Plants have been used for many purposes in different communities. Plants used in alternative medicine since ancient times have been the main material in the treatment of many diseases. In this context, it is very important to determine the biological potential of plants. In our study, total antioxidant status, total oxidant status, oxidative stress index and DPPH free radical scavenging activity of Euphorbia eriophora Boiss. were determined. The aerial parts of the plant were extracted with ethanol in a Soxhlet device. TAS, TOS and OSI values of the plant extract were determined using Rel Assay kits. In addition, the free radical scavenging activity of the plant extract was measured by the DPPH method. As a result of the studies, the TAS value of the plant extract was 5.390±0.227, the TOS value was 20.971±0.348, and the OSI value was 0.390±0.014. The DPPH activity of the plant extract was determined to have an inhibition value of 68.721±1.694% at 2 mg/mL concentration. As a result, in our study, it was determined that E. eriophora has antioxidant potential and can be used as a natural antioxidant agent in this context.

Many different natural materials are used in complementary medicine. Among natural products, herbal materials are used quite a lot. Centaurea rigida Willd. was used as material in this study. The antioxidant and oxidant potential of the plant was determined. The aerial parts of the plant sample were extracted with ethanol in a soxhlet device. Antioxidant and oxidant potentials were measured with Rel Assay kits. As a result of the studies, the total antioxidant value of the plant was determined as 3.522±0.166, the total oxidant value as 15.424±0.281 and the oxidative stress index as 0.440±0.020. It was determined that the plant has antioxidant potential, but its oxidant values are high. As a result, it was determined that C. rigida could be used as a natural antioxidant source.

It is known that plants have been used by humans for a very long time in the treatment of diseases. A wide variety of plant species grown in different regions have been very successful in the treatment of many diseases. In this study, Echium italicum L. plant collected from different regions was used as material. The plant was extracted with ethanol in a soxhlet device. Then, antioxidant and oxidant levels of the plant extract were determined using Rel Assay kits. As a result of the analysis, the TAS values of the plant were determined 5.442±0.240 (Gaziantep), 4.896±0.081 (Hakkari) and 6.056±0.139 (Mersin), TOS values were 21.372±0.747 (Gaziantep), 12.255±0.341 (Hakkari) and 19.107±0.438 (Mersin), OSI values were 0.395±0.027 (Gaziantep), 0.250±0.005 (Hakkari), and 0.316±0.005 (Mersin). It was observed that antioxidant and oxidant values changed as the regions where the plant was collected changed. In addition, it was determined that E. italicum could be a natural antioxidant source.

Plants have been used for centuries to treat various diseases. In this study, Marrubium globosum Montbret & Aucher ex Benth. plant was used as a material. The extract of the plant was extracted with the help of soxhlet device using ethanol, which is a good polar solvent, and Rel Assay kits were used to determine the oxidant and antioxidant levels in the plant extract. Antifungal and antibacterial activities of M. globossum were tested against standard bacteria and fungus strains by agar dilution method. As a result of the analysis, TAS value of plant extract was determined as 7.677±0.231, TOS value as 12.387±0.083 and OSI value as 0.162±0.004. In this context, it has been observed that the plant has an important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 50-200 μg/mL extract concentrations. As a result, it has been determined that M. globossum can be a natural antioxidant and antimicrobial source.

In this study, the antioxidant and antimicrobial potentials of the ethanol extract of the aerial parts of Lepidium spinosum Ard., which is distributed in many regions of the world, were determined. The aerial parts of the plant were dried, powdered and extracted with ethanol. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of the plant extract were determined using Rel Assay Diagnostics kits. Antimicrobial activities of the plant extract were determined against standard bacterial and fungal strains by agar dilution method. As a result of the studies, the TAS value of the plant extract was determined as 4.550±0.132, the TOS value as 12.610±0.221, and the OSI value as 0.277±0.007. In addition, it has been found to be effective at 25-200 µg/mL against standard bacterial strains and 100-200 µg/mL concentrations against fungal strains. As a result, it was determined that L. spinosum used in our study could be a natural antioxidant and antimicrobial source. In this context, it is thought that natural material may be used in pharmacological designs.

Many people in different parts of the world benefit from alternative medicine in the treatment and prevention of diseases. Plants are among the important natural materials used in alternative medicine. In this study, the antioxidant and antimicrobial potential of Datura stramonium L. was determined. Ethanol extracts of the plant's flower parts were obtained in the soxhlet device. The antioxidant and oxidant potential of the plant extract was determined using Rel Assay TAS and TOS kits. Antimicrobial activity was tested by the agar dilution method. The TAS value of the plant extract was 7.559±0.224 mmol/L, the TOS value was 10.711±0.243 μmol/L, and the OSI value was 0.142±0.002. It was determined that the plant extract was effective against bacteria and fungus strains at 100-400 μg/mL concentration. As a result, it was determined in our study that D. stramonium can be a natural antioxidant and antimicrobial source.

Many natural products are used in complementary medicine. Plants are widely used among these natural products. In this study, it was aimed to determine the total phenolic and flavonoid contents, total antioxidant status and antimicrobial activity of Hesperis pendula DC. In this context, the above-ground parts of the plant were extracted with ethanol and methanol. The total antioxidant level of the plant was determined using Rel Assay Diagnostics kits (Megatıp/Türkiye). The total phenolic content was assessed using the Folin-Ciocalteu reagent. Aluminum chloride assay was used to estimate the total flavonoid content. Antimicrobial activity was tested against bacterial and fungal strains by agar dilution method. As a result of the studies, it was observed that the ethanol extract of the plant had higher TAS (Total antioxidant status) (5.707±0.194 mmol/L), TOS (Total oxidant status) (21.646±0.239 µmol/L) and OSI (Oxidative stress index) (0.380±0.017) values. Total phenolic content was higher in ethanol extract (116.78±2.51 mg/g) while total flavonoid content was higher in methanol extract (93.64±2.16 mg/g). It was observed that the ethanol and methanol extracts of the plant inhibited the growth of bacteria at  100-200 µg/mL concentrations. It was determined that ethanol extract inhibited the growth of fungi at 200 µg/mL concentration and methanol extract at 200-400 µg/mL concentrations. In this context, it was determined that H. pendula could be a natural antioxidant and antimicrobial source.

Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status (TOS) and oxidative stress index (OSI) and total antioxidant status (TAS) of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected. Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line (A549) was used to find out the antiproliferative effect by MTT assay. Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.752±0.139, the TOS capacitiy as 7.886±0.224 and the OSI capacitiy as 0.117±0.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μg/ml intensiy and against fungi at 100-200 μg/ml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.