It was evaluated the effect of fungicides and the microbial control agent Trichoderma harzianum on the inhibition of the carpogenic and ascospore germination of 2010-11, reduziram em 75,7 e 77,6%, respectivamente.
RESUMOPalavras-chave adicionais: ferrugem alaranjada, liofilização, sílica gel, Saccharum officinarum, reidratação.With the aim of evaluating preservation methods of Puccinia kuehnii urediniospores, two bioassays were conducted: the first one (B1) with different methods of dehydration and the second one (B2) with different methods rehydration. B1 and B2 differed in the inclusion of silica gel granule in B1 for preservation in microcentrifuge tubes. Leaves with symptoms of orange rust were harvested from the sugarcane cultivar SP89 1115. P. kuehnii urediniospores were extracted from the leaves with the aid of a vacuum bomb. Later, they were placed in microcentrifuge tubes. Treatments for B1 were: l: dehydration in silica gel, lyophilization and without dehydration, ll: room temperature (20°C), refrigerator (5°C), freezer (-20°C) and deep-freezer (-80°C). Treatments for B2 were: l: dehydration in silica gel and without dehydration, ll: room temperature (20°C), refrigerator (5°C), freezer (-20°C) and deep-freezer (-80°C), lll: with and without rehydration in the evaluations. The initial germination was carried out for both experiments, other assessments were made at 15 and 30 days of storage and then at every 30 days until 180 Tibolla, F.; Sumida, C.H.; Peitl, D.C.; Canteri, M.G., Castro, A.M.C. In vitro preservation methods of Puccinia kuehnii urediniospores. Summa Phytopathologica, v.38, n.3, p.198-203, 2012. days. Urediniospore suspensions were prepared in water and a 0.1-mL aliquot was transferred to Petri dishes containing agar-water (15 g L -1 ). They remained at 20°C in the dark. To assess viability, the count of 200 urediniospores/plate was performed. The data were subjected to nonparametric Kruskal-Wallis analysis of variance and complemented by Dunn's test. Results showed that viability decreased with time, and the best treatments reached 27.6% and 6.6% at 30 days, and 12.0% and 1.9% at 60 days for B1 and B2, respectively. Dehydration method in silica gel followed by storage at -80°C was the only treatment that presented viable urediniospores (1.2%) at 180 days for B1. For B2, the best method was preservation with dehydration, followed by storage at 5°C, without rehydration. This method showed the best germination percentage at 120 days (0.4%). Influence of the factor thermal shock was not observed in the recovery of viable urediniospores in B2. Inclusion of silica gel granules in the microcentrifuge tube allowed the recovery of viability of urediniospores at 180 days.Com o objetivo de avaliar métodos de preservação de urediniósporos de Puccinia kuehnii, conduziram-se dois bioensaios sendo o primeiro (B1) com diferentes métodos de desidratação e o segundo (B2), com diferentes métodos de reidratação. Em B1 foi adicionado um grânulo de sílica gel para preservação dos urediniósporos nos tubos de microcentrífuga. Foram coletadas folhas com sintomas de ferrugem alaranjada, P. kuehnii, da cultivar de cana-de-açúcar SP89 1115. Os urediniósporos do agente causal de ferugem foram extraídos das folhas com o auxí...
ResumoAs ferrugens contribuem para perdas de rendimentos na cultura da cana-de-açúcar. O presente estudo teve como objetivo avaliar o período de viabilidade dos urediniósporos de Puccinia kuehnii e a influência de extratos aquosos em sua germinação in vitro. Para o primeiro bioensaio (B1) foram coletadas folhas com sintomas de ferrugem alaranjada, das cultivares de cana-de-açúcar SP89 1115 e RB72 454. Estas foram armazenadas em câmara úmida, até as datas das avaliações de germinação. Os urediniósporos foram coletados das folhas e preparou-se uma suspensão em água destilada. Uma alíquota de 0,1 mL foi transferida para placas de Petri contendo meio ágar-água (15g L -1 ), mantidas a 20ºC, no escuro por 24 horas. As avaliações foram feitas aos 0, 1, 2, 4, 8 e 16 dias após a coleta das folhas, com cinco repetições por avaliação. No Segundo bioensaio (B2) os urediniósporos foram coletados de folhas com sintomas de ferrugem alaranjada da cultivar SP89-1115. Foi preparada uma suspensão em água destilada e uma alíquota de 0,1 mL foi transferida para placas de Petri contendo meio ágar-água e 1 mL de extratos aquosos de folhas de cana-de-açúcar. Os tratamentos constituíram-se em dois extratos aquosos: variedade suscetível RB72 454 e resistente RB86 7515 nas diluições de 1:1; 10 -1 ; 10 -2 ; 10 -3 . Foram realizadas 4 repetições. Para ambos os bioensaios avaliou-se a germinação de 200 urediniósporos/placa. Os resultados de B1 demonstraram que para a variedade SP89 1115 a porcentagem de germinação foi significativamente inferior à variedade RB72 454, respectivamente 26,4% e 93,1% na data da coleta a campo e 24,8% e 32,4% aos 16 dias após a coleta. Em B2 a porcentagem de germinação dos urediniósporos que receberam a suspensão aquosa da variedade resistente, foi em média de 7,6 pontos percentuais menor do que os que receberam a suspensão aquosa da variedade suscetível. Palavras-chave: Ferrugem alaranjada, urediniósporos, compostos foliares, Saccharum officinarum AbstractRusts contribute to yield losses in crops of sugarcane. The present study was to evaluate the viability urediniospores of Puccinia kuehnii over time and the influence of aqueous extracts in vitro germination. Leaves with symptoms orange rust, cultivar SP89 1115 and RB72 454, were collected for the first bioassay (B1). These were stored in a humid chamber, to date evaluations. Urediniospores were collected from the leaves, and suspended in distilled water 0.1 ml was prepared they were transferred to Petri dishes containing agar-water, maintained at 20°C in the dark for 24 hours. Evaluations were made
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