Spirulina sp adalah salah satu pakan alami yang umum digunakan dalam kegiatan pemeliharaan larva ikan dan udang. Penambahan pupuk kascing sebagai sumber nutrien diduga dapat digunakan untuk meningkatkan kelimpahan sel Spirulina sp. Adapun manfaat dari penelitian ini diharapkan dapat menjadi sumber informasi untuk masyarakat dan instansi terkait dalam membudidayakan pakan alami Spirulina sp. Penelitian ini telah dilaksanakan pada tanggal 25 Februari-16 Maret 2017 di Balai Perikanan Budidaya Air Payau Ujong Batee, Kecamatan Mesjid Raya, Kabupaten Aceh Besar, Provinsi Aceh. Metode penelitian yang digunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) non faktorial yang terdiri dari 4 perlakuan dan 3 ulangan. Adapun dalam penelitian ini adalah perlakuan A (media kultur kascing 0,7 gr/l), perlakuan B (media kultur kascing 0,8gr/l), perlakuan C (media kultur kascing 0,9gr/l), perlakuan D (kontrol menggunakan pupuk UREA, ZA, TSP, Fecl, EDTA dan vitamin B12). Hasil penelitian menunjukkan bahwa penggunaan pupuk kascing untuk meningkatkan kelimpahan sel Spirulina sp memberikan pengaruh sangat berbeda nyata dengan F Hitung (35,75) > F Tabel0.01(7.59) terhadap kelimpahan rata-rata sel Spirulina sp akan tetapi tidak memberikan pengaruh berbeda nyata terhadap puncak populasi Spirulina sp dengan F hitung (2.59) < F Tabel0.05 (4.07) terhadap puncak populasi Spirulina sp.Kata Kunci: Spirulina sp, Pupuk Kascing, Kelimpahan Harian,Puncak Populasi
Floating net cage activities can cause water quality to decrease. The feed remains and the metabolism of the fish from these activities produces nitrogen and phosphorus . The objective of this study is to obtain the calculation model and the amount of phosphorus released into the waters of Laut Tawar Lake as a result of floating cage activities. The resulting formula was Pw = (F x [P]p) – ((It – Io) x [P]i), where their variables were given feed (F), phosphorus concentration of feed ([P]p), the final weight of fish (It), the initial weight of fish (Io), and phosphorus concentration in fishes ([P]i). It can be used to predict the increase in phosphorus waste based on the increase in floating net cage area. The weight of tilapia increases exponentially with the day of cultivation in the equation Y = 12.70e0.02x and the goldfish followed the formula Y = 2.28e0.03x. Phosphorus in tilapia varies from 1.58% to 2.23% with an average of 1.97%. Goldfish contain 1.19 - 2.02% phosphorus, with an average of 1.52%. Goldfish growth was not optimal due to inadequate feeding, so without excessive feeding and did not generate phosphorus waste. The cultivation of tilapia releases 0.09 Kg P/m2 of phosphorus waste from floating cages .
This study aimed to investigate the efficiency of fish silage mixed in compounded diets on growth and survival rate of grouper (Epinephelus coioides). Grouper (2,34±0,24 cm, and 4,17±1,82 g) were divided into four groups and cultivated in 1x1x1,5 m floating net cages. Each group fed with compounded diets containing 0, 1, 3 and 5 g/kg fish silage diet twice daily. Fish were sampled for growth performances and feed efficiency at 14 days interval for 10 weeks. Results indicated that compounded diets at 1 and 3 g/kg silage affected the growth rate. Total length, specific growth rate and weight gain significantly increased in fish received 1 and 3 g/kg silage diets. The feed intake seen to be highest in groups administered 3 g/kg silage, whereas the best feed conversion ratio was found in fish fed 1 g/kg silage. Thus, this study indicated that using fish silage in compounded diets may affect feed efficiency and growth performances of grouper juveniles.Keywords:Artificial dietFeedingFish silageGrouperNet cages
The trophic status of Laut Tawar Lake was increasingly leading to an alarming level of degradation. Efforts to anticipate an increase in trophic status require scientific knowledge of the trophic phenomena of the waters empirically. This research examines the trophic status parameters to produce a model of the trophic status of Laut Tawar Lake. The proposed model was a modification of Carlson's Trophic State Index Method. Analysis of the relationship examined the interaction of water clarity (SD) with chlorophyll-a (Chl), Total Suspended Solid, and Total Dissolved Solids, and the interaction of chlorophyll-a with nutrient concentration (TP, TN). Data were analyzed descriptively quantitatively, correlation, and regression. The results showed that the waters of Laut Tawar Lake contain phosphate (TP) 34 µg/L, Total Nitrogen 687 µg/L, chlorophyll-a 10 µg/L, Total Suspended Solid 47 mg/L, Total Dissolved Solid 84 mg/L, and water clarity 4.0 m. The regression test showed that the abundance of phytoplankton (Chl) was affected by nutrient phosphate with the empirical model equation Chl = 0.565 TP – 9.161. Nitrogen nutrients did not partially affect the concentration of chlorophyll-a in the waters of Laut Tawar Lake. Water clarity is influenced by chlorophyll-a and TSS, where TDS has no effect partially. The empirical model obtained is Ln SD = 1.757 – 0.013 Chl – 0.008 TSS. Modification of Carlson's TSI by substituting the two equations obtained the equation TSIdlt = 13.46 + 8.08 ln TP + 0.04 TP + 0.04 TSS. This model simulation gives an estimation rate of 91.06%.
<p>Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of <em>Vibrio harveyi</em>. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of <em>V. harveyi</em> was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of <em>V. harveyi</em>, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic). Number of <em>V. harveyi </em>in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture. It demonstrated possible inhibition of probiotic bacteria on <em>V. harveyi</em> through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to <em>Pseudoalteromonas piscicida</em>, whereas SKT-b and Ua were similar to <em>Vibrio alginolyticus</em>.</p> <p>Keywords: probiotic bacteria, inhibitory mechanism, <em>V. harveyi</em>, tiger shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan<em> V. harveyi </em>secara <em>in vitro</em>. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (Rf<sup>R</sup>) melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu. Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan <em>V. harveyi</em> diamati melalui pemberian secara bersamaan antara bakteri probiotik dan <em>V. harveyi</em> tersebut dalam air pemeliharaan larva udang. Jumlah sel bakteri probiotik,<em> V. harveyi</em> dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung. Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik). Jumlah sel <em>V. harveyi</em> pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap <em>V. harveyi</em> yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies <em>Pseudoalteromonas piscicida, </em>sedangkan SKT-b dan Ua termasuk spesies <em>Vibrio alginolyticus</em>. </p> <p>Kata kunci: bakteri probiotik, mekanisme penghambatan, <em>V. harveyi</em>, udang windu</p>
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