on the basis of its standardization parameters. The study will provide referential information for the correct Identification of the crude-drug.