The growth of normal cells is arrested when they come in contact with each other, a process known as contact inhibition. Contact inhibition is lost during tumorigenesis, resulting in uncontrolled cell growth. Here, we investigated the role of the tetraspanin transmembrane 4 superfamily member 5 (TM4SF5) in contact inhibition and tumorigenesis. We found that TM4SF5 was overexpressed in human hepatocarcinoma tissue. TM4SF5 expression in clinical samples and in human hepatocellular carcinoma cell lines correlated with enhanced p27 Kip1 expression and cytosolic stabilization as well as morphological elongation mediated by RhoA inactivation. These TM4SF5-mediated effects resulted in epithelial-mesenchymal transition (EMT) via loss of E-cadherin expression. The consequence of this was aberrant cell growth, as assessed by S-phase transition in confluent conditions, anchorage-independent growth, and tumor formation in nude mice. The TM4SF5-mediated effects were abolished by suppressing the expression of either TM4SF5 or cytosolic p27 Kip1 , as well as by reconstituting the expression of E-cadherin. Our observations have revealed a role for TM4SF5 in causing uncontrolled growth of human hepatocarcinoma cells through EMT.
A facile pathway to furan derivatives from lignocellulosic biomass via 5-acetoxymethylfurfural (AMF) was developed. AMF possesses advantageous properties due to its less-hydrophilic acetoxymethyl group relative to the hydroxymethyl group of 5-hydroxymethylfurfural (HMF). The hydrophobicity and chemical stability of AMF allowed practical isolation and purification to afford a highly pure product of up to 99.9 %. AMF was produced in good to excellent yields under mild conditions from 5-chloromethylfurfural (CMF) and alkylammonium acetates, both of which could be obtained directly from lignocellulosic biomass. Heterogeneous reactions with polymer-supported alkylammonium acetates were also established; this showed the feasibility of a continuous process for this pathway. AMF could be transformed into various promising furanic compounds, such as 2,5-furandicarboxylic acid (FDCA), 2,5-furandimethanol (FDM), and 5-hydroxymethyl-2-furanoic acid (HFA), in high yields.
SummaryPKC␦ and cofilin activation affects peripheral actin reorganization and cell-cell contact in cells expressing integrin ␣5 but not its tailless mutant
We investigated the effects of the well protection layer (WPL) and electron reservoir layer (ERL) on the emission properties of InGaN/GaN green multiple quantum wells (MQWs). In order to increase their emission wavelength by preventing the volatile InGaN well, a thin GaN WPL was coated subsequently on each well layer at the same temperature before ramping-up the temperature to grow the GaN barrier. It was found that the WPL directly influenced the indium content and optical properties of the MQW. The indium content was in fact increased, as was evident from the x-ray diffraction and photoluminescence experiments. Then, to explore the possibility of enhancing the quantum efficiency by increasing the electron capture rate, a superlattice ERL composed of ten pairs of InGaN/GaN was embedded between the MQW and n-GaN. The electroluminescence intensity of the green light emitting diode with the ERL was up to three times higher than that of the diode without the ERL. These results imply that the carrier capture by the MQW is significantly improved by the additional superlattice ERL, which consequently leads to the enhancement of the quantum efficiency.
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