ObjectiveTo examine porcine acellular dermal matrix (ADM) as a xenogenic dermal substitute in a rat model. Summary Background DataAcellular dermal matrix has been used in the treatment of fullthickness skin injuries as an allogenic dermal substitute providing a stable wound base in human and animal studies. MethodsXenogenic and allogenic ADMs were produced by treating porcine or rat skin with Dispase and Triton X-100. Full-thickness skin defects (225 mm 2 ) were created on the dorsum of rats (n ϭ 29), porcine or rat ADMs were implanted in them, and these were overlain with ultrathin split-thickness skin grafts (STSGs). In two adjacent wounds, 0.005-or 0.017-inch-thick autografts were implanted. In other experiments, the antimicrobial agent used during ADM processing (azide or a mixture of antibiotics) and the orientation of the implanted ADM (papillary or reticular side of ADM facing the STSG) were studied. Grafts were evaluated grossly and histologically for 30 days after surgery. ResultsSignificant wound contraction was seen at 14, 20, and 30 days after surgery in wounds receiving xenogenic ADM, allogenic ADM, and thin STSGs. Contraction of wounds containing xenogenic ADM was significantly greater than that of wounds containing allogenic ADM at 30 days after surgery. Graft take was poor in wounds containing xenogenic ADM and moderately good in those containing allogenic ADM. Wound healing was not significantly affected by the antimicrobial agent used during ADM preparation or by the ADM orientation. ConclusionDispase-Triton-treated allogenic ADM was useful as a dermal substitute in full-thickness skin defects, but healing with xenogenic ADM was poor.In extensive deep burns and other full-thickness skin wounds, permanent replacement of lost skin remains a major challenge. Several methods of wound closure are in use, and each has its own advantages and disadvantages. Porcine skin and preserved cadaver skin are used for temporary wound coverage, but 1 to 2 weeks after grafting, these tissues undergo immune-mediated rejection.1,2 Permanent wound coverage is usually accomplished using meshed, split-thickness autografts harvested from undamaged regions of skin. Extensively burned patients have limited donor sites, so thin split-thickness autografts are harvested repeatedly from the same sites. This results in substantial donor-site problems resulting from pain, infection, scarring, and sometimes keloid formation.3 Very thin (0.005-inchthick) meshed autografts can be used, but the lack of a sufficient dermal bed often results in extensive wound contraction at the recipient site. 4 The harvest of thicker splitthickness skin grafts (STSGs) reduces this contraction problem but causes increased problems at donor sites. Alternatively, cultured epidermal autografts expanded using cell culture methods can be used for wound coverage, but this technique is expensive, and cultured epidermal autografts often fail to survive or result in poor-quality wound healing.5-8 Many of these difficulties with STSGs and cul-
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.