ABSTRACT. The development of meiotic division and associated genetic recombination paved the way for evolutionary changes. However, the secondary and tertiary structure and functional domains of many of the proteins involved in genetic recombination have not been studied in detail. We used the human Dmc1 gene product along with secondary and tertiary domain structures of Escherichia coli RecA protein to help determine the molecular structure and function of maize Dmc1, which is required for synaptonemal complex formation and cell cycle progression. The maize recombinase Dmc1 gene was cloned and characterized, using rice Dmc1 cDNA as an orthologue. The deduced amino acid sequence was used for Cloning of maize meiotic recombinase Dmc1 and its structure elaborating its 3-D structure, and functional analysis was made with the CDD software, showing significant identity of the Dmc1 gene product in Zea mays with that of Homo sapiens. Based on these results, the domains and motives of WalkerA and WalkerB as ATP binding sites, a multimer site (BRC) interface, the putative ssDNA binding L1 and L2 loops, the putative dsDNA binding helix-hairpinhelix, a polymerization motif, the subunit rotation motif, and a small N-terminal domain were proposed for maize recombinase Dmc1.
Introduction: The quality of the relationship between children and parents in the early years of life is one of the most effective factors in the mental growth of the children. A parent being affected by schizophrenia may have very deep effects on the mental growth of the children. This study aimed to compare the behavioral problems in children with one schizophrenic parent and children with healthy parents. Material and methods: This descriptive study was performed in Zahedan, Iran in 2014-2015. The behavioral problems of 60 children with a schizophrenic parent were compared to that of 60 children with healthy parents. The Child Behavior Checklist (CBCL) was used to evaluate the two groups. Results: The comparison of the CBCL score between two groups showed a significant difference (P < 0.005). Moreover, the CBCL score of primary school girls (P = 0.03) and boys (P = 0.04) was significantly different between the two groups. No significant difference was reported between the two groups regarding the CBCL score of teenage girls (P = 0.09) and teenage boys (P = 0.09). Conclusions: Our findings can be regarded as evidence supporting the effect of psychotic disorders, such as schizophrenia in parents on the behavioral problems of children.
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