8. Simultaneous Analysis of D-and L-Amino Acids 3259 4. Microdialysis Methods for Glutamic and Ascorbic Acids 3261 5. Conclusions 3263 6. References 3263 Maite Sanz Alaejos was born in Madrid, Spain. She received a M.Sc. in Biological Sciences from the University of La Laguna in 1977 and a M.Sc. in Pharmacy from the same university in 1981. She received her Ph.D. in Pharmacy under the supervision of Prof. Dr. F. J. Garcı ´a Montelongo and of Prof. Dr. J. C. Rodrı ´guez Placeres from the University of La Laguna in 1987. Her thesis work was on the polarographic determination of the stability constants of the simple and mixed coordinated species of Cu(II) with amino acids. Since 1990, she has been Assistant Professor in the Department of Analytical Chemistry, Nutrition and Food Sciences in the University of La Laguna. Prof. Francisco J. Garcı ´a Montelongo was born in Santa Cruz de Tenerife (Canary Islands, Spain). He received his M.Sc. (1961) and Ph.D. (1969) in Chemistry from the University of La Laguna. He was professor of Analytical Chemistry at the University of Bilbao (Spain) and since 1976 in the University of La Laguna. His main research interest is in the fields of biosensors, environmental pollution, and development of new analytical methodologies.
SummaryA group of flavonoids of special interest to wine qualif,/were separated by micellar electrokinetic chromatography (MEKC) with diode array detection. Their separation was optimized as a function of the buffer concentration and pH, the concentration of sodium dodecyl sulfate (SDS) and the applied voltage. Selectivif,/and resolution of the solutes were increased by adding organic solvents to the separation buffer, the best results being obtained at lower concentrations. An optimized buffer with 5% methanol provided optimum separation with regard to efficiency resolution and migration time. The optimized method was applied to the determination of these compounds in wine samples.
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