The objective of this study was to determine the correlation between spermatozoa morphometry and testosterone concentration in the epididymal caput, corpus, and cauda of aceh cattle. The epididymal sperm swab slides were stained using Williams's staining to examined the morphology and morphometry of spermatozoa whereas the level of testosterone from epididymal caput, corpus, and cauda extract was measured using enzyme-linked immunosorbent assay (ELISA). The data of sperm morphology were presented descriptively whereas the data of sperm morphometry and testosterone concentration were analyzed using one way ANOVA. Pearson correlation test was conducted to know the correlation between the total length of spermatozoa (TLS) and testosterone concentration in three parts of epididymis. The result showed that the sperm morphology in caput, corpus, and cauda of epididymis was not different statistically. The TLS of aceh cattle at caput, corpus, and cauda epididymis were 69,70±0,8 µm, 70,90±0.39 µm, and 72,98±0.74 µm respectively and statistically different (p<0.05). In addition, the concentration of testosterone in caput, corpus, and cauda epididymis were 9,92±3,76 ng/g, 16.96±13,22 ng/g, and 15,63±11.80 ng/g respectively and also not different among three parts of epididymis (p>0.05). The positive correlation (r= 0.413, p= 0.416) was found between TLS and testosterone concentration at caput whereas in corpus and cauda those correlation were negative and not different (corpus: r= -0.638, p= 0.172 and cauda: r= -0.719, p= 0,108). In conclusion, the longest-sized spermatozoa found in the cauda epididymis are mature spermatozoa where the maturation process optimally takes place in caput epididymis. Testosterone found in the epididymis of aceh cow can be used as an alternative source of testosterone.
This research aimed to study the histology of the digestive system of the watersnake (Enhydris enhydris). The digestive system taken was the esophagus, stomach, frontal small intestine and the back of the large intestine from three watersnakes. The samples were then made into histological preparations with hematoxylin-eosin (HE) staining and observed exploratively. The results showed that the digestive system of the watersnake was composed of layers of tissue, namely the mucosa, tunica submucosa, tunica muscularis, and serous tunica. Mucosal mucosa consisted of the lamina epithelium, lamina propria, and mucous lamina muscularis. The submucosal tunica consisted of connective tissue with blood vessels, lymph, and nerves. The muscular tunica was composed of circular muscles and elongated muscles. The serous tunica consisted of a thin layer of connective tissue that was covered by a thin layer of the mesothelium (mesothelium). The histological structure of the snake digestive system is not much different from the reptile digestive system. However, in the small intestine of the snake, Brunner, and liberkhun glands are not found. Lamina epithelial in the small intestine of the snake is composed of the layered cylindrical epithelium.
The herb extract of Euphorbia hirta L. have been reported as sources of inexpensive antioxidant that can be used as potential cosmetic product. Therefore, the aims of this study were to formulate E. hirta L. extract loaded cream and to assess the antioxidant activity of loaded cream. First, E. hirta L. was prepared with maceration process using water as solvent (1:5). Afterwards, the obtained extract was subjected to a lyophilisation process. The extract was tasted for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) for suitable concentration of the extract incorporated into the cream, which was 10%w/w. The cream was formulated as o/w emulsion and stearic acid was selected as an emulsifier. The extract loaded cream was tested pH, viscosity, and antioxidant. The freshly prepared extract loaded cream was dark green and no phase separation was observed after centrifugation at 3,000 rpm for 30 min. They had acidic pH (4.4-5.1), considered acceptable for skin application, especially in skin face. The DPPH measurement revealed that the formulation had potential antioxidant activity with %SCV more than 80% at 100 µg/mL. In conclusion, topical o/w cream containing extract was successfully prepared with substantial antioxidant activity and was displayed a potential use in cosmetic formulations, especially antiaging products.
This study aims to determine the percentage of body weight deviation of Aceh bull using the Lambourne formula and compared to the actual weight of Aceh bull. The sample used in this study was 50 Aceh bull aged 2-2.5 years in BPTU-HPT Indrapuri Aceh. The parameters observed were Body Length (BL), Chest Circumference (CC), and Body Weight (BW). BL measurement procedure was conducted by measuring the distance between the lateral humeral tuberculum to the ischiadium tuberculum using a measuring stick. CC was measured by encircling the chest cavity which is behind the shoulder bone joint (os scapula) using measuring tape. BW measurement procedure was carried out in two ways, first with digital livestock scales and second with Lambourne formula. The percentage of body weight deviation of Aceh bull using Lambourne formula to the actual’s Aceh bull body weight was 3.41% with regression equation was Y = -350.30 + 2.27x1 + 2.06x2.
This study aimed to analyze the muscle microstructure of longissimus dorsi Brahman cross (BX) cattle slaughtered at the slaughterhouse of North Aceh District. The meat samples for the study were taken from three male BX cattle aged 18-30 months with body weights ranging from 400 kg to 500 kg and the average body condition scores (BCS) of 3-4. The muscle used was the longissimus dorsi muscle. The mean (±SE) diameter and cross-sectional area (CSA) of muscle fibers and the percentage of fast fibers in the longissimus dorsi BX muscle were 71.69±1.23 µm, 4263.43±305.69 µm2 , and 48.17±8.81%, respectively. This study concluded that the longissimus dorsi muscle of the BX has relatively large muscle fibers, some of which in each fascicle are composed of fast muscle (fast fiber/type II).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.