1. When a guinea pig with well developed peritoneal tuberculosis is injected intraperitoneally with about 20 cc. of a heavy suspension of a virulent tubercle bacillus (Culture H37) death occurs within 24 hours or the animal becomes extremely toxic. Such a peritoneal tuberculosis develops in about 1 month after 1 cc. of. a very heavy suspension of Culture H37 has been introduced into the abdominal cavity. If the viscid fluid which is contained within the peritoneal cavity is mixed with saline solution and passed through a Berkefeld filter a bacillus-free filtrate is obtained which induces in normal guinea pigs a certain degree of cutaneous hypersensitiveness to tuberculin. 2. The abdominal organs and the parietal peritoneum, to which masses of leucocytes and tubercle bacilli are adherent, when crushed and extracted with saline solution yield a filtrate which likewise induces a cutaneous hypersensitiveness. 3. The cutaneous hypersensitiveness does not appear before the 7th or 8th day after the filtrate injection and is therefore considered to be the result of an active sensitization of the animal.
Itesumido por el aut'or, Frank Adam McJunkin.El origcn dc las celultzs monoiiue1e:ires fagocitarias de la sangre perif6rica.Tntroducciibn : La fagocitosis c o i m u m f uricibn especializada de ciertas c6lulas : iiaturaleza del carbbn ingerido por fagocitosis bajo condiciories experirneutales; condiciones haj o las cuales tiene lugar la fagocitosis del c s r b h in rivo. Experimentos que deniuestran el origen de 1as cfilulas niononucleares fagocitarias de la sangre, a expensas del endotelio de 10s ~a s o s sanguiricws. Localizaciibn del carbibri ingerido en 10s leiicocitos niononucleares fagocitarios de la sangre y celulas eridoteliales de 10s vasos sanguineos de pcqucfio tamaiio ; mitosis de c4Iul:ts yue conticncn carbbn, en les paredes de 10s 17x50s sariguiriens quc no estan alargAndose; mtividad fagocitica de la.; c6lulxs mieloblisticss ,j6venes; probable origeri de cierto nririiero dc cdulas monoiiurleares fagoritarias de la sangre a expelisas de la t h i c u endotelial de 10s vasos linfjticos C'onclusiones. Alecliant e una inyccciOn intravenosa sirriulthea cle negro c k hurno .v citrsto s6dico en perros y conejos, el carbhn es solamerite ingerido por. ciertas cclulas rriononuclearcs de la saiigre y por 13s c6lulas cntiot elialei de 10s vasos sanguineos pequedos. E K~ la mitosis de las c6lulas endoteliales con carhiri ingcrido, tie lo.; eapilnres dc 10s Grgaiios siblidos (sinusoides del higado) , se encuentrs uns iiueva prueba del origen de dichas celulas sanguineas a expensas del endotelio y de su pcrictrsciOri en el torrente circulatorio. Aunque el 3 por cierito dc ltts cklulas mononucleares que han ingerido carhbn se encuentran en Ia sangre periferica iinas pocas horas despies de la iibn, tales c6lulas no se enpuentran pasadas 24 horas. Este hecho explica perfectaniente el fracas0 de 10s que han trabajado con coloraciones intravi tales para denio5trar la existcricia de c6lulas tefiidas por este m&o lo, cn la sarigre perifhica.
The published reports on the effect of thyroidectomy on neoplastic growth present divergent opinions. Rohdenburg, Bullock and Johnston1 in a series of 29 thyroidectomized rats found at 40 days after inoculation with Flexner-Jobling carcinoma 52 % of "cures" against only 16% of regressions in the control group. Recently Bischoff and Maxwell2 inoculated 13 thyroparathyroidectomized rats with rat carcinoma 256. Based on a comparison with 16 inoculated litter-mate controls they observed "no sign'ificant difference in percentage of takes, rate of tumor growth, or final mortality".Our tumor, a sarcoma, which arose spontaneously in a rat uterus has been transplanted during the past three years into more than lo00 rats. Successfully inoculated tumors in normal rats have not been seen to regress. This is mentioned to indicate that a spontaneous regression of this tumor, if it does occur, 5s uncommon. The operation consists of complete ablation of thyroid and parathyroid glands. All rats were from our colony of Wistar Institute albino stock. All individuals of the litters, or groups, were inoculated bilaterally into groin pockets from the sama tumor and a t the same time.In Table I where the tabulation is by litters the 4 longest postthyroidectomy ones ( 1 , 2, 3, 4) are placed first. Of the 18 successful takes in this group 14 completely regressed. This high percentage (77%) of regressions in a group where the shortest postthyroidectomy period was 112 days is in contrast with 25% of regressions in all successfully inoculated remaining rats with shorter post-operative periods. In both groups it is seen that a first inoculation may fail and a second one succeed. This is true also for normal control animals, and sometimes may result from the accidental introduction of bacteria into the pocket. In the rats with relatively short post-thyroidectomy periods there is an usual tendency for the second inoculation tumors to regress. This may be explained in
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.