This study investigates the effects of different temperatures on the total carbohydrate, lipid and protein amounts of Acanthoscelides obtectus Say, which is a common cereal pest. Studies have been carried out under laboratory conditions at 20 +/- 2 degrees C, 30 +/- 2 degrees C and 60 +/- 5% relative humidity. No specific photoperiodic regimen has been used throughout the study. Total carbohydrate, protein and lipid amounts for females at 20 degrees C were 61.74, 35.77 and 83.79 microg/individual, respectively, whereas the amounts for males were 34.94, 29.53 and 57.98 microg/individual, respectively. At 30 degrees C, total carbohydrate, protein and lipid amounts for females were 92.00, 42.18 and 83.26 microg/individual, respectively. The amounts at the same temperature for males were 43.34, 34.08 and 52.19 microg/individual, respectively. In both sexes, total carbohydrate and protein amounts at 30 degrees C were higher than those at 20 degrees C whereas this was not true for total lipid amounts.
Low temperatures affect many life history traits of insects. The purpose of this study was to assess the effects of duration of cold exposure on the length of Tenebrio molitor’s pupal period, proportion of adult emergence, adult weight and proportion of deformed adults. The trials were conducted in a laboratory in constant conditions with wheat flour and whole-wheat flour meal were used as food. Cold-exposed groups were achieved by exposing pupae taken on the first day after pupation for 1, 3, 5, 10 and 15 days at +4 °C. The results showed that as the period of cold increased, pupal period extended and proportion of deformed adults increased whereas there were no or less straightforward effects on adult weights and proportion of emerged adults, respectively
Denemelerde değirmen güvesi Ephestia kuehniella kullanıldı. Denemeler 28°C, 65±5 sıcaklık ve nem şartlarında ve devamlı karanlık koşullarda yapıldı. Besin olarak buğday unu, mısır unu, rüşeym katkılı un ve rüşeym kullanıldı. Belirtilen besinlerde yetiştirilen larva, pup ve ergin dişi ile erkeklerden 3'er tekrar olmak üzere 40 birey alındı. Toplamda her bir deneme için 120 birey kullanıldı. Toplam lipit ve toplam yağ asitlerinin özütlenmesinde Folch vd. [8]'nin geliştirdikleri yöntem uygulandı. Denemeler sonucunda en ağır larva mısır ununda, pup rüşeym katkılı unda, dişi buğday ununda, erkek ise rüşeymde yetiştirilenlerde bulundu. Total lipit larvada en fazla buğday ununda, pup, dişi ve erkekte mısır ununda yetiştirilenlerde tespit edildi. Total yağ asidi ise larva ve pupta en fazla mısır ununda, dişi ve erkekte ise rüşeym katkılı unda yetiştirilenlerde bulundu. Değerler arasındaki farklar genellikle istatistiksel olarak anlamlı değildi.
The ever-increasing world population indicates that it is inevitable to consider insects such as Tenebrio molitor, which are used as live feed and even human food in some countries, as an alternative food source. Especially T. molitor larvae are a source of food with high nutritive value for including high lipid and protein. This study seeks answer to the question "will the insect continue to keep its lipid sources during the periods in which it is kept in the cold, or will it continue to use its energy sources since the physiological adaptations it develops are not enough?" The main material of this study was T. molitor cultures. Flour: wheat flour (250 g: 250 g) in a ratio of 1:1 was used as food. 25 g wheat germ and 5 g dry yeast was put in it. Larvae at stages 13-15 th were grouped as control and trial groups and kept for 5, 10, 15 and 20 days at specified temperatures. The weights, total lipid content and percentages of the larvae whose storage period was over were determined. This study evaluates the total lipid amount and percentages of T. molitor larvae stored in refrigerator for different periods. Total lipid amount and percentages of the larvae stored in the cold for 5, 10 and 15 days were found to be higher when compared with the control group. A tendency to decrease was observed in larvae kept for 20 days. As a result, it is recommended for producers not to keep in the refrigerator for more than 15 days. Otherwise, it should be considered that there may be a decrease in important energy and food sources.
Effects of cold storage on the developmental biology of Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) at different larval stages Soğukta depolamanın farklı dönemlerdeki Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) larvalarının gelişimsel biyolojisine etkileri
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