The purpose of this study was to compare repetition performance and rating of perceived exertion (RPE) with 1-, 3-, or 5-minute rest intervals between sets of multi and single-joint resistance exercises. Fifteen resistance trained men (23.6 ± 2.64 years, 76.46 ± 7.53 kg, 177 ± 6.98 cm, bench press [BP] relative strength: 1.53 ± 0.25 kg·kg(-1) body mass) completed 12 sessions (4 exercises × 3 rest intervals), with each session involving 5 sets with 10 repetition maximum loads for the free weight BP, machine leg press (LP), machine chest fly (MCF), and machine leg extension (LE) exercises with 1-, 3-, 5-minute rest intervals between sets. The results indicated significantly greater BP repetitions with 3 or 5 minutes vs. 1 minute between sets (p ≤ 0.05); no significant difference was evident between the 3- and 5-minute rest conditions. For the other exercises (i.e., LP, MCF, and LE), significant differences were evident between all rest conditions (1 < 3 < 5; p ≤ 0.05). For all exercises, consistent declines in repetition performance (relative to the first set) were observed for all rest conditions, starting with the second set for the 1-minute condition and the third set for the 3- and 5-minute conditions. Furthermore, significant increases in RPE were evident over successive sets for both the multi and single-joint exercises, with significantly greater values for the 1-minute condition. In conclusion, both multi and single-joint exercises exhibited similar repetition performance patterns and RPE, independent of the rest interval length between sets.

The aim of this study was to investigate the acute effects of different interset rest intervals on performance of single- and multijoint exercises with near-maximal loads. Fifteen trained men (26.40 ± 4.94 years, 79.00 ± 7.10 kg, 176.6 ± 6.06 cm, 11.80 ± 2.47% body fat, and bench press relative strength: 1.26 ± 0.19 kg·kg of body mass) performed eight sessions (2 exercises × 4 interset rest intervals); each consisting of 5 sets with a 3RM load. The exercises tested were the machine chest fly (MCF) for the single-joint exercise and the barbell bench press (BP) for the multi-joint exercise with 1, 2, 3, and 5 minutes of rest between sets. The results indicated that for the MCF, significantly higher total number of repetitions were completed for the 2- (12.60 ± 2.35 reps; p = 0.027), 3- (13.66 ± 1.84 reps; p = 0.001), and 5-minute (12.93 ± 2.25 reps; p = 0.001) vs. the 1-minute (10.33 ± 2.60 reps) protocol. For the BP, a significantly higher total number of repetitions were completed for 3- (11.66 ± 2.79 reps; p = 0.002) and 5-minute (12.93 ± 2.25 reps; p = 0.001) vs. the 1-minute protocol (7.60 ± 3.52 reps). In addition, subjects completed significantly higher total number of repetitions for the 5-minute (12.93 ± 2.25 reps; p = 0.016) vs. 2-minute (9.53 ± 3.11 reps) protocol. Both exercises presented similar and progressive reductions in repetition performance for all rest protocols along the 5 sets, starting as soon as the second set for the shorter 1-minute rest protocol. In conclusion, to maintain the best consistency in repetition performance, rest intervals of 2 minutes between sets are sufficient for the MCF and 3-5 minutes for the BP. Thus, it appears that longer acute recovery time is needed for a multijoint (core) exercise such as the BP vs. a single-joint (assistance) exercise such as the MCF.

The purpose of this study was to compare different rest intervals between sets on repetition consistency and ratings of perceived exertion (RPE) during consecutive bench press sets with an absolute 3RM (3 repetition maximum) load. Sixteen trained men (23.75 ± 4.21 years; 74.63 ± 5.36 kg; 175 ± 4.64 cm; bench press relative strength: 1.44 ± 0.19 kg/kg of body mass) attended 4 randomly ordered sessions during which 5 consecutive sets of the bench press were performed with an absolute 3RM load and 1, 2, 3, or 5 minutes of rest interval between sets. The results indicated that significantly greater bench press repetitions were completed with 2, 3, and 5 minutes vs. 1-minute rest between sets (p ≤ 0.05); no significant differences were noted between the 2, 3, and 5 minutes rest conditions. For the 1-minute rest condition, performance reductions (relative to the first set) were observed commencing with the second set; whereas for the other conditions (2, 3, and 5 minutes rest), performance reductions were not evident until the third and fourth sets. The RPE values before each of the successive sets were significantly greater, commencing with the second set for the 1-minute vs. the 3 and 5 minutes rest conditions. Significant increases were also evident in RPE immediately after each set between the 1 and 5 minutes rest conditions from the second through fifth sets. These findings indicate that when utilizing an absolute 3RM load for the bench press, practitioners may prescribe a time-efficient minimum of 2 minutes rest between sets without significant impairments in repetition performance. However, lower perceived exertion levels may necessitate prescription of a minimum of 3 minutes rest between sets.

Basal protein turnover, which largely relies on the degradation of ubiquitinated substrates, is instrumental for maintenance of muscle mass and function. However, the regulation of ubiquitinated protein degradation in healthy, nonatrophying skeletal muscle is still evolving, and potential tissue-specific modulators remain unknown. Using an unbiased expression analysis of 34 putative autophagy genes across mouse tissues, we identified unc-51 like autophagy activating kinase (Ulk)2, a homolog of the yeast autophagy related protein 1, as particularly enriched in skeletal muscle. Subsequent experiments revealed accumulations of insoluble ubiquitinated protein aggregates associated with the adaptors sequestosome 1 (SQSTM1, also known as p62) and next to breast cancer type 1 susceptibility protein gene 1 protein (NBR1) in adult muscles with ULK2 deficiency. ULK2 deficiency also led to impaired muscle force and caused myofiber atrophy and degeneration. These features were not observed in muscles with deficiency of the ULK2 paralog, ULK1. Furthermore, short-term ULK2 deficiency did not impair autophagy initiation, autophagosome to lysosome fusion, or protease activities of the lysosome and proteasome. Altogether, our results indicate that skeletal muscle ULK2 has a unique role in basal selective protein degradation by stimulating the recognition and proteolytic sequestration of insoluble ubiquitinated protein aggregates associated with p62 and NBR1. These findings have potential implications for conditions of poor protein homeostasis in muscles as observed in several myopathies and aging.—Fuqua, J. D., Mere, C. P., Kronemberger, A., Blomme, J., Bae, D., Turner, K. D., Harris, M. P., Scudese, E., Edwards, M., Ebert, S. M., de Sousa, L. G. O., Bodine, S. C., Yang, L., Adams, C. M., Lira, V. A. ULK2 is essential for degradation of ubiquitinated protein aggregates and homeostasis in skeletal muscle.