ABSTRACT. Life cycle of Eimeria krijgsmanni-like coccidium isolated from the feces of naturally infected mice purchased from commercial sources was examined. The parasite was purified by single oocyst isolation and maintained by passage in the mice before experiments. The sporulated oocysts were ovoid or ellipsoid, measuring 19.3 × 14.8 μm on average. One or two small polar granules were present. Micropyle and oocyst residuum were absent. Sporocysts were ellipsoid, measuring 11.6 × 7.2 μm on average with a small Stieda body and sporocyst residuum. Six groups of respective 5 mice (4-week-old) were inoculated with doses varying from 2.0 × 10 1 to 10 6 oocysts. All the mice examined began to shed oocysts from 7 day postinoculation (PI) and their maximum number of oocysts per gram of feces were 10 6 on day 8 PI. Patency was 6 or 7 days. This parasite had severe virulence to the mice that is, the mice given 10 6 oocysts showed anorexia, diarrhoea and rough hair from 1 day and all of them died on day 3 PI. The mice given 10 3 or more oocysts showed the clinical signs described above from day 5 and 4 of them received 10 5 died on day 9 or 10 PI. The parasites occurred within the epithelial cells of cecum, colon and rectum of infected mice. Sporozoites, 13.9 × 3.0 μm, with two large refractil bodies on side of the nucleus located subcentrally were observed on day 1 and 2 PI. Merozoites were first observed at 24 hr PI, and sexual stages were found from 4 day PI. No parasites were detected in the small intestine and mecenteric lymph nodes. Fourteen species of murine Eimeria have been described, except E. pragensis which of E. falciformis [7]. However, the life cycles of 9 species other than E. contorta, E. falciformis, E. falciformis var. pragensis, E. ferrisi, E. papillata and E. vermiformis [1,5,7,8, 10,11] were not examined well. We isolated a virulent strain of Eimeria from the naturally infected mice purchased from commercial sources. The oocysts of this coccidium were morphologically similar to those of E. krijgsmanni [9,12]. However, the biological characteristics of E. krijgsmanni except oocyst morphology have not yet been clarified [9,12]. Therefore, the exogenous and endogenous stages of the coccidium were examined and compared with other eimerian species described in mice. MATERIALS AND METHODS Parasite:The parasite used in this study was first isolated from the faeces of naturally infected mice purchased from commercial sources. It was purified by a single oocyst isolation method [3] and maintained in the laboratory by routine passage through coccidia-free mice. Passaged oocysts were sporulated in 2% potassium dichromate solution at 25°C and stored at 4°C until use.Experimental animals: Mice used were of the SPF ICR strain aged 4-week-old. They were obtained from a commercial source, raised in a coccidia-free environment, and examined for oocysts in their faeces by the sugar floatation method (specific gravity of sugar solution, 1.266) prior to experimentation.Experimental procedures: Oocysts were washed in di...
The transformation of sporocysts in oocysts of Eimeria caviae, E. intestinalis, E. nieschulzi, E. separata, E. tenella, Isospora canis, I. heydorni, and Toxoplasma gondii by heat treatment was examined. Fresh unsporulated oocysts from these species were heated at 50 degrees or 55 degrees C for less than 5 min and cultivated at 25 degrees C for 1 week. No transformation of sporocysts was observed in the sporulated oocysts following heating at 55 degrees C, but sporulated oocysts of Tyzzeria and Caryospora types were found in some I. canis and I. heydorni oocysts after heating at 50 degrees C followed by cultivation. A few sporulated oocysts of the Isospora type were observed in E. intestinalis oocysts after cultivation. All transformed sporulated oocysts contained eight sporozoites. In contrast, the number of sporocysts varied from two to 0. These results indicate that sporocyst formation in oocysts was affected by heat treatment. The relationship between the total numbers of sporozoites and sporocysts in the transformed oocysts was consistent with that of the "Octozoic" group classified by Hoare.
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