Life Cycle of Eimeria krijgsmanni-Like Coccidium in the Mouse (Mus musculus)

Matsui, Toshihiro; Fujino, Takashi; Kobayashi, F; Morita, Tatsushi; Imai, Soichi

doi:10.1292/jvms.68.331

Cited by 9 publications

(10 citation statements)

References 8 publications

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“…by the sugar flotation method (Matsui et al 2006), and were kept in coccidian-free conditions until use. The animal experiments in the present study were carried out in accordance with the approval from the animal ethics committee at Kagoshima University (Nos.…”

Section: Methodsmentioning

confidence: 99%

“…krijgsmanni was obtained from the Division of Tropical Diseases and Parasitology, Department of Infectious Diseases, Kyorin University School of Medicine, and maintained by routine passage through ICR mice in the Laboratory of Parasitology, Joint Faculty of Veterinary Medicine, Kagoshima University (Matsui et al 2006). Oocysts were collected from the feces of infected mice, allowed to sporulate in 2% potassium dichromate solution at 25°C for 5 days, and stored at 4°C until use.…”

Section: Methodsmentioning

confidence: 99%

“…Although 14 murine Eimeria species have been described to date, only a few species such as E. vermiformis or E. papillata are well-documented. Recently, we have genetically characterized E. krijgsmanni and reported details of the infection depending on the immune status of mice (Hashimoto et al 2014 Matsui et al 2006;Takeo et al 2014). We attempted to evaluate E. krijgsmanni as a murine model for testing the efficacy of anti-parasitic agents including a coccidiostat commonly used in livestock.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences

Ghorashi

Tavassoli

Peters

et al. 2016

Acta Parasitologica

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Murine Eimeria spp. have been used as effective models of disease instead of large mammalian hosts such as cattle. We attempted to establish in vivo and in vitro assays using a murine intestinal protozoan, Eimeria krijgsmanni, which we previously isolated, to test anti-parasitic agents. Consequently, when mice were treated with sulfur drugs or toltrazuril, which are commercially available for livestock. Furthermore, sporulated oocysts and excysted sporozoites of E. krijgsmanni were treated with naturally occurring substances (lactoferrin, longicin, and curcumin). Although exposure to these substances did not affect oocyst infectivity, sporozoite viability decreased by 60% with longicin. However, direct injection of sporozoites treated with longicin into mice ceca did not result in any changes in the oocyst shedding pattern compared with control mice. The results suggest that E. krijgsmanni could be resistant to these anti-parasitic agents and might therefore have different characteristics to other apicomplexan parasites.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences

Ghorashi

Tavassoli

Peters

et al. 2016

Acta Parasitologica

View full text Add to dashboard Cite

show abstract

“…The presence of oocysts was examined by the sugar flotation method. If positive, the number of oocysts per gram of feces (OPG) value (>10 3 ) was estimated (Matsui et al 2006).…”

Section: Methodsmentioning

confidence: 99%

“…However, these studies are limited to wellknown Eimeria species such as Eimeria papillata or E. falciformis. Recently, Matsui et al (2006) identified a species isolated from the feces of naturally infected mice as E. krijgsmanni morphologically and biologically. Additionally, our another research showed differences of the drug sensitivity from other mouse Eimeria spp.…”

Section: Introductionmentioning

confidence: 99%

Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni

Hashimoto

Tanaka

Matsubayashi

et al. 2014

Acta Parasitologica

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In the present study, infection experiments of E. krijgsmanni using various hosts were conducted to elucidate the host specificity among some animals and the infectivity to mouse strains. According to the results, the infection was not found in most animals, except for rats, in which some oocyst shedding was detected, and there was no significant difference in infectivity among mouse strains. Additionally, oocyst shedding was hardly detectable in a secondary infection to immunocompetent mice, although it was found in immunodeficient mice. These results indicated that only immunocompetent mice could develop adaptive immunity against reinfection by stimuli of the primary infection. Furthermore, the infection experiments were performed with splenic macrophage (Mφ)-depleted mice with a reagent and Beige (Bg) mice known to be a strain of mice with low NK cell activity. No significant effect was found in primary or secondary infections in the Mφ-depleted mice, whereas the mortality rate was clearly increased in Bg mice inoculated with a large number of oocysts. Their oocyst shedding was similar to that of immunocompetent hosts. Taken together, these results suggested that Mφ has only a minor role in the immune response, but the NK cell has an important function in resistance to primary infection of E. krijgsmanni.

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Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice

et al. 2015

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Recently, we have demonstrated the utility of Eimeria krijgsmanni as a novel mouse eimerian parasite for elucidating the biological diversity. The parasite showed notable infectivity to mice with various levels of immune status and susceptibility to antimicrobial agents including coccidiostat. However, the detailed lifecycle of E. krijgsmanni had not yet been determined and this information was lacking in discussion of previous findings. In the present study, we clarified the morphological characteristics of E. krijgsmanni and its lifecycle in normal mice, and examined the effects in immunodeficient mice and lifecycle stage for challenge infections after the primary inoculation. In immunocompetent mice, the lifecycle consisted of four asexual stages and the sexual sages followed by formation of oocysts during the prepatent periods. Interestingly, the second-generation meronts were detected in all observation periods after the disappearance of the other stages. For the challenge infection of immunodeficient mice, all developmental stages except for the second generation meronts were temporarily vanished. This finding suggests a "rest" or marked delay in development and a "restart" of the promotion toward the next generations. The second generation meronts may play an important role in the lifecycle of E. krijgsmanni.

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Life Cycle of Eimeria krijgsmanni-Like Coccidium in the Mouse (Mus musculus)

Cited by 9 publications

References 8 publications

Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences

Phylogenetic relationships among Linguatula serrata isolates from Iran based on 18S rRNA and mitochondrial cox1 gene sequences

Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni

Course of induced infection by Eimeria krijgsmannni in immunocompetent and immunodeficient mice

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