Background:The outbreak of COVID-19 manifests in peoples’ mental health and psychological dispositions and may also result to acute distress, depression, anxiety and post-traumatic stress disorder (PTSD) on victims. The objective of this study was to support business educators’ and students’ mental health against coronavirus trauma using trauma-focused cognitive behavioral therapy (TF-CBT).Methods:The participants (n = 74 – educators and students) who indicated PTSD symptoms based on the self-reporting questionnaire they filled, were randomly assigned to treatment and waitlist control groups. The TF-CBT manual was used for the intervention. The intervention was a 14-week treatment with 2 weeks follow-up meeting. Using repeated measures of Analysis of Variance, we determined the level of improvement of each participant in the treatment group against Coronavirus trauma at the end of the intervention.Results:The study established that gender has no significant difference in determining the effects of Coronavirus trauma on participants. Through intervention, the PTSDs and depression arising from Coronavirus event were significantly reduced in the treatment group. Again, there was a significant improvement in the participants’ general mental health at the end of the intervention. Hence, we established the implication for research and practice in line with the outcome of the study. The study advocates that TF-CBT should be employed by educational institutions in Nigeria to cushion the traumatic effects of coronavirus and future disasters on both workers and students.Conclusion:We conclude that mental health problems remain a serious challenge among business educators and students in this COVID-19 pandemic era. The study established that low educational qualifications, PTSD symptoms and negative coping strategies are the factors aggravating the general low mental health of the study participants. Finally, we advocate that the educational institutions in Nigeria should adopt TF-CBT interventions to support staff and students’ mental health against adverse effects of COVID-19.

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Aims: The aims of the study were to evaluate the multidrug resistance profile and mechanisms of carbapenem resistance in Pseudomonas aeruginosa clinical isolates using phenotypic and genotypic methods. Study Design: A descriptive laboratory based study. Place and Duration of Study: Microbiology Laboratory, Ondo State University of Science and Technology, Okitipupa, and Biotechnology Laboratory, Ladoke Akintola University of Technology, Osogbo, Nigeria, between June 2017 and November 2018. Methodology: Ten P. aeruginosa isolates were recovered from patients at Lagos University Teaching Hospital, and susceptibilities to imipenem (10 µg), meropenem (10 µg) and a panel of antibiotics were performed by the disk diffusion method. Genotypic methods including Polymerase Chain Reactions (PCR) and agarose gel electrophoresis were carried out according to established protocols. oprD and blaIMP gene primers were used for the PCR amplification. Results: Fifty percent (50%) of the isolates showed multiple drug resistance. Four isolates (40%) were carbapenem resistant (CR). oprD gene was detectedin 90% (9/10) of the isolates. 75% (3/4) of CR strains were among the strains showing oprD gene. 25% (1/4) CR strain (PA1421) was oprD negative. Loss or mutation of oprD gene seems to be the mechanism of carbapenem resistance in strain PA1421. Conclusion: Loss or mutation of oprD gene was identified in this study as a mechanism of carbapenem resistance. oprD gene encodes the outer membrane protein (OprD) porin in P. aeruginosa whose deficiency confers resistance to carbapenems, especially imipenem. Surveillance of the antimicrobial susceptibility patterns of P. aeruginosa is of critical importance in understanding new and emerging resistance trends, reviewing antibiotic policies and informing therapeutic options.

Aims: The present study examined the influence of age, health care and hygienic habits on the prevalence of Candida species in the human oral cavity and genitourinary tract. Study Design: The study was a cross sectional study. Place and Duration of Study: Department of Microbiology, University of Nigeria, Nsukka and Bishop Shanahan Hospital, Nsukka, between March 2006 and February 2007. Methodology: Oral and genitourinary samples were collected from 218 individuals (45 males, 173 females) within the ages of 12 and 67 years. Ninety-four of these volunteers responded to the questionnaire on health care and hygienic habits. The clinical specimen collected were cultured for the presence of Candida species. The data obtained were statistically presented as means and percentages.    Results: Out of 298 samples collected, 61/154 oral (19 males, 42 females) and 53/144 genitourinary (0 male, 53 females) samples yielded growth of Candida species. There was no significant difference in the prevalence of Candida species between subjects who use toothpaste and those who use chewing stick for oral hygiene (P=0.93). Respondents who douched were more colonized with Candida 26(39.39%) than those who did not (0%). Species of Candida were significantly associated with the textile material of the undergarment (p = 0.044). Age significantly influenced the prevalence of Candida species in the oral cavity (p < 0.05) but not in the genitourinary tract (p = 0.612). Conclusion: The study recommends good personal hygiene and health care habits to reduce proliferation of Candida species. Aims: The present study examined the influence of age, health care and hygienic habits on the prevalence of Candida species in the human oral cavity and genitourinary tract. Study Design: The study was a cross sectional study. Place and Duration of Study: Department of Microbiology, University of Nigeria, Nsukka and Bishop Shanahan Hospital, Nsukka, between March 2006 and February 2007. Methodology: Oral and genitourinary samples were collected from 218 individuals (45 males, 173 females) within the ages of 12 and 67 years. Ninety-four of these volunteers responded to the questionnaire on health care and hygienic habits. The clinical specimen collected were cultured for the presence of Candida species. The data obtained were statistically presented as means and percentages.    Results: Out of 298 samples collected, 61/154 oral (19 males, 42 females) and 53/144 genitourinary (0 male, 53 females) samples yielded growth of Candida species. There was no significant difference in the prevalence of Candida species between subjects who use toothpaste and those who use chewing stick for oral hygiene (P=0.93). Respondents who douched were more colonized with Candida 26(39.39%) than those who did not (0%). Species of Candida were significantly associated with the textile material of the undergarment (p = 0.044). Age significantly influenced the prevalence of Candida species in the oral cavity (p < 0.05) but not in the genitourinary tract (p = 0.612). Conclusion: The study recommends good personal hygiene and health care habits to reduce proliferation of Candida species. Aims: The present study examined the influence of age, health care and hygienic habits on the prevalence of Candida species in the human oral cavity and genitourinary tract. Study Design: The study was a cross sectional study. Place and Duration of Study: Department of Microbiology, University of Nigeria, Nsukka and Bishop Shanahan Hospital, Nsukka, between March 2006 and February 2007. Methodology: Oral and genitourinary samples were collected from 218 individuals (45 males, 173 females) within the ages of 12 and 67 years. Ninety-four of these volunteers responded to the questionnaire on health care and hygienic habits. The clinical specimen collected were cultured for the presence of Candida species. The data obtained were statistically presented as means and percentages.    Results: Out of 298 samples collected, 61/154 oral (19 males, 42 females) and 53/144 genitourinary (0 male, 53 females) samples yielded growth of Candida species. There was no significant difference in the prevalence of Candida species between subjects who use toothpaste and those who use chewing stick for oral hygiene (P=0.93). Respondents who douched were more colonized with Candida 26(39.39%) than those who did not (0%). Species of Candida were significantly associated with the textile material of the undergarment (p = 0.044). Age significantly influenced the prevalence of Candida species in the oral cavity (p < 0.05) but not in the genitourinary tract (p = 0.612). Conclusion: The study recommends good personal hygiene and health care habits to reduce proliferation of Candida species.