RESUMOOs carrapatos causam danos aos seus hospedeiros tanto pela ação espoliadora e ingestão de sangue quanto pela transmissão de agentes patogênicos, sendo também responsáveis por elevados gastos com produtos carrapaticidas, mão-de-obra e equipamentos para o controle (CORDOVÉS, 1997 The tick-borne disease (TBD) brings great damages to cattle breeding. The most important etiologic agents are Babesia bigemina, B. bovis and Anaplasma marginale, being the tick Boophilus microplus the main vector. This work reports the occurrence of natural infection by hemoparasites of TBD in 36 calves with high ticks natural infestation submitted to chemoprophylaxis with 30 days year-old. The blood smears from animals of different ages were analized and were found B. bigemina (33.3%), B. bovis (11.1%) and A. marginale (13.9%). Six animals had clinical symptoms (16.7%) and one dead (2.8%). The number of clinical cases ocurred in consequence of an association of factors as high infestation of ticks and low passive immunity in period that calves had not developed enough active immunity.

(Azulay et al. 1991, Yoshinari et al.1993a,b, Yoshinari et al. 1997), sendo denominada de borreliose de Lyme-like por Yoshinari et al. (2000). Em animais, estudos sorológicos foram realizados em bovinos (Ishikawa 1996(Ishikawa , 2000, cães (Soares et al.

Some tick species have been described parasitizing buffaloes, but reports from Brazil are rare. This study aimed to ascertain the species of ticks that parasitize buffaloes in the state of Rio de Janeiro. Seventeen farm properties were visited between June 2008 and December 2009, and buffaloes were inspected in relation to infestation. The ticks were identified and a semi-structured questionnaire on parasitism and treatment against ticks was filled out through interviews with the owners. The species identified were Rhipicephalus (Boophilus) microplus, Dermacentornitens and Amblyommacajennense. It was noted that 82.3% (14/17) of the properties visited presented animals infested with ticks, although 76.5% (13/17) had been administering acaricide treatment. Buffaloes in the state of Rio de Janeiro are often parasitized by ticks, especially in the larval and nymph stages.

ResumoBorrelia burgdorferi é uma espiroqueta transmitida por carrapatos, de ampla distribuição geográfica, que acomete o homem e animais domésticos, tendo os animais silvestres como reservatórios. O presente estudo teve como objetivo relatar a ocorrência de eqüinos soropositivos para B. burgdorferi, por meio de inquérito soroepidemiológico utilizando o ensaio de imunoadsorção enzimática (ELISA) indireto. Procedeu-se a análise sorológica de 747 soros de eqüinos, onde 431 pertenciam ao município de Três Rios e 316 ao de Vassouras, ambos do estado do Rio de Janeiro. Um total de 212 (28,4%) animais foram positivos ao ensaio. O resultado observado foi similar aos descritos para animais no Brasil e Estados Unidos da América, tendo sido verificado que os animais apresentaram anticorpos homólogos anti-Borrelia burgdorferi stricto sensu, corroborando com a ocorrência de Borrelia sp. em eqüinos no estado do Rio de Janeiro.Palavras-chave: soroepidemiologia, borreliose de Lyme, ELISA indireto. AbstractLyme Borreliosis is a widely distributed spirochetosis hosted by wild animals, which transmitted to domestic animals as well as man by ticks. The present study aims to observe the occurrence level of positive tested equines to Borrelia burgdorferi, on two municipalities of Rio de Janeiro State, Brazil, by seroepidemiologic inquiry using the indirect Enzime-Linked Immunosorbent Assay. A serologic analysis was performed on 747 equines serum specimens, which 431 were from Três Rios and 316 were from Vassouras municipality. For this test, 212 (28.4%) animals were positive. The results observed were similar to those described for animals in Brazil and United States of America, confirming that the animals of the studied municipalities showed anti Borrelia burgdorferi homologs antibodies.

Detection of anti-Borrelia burgdorferi antibodies in buffaloes (Bubalus bubalis) in the state of Pará, Brazil AbstractThis study aimed to investigate the frequency of homologous antibodies of IgG class against Borrelia burgdorferi in buffaloes in the state of Pará, Brazil. Blood serum samples from 491 buffaloes were analyzed by means of the indirect ELISA test, using crude antigen produced from a cultivar of the North American strain G39/40 of B. burgdorferi. There were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% (232/284) originating from Marajó Island and the 86.96% (180/207) from the continental area of the state of Pará. In all the municipalities studied, the frequency of positive findings of antibodies against B. burgdorferi among the animals ranged from 63.6% to 92.9%. The high numbers of seropositive animals can be explained by the frequent presence of the tick Rhipicephalus (Boophilus) microplus, and by the possible existence of spirochetes of the genus Borrelia infecting buffaloes in the region studied, although specific studies are needed to confirm this relationship. These factors suggest that a cross-reaction exists between the North American strain G39/40 of B. burgdorferi, which is used as an antigenic substrate, and the species of Borrelia spp. that possibly infects buffaloes in the state of Pará.Keywords: Buffaloes, Borrelia spp., epidemiology, serology. ResumoEste estudo teve como objetivo investigar a frequência de anticorpos homólogos da classe IgG contra Borrelia burgdorferi em búfalos do estado do Pará. Amostras de soro de 491 búfalos foram analisadas por meio do teste ELISA indireto, utilizando antígeno bruto produzido a partir do cultivo da cepa norte americana G39/40 de B. burgdorferi. Foram encontrados 412 soros positivos (83,91%), não havendo diferença estatística significativa entre os 81,69% (232/284) animais positivos provenientes da Ilha de Marajó e os 86,96% (180/207) da base continental do estado do Pará. Em todos os municípios estudados os animais apresentaram frequência de anticorpos contra B. burgdorferi, com positividade variando de 63,6% a 92,9%. O alto número de soropositivos pode ser explicado pela frequente presença do carrapato Rhipicephalus (Boophilus) microplus e pela possível existência de espiroquetas do gênero Borrelia infectando búfalo na região estudada, embora novos estudos sejam necessários para a confirmação desta relação. Estes fatos sugerem reação cruzada entre a cepa americana G39/40 de B. burgdorferi utilizada como substrato antigênico e a espécie de Borrelia spp. que possivelmente infecta bubalinos no estado do Pará.