Callus induction and plant regeneration from an ancient and forgotten crop plant Vicia ervilia L. (WILLD)

Rapid in vitro Multiplication, Callogenesis and Indirect Shoot Regeneration in Ipomoea Mauritiana – A Rare Medicinal Plant in Bangladesh

“…Saggoo and Kaur [12] studied on important medicinal plant Aloe vera L and used 1.0 mg/l BAP and 0.2 mg/l NAA as the regeneration medium for callus. Etedali et al [13] used 1.0 mg/l BAP and 0.2 mg/l NAA in the regeneration medium for an ancient crop plant Vicia ervilia. Narasimhulu and Reddy [14] obtained highest shoot regeneration from callus derived from epicotyl, hypocotyl, leaves and cotyledons in the medium containing 1.0 mg/l BAP and 0.2 mg/l of NAA in Arachis hypogaea L. In our experimental studies rooting was developed from microshoots in half strength MS medium supplemented with different concentrations ( 0.1, 0.2 and 0.5 mg/l) of IBA used alone or in combination with two concentrations (0.5, 1.0 mg/l ) of BAP and Kn.…”