Optimization of the separation of flavonoids by micellar electrokinetic capillary chromatography: Effect of organic solvents

Abstract

SummaryA group of flavonoids of special interest to wine qualif,/were separated by micellar electrokinetic chromatography (MEKC) with diode array detection. Their separation was optimized as a function of the buffer concentration and pH, the concentration of sodium dodecyl sulfate (SDS) and the applied voltage. Selectivif,/and resolution of the solutes were increased by adding organic solvents to the separation buffer, the best results being obtained at lower concentrations. An optimized buffer with 5% methanol provided optimum separation with regard to efficiency resolution and migration time. The optimized method was applied to the determination of these compounds in wine samples.

A MEKC method for naringenin from natural and biological samples

“…10 Several methods for the determination of naringenin have been described. 17 During the past decade, capillary electrophoresis (CE) has become one of the versatile approaches for highly efficient separation. 14 However, these methods tend to be rather timeconsuming, use toxic solvents, and sometimes require development of extremely complex gradients for the separation.…”

CE methods applied to the analysis of micronutrients in foods

“…The application of MEKC has also been used for the analysis of flavonoids [27,31]. Rodríguez-Delgado et al [31] optimized the separation of quercetin, rutin, myricetin, kaempferol and quercetrin in wine as a function of several factors including pH and buffer composition; SDS and organic solvent concentration.…”

“…Rodríguez-Delgado et al [31] optimized the separation of quercetin, rutin, myricetin, kaempferol and quercetrin in wine as a function of several factors including pH and buffer composition; SDS and organic solvent concentration. A 50 mM concentration of SDS was chosen as a compromise between resolution and separation efficiency in addition to the conditions selected: 150 mM of boric acid at pH 8.5.…”

Analysis of natural antioxidants by capillary electromigration methods

“…One example is the work by Rodriguez-Delgado et al [57] in which the determination of several flavonoids in wine samples was described. Thus, the use of a high electrolyte concentration buffer along with a medium SDS concentration and the addition of a small proportion of methanol drawn to the most favourable combination [57].…”

“…Thus, the use of a high electrolyte concentration buffer along with a medium SDS concentration and the addition of a small proportion of methanol drawn to the most favourable combination [57]. Honey and other related products like propolis have been also widely studied using both, CZE [58,59] and MEKC [60][61][62].…”