Background/aim: Quorum sensing (QS) is a chemical communication process that bacteria use to regulate virulence. Inhibition of QS (antiQS) overcomes the pathogenicity of bacteria. Silver nanoparticles (AgNPs) have been used as antimicrobials against pathogens, but have not been used against QS-mediated bacterial infection. Also, studies have been carried out on the inhibitory effects of propolis based structures on pathogen growth, but no studies have been found on their potential use as QS inhibitor. The present study aims to investigate the synthesis and characterization of silver nanoparticles (AgNPs) reduced with propolis extract (P-AgNPs) and evaluation of their antimicrobial and, for the first time, antiQS activity. Materials and methods: P-AgNPs were synthesized using with different volumes (1, 2.5 and 5 mL) of propolis extract (PE) by biological method via reduction of silver nitrate. Synthesized P-AgNPs were characterized in terms of hydrodynamic, chemical, morphological, physical, and antioxidant properties. Disc diffusion and flask incubation assays were used to evaluate the antimicrobial effect against Gram-negative bacteria (Escherichia coli, Proteus mirabilis, Proteus vulgaris, Salmonella typhimurium, Enterobacter aerogenes, Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus, Streptococcus mutans, Bacillus thuringiensis) and QSregulated biofilm activity against biosensor strain Chromobacterium violaceum CV026. Results: AgNPs were successfully synthesized by biological method via PE. The violacein pigment production based on the QS system was greatly inhibited by the P-AgNPs (inhibition zones: 16.22-21.48 mm and violacein inhibition: 63.16 ± 2.4-75.24 ± 3.5 %) without interfering with the growth of bacteria, which is the first report on the antiQS effect of P-AgNPs. Conclusion: Our results suggest that P-AgNPs may be potentially used to inhibit bacterial physiological processes due to the signal molecules regulates important collective behavior of bacteria. The development of such nontoxic biomaterials may have great potential to evaluate for the new medicinal substance that inhibits the pathogenic biofilms.
Characterization and microbial growth activity of polyvinyl alcohol (PVA) nanofibers (NFs) produced by an electrospinning technique containing different amounts of silver nanoparticles (AgNPs) was investigated. AgNPs were synthesized by chemical reduction of silver nitrate (AgNO 3) (in different weight ratios) in PVA solution followed by electrospinning to produce PVA/AgNPs NFs. The properties of PVA/AgNPs NFs were studied as a function of the silver content. The morphology and distribution of NFs and AgNPs were characterized by transmission and scanning electron microscopy. The size of NFs was 135-160 nm, and the size of AgNPs was 15-27 nm. TEM images clearly showed that spherical AgNPs were homogeneously integrated in PVA NFs. The microbial activity of NFs containing different amounts of AgNPs was examined against both Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Bacillus megaterium and Staphylococcus aureus) bacteria and against Candida albicans. PVA NFs containing AgNPs exhibited increasing inhibitory effects against microbial pathogenic strains with increasing AgNPs amounts. The results suggest that PVA/AgNPs NFs could be used for wound dressing components, protective coatings, biomedical devices, and water purification.
Background/aim: Bone tumor is one of the major causes of tissue bone loss, particularly after performing surgical excision operation to bone lesion that needs to be replaced by biomaterials and ensure a complete filling of tissue-loss spaces. The purpose of our study was to produce a nanofiber-based bone graft scaffold to fill the gaps resulted from bone cancer treatment and also capable of carrying functional molecules that can play a major role in preventing further cancer growth at the targeted bone tissue. Materials and methods: Electrospinning method was used in order to produce nanofibers from different kinds of polymers; Hyaluronic acid (HA), Polyethylene oxide (PEO) and Polyvinyl alcohol (PVA) blended with different concentrations of herbal antibiotic and anti cancer flavonoid molecules called Baicalein (BE). The morphological and chemical structures of scaffold samples were studied using Scanning Electron Microscope (SEM), Fourier Transform Infrared-spectroscopy (FT-IR) and Surface-enhanced Raman spectroscopy (SERS) Analysis. Results: The results showed production of homogenous nanofibers-based scaffold (diameter between 80 nm and 470 nm) that contains the polymers used in the spinning process and the entrapped Baicalein molecules within the nanofiber structure. Conclusion: It was concluded that successful formation of bone tissue mimicking scaffold can be achieved by using Electrospinning method that produces nonwoven nanofibers and at the same time can hold functional anticancer agent such as Baicalein, which may allow using these types of scaffold in bone cancer treatment procedures.
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