Acute kidney injury (AKI) is a common complication in cirrhotic patients. Serum creatinine is a poor biomarker for detection of renal impairment in cirrhotic patients. This study aimed to evaluate urinary neutrophil gelatinase-associated lipocalin (NGAL) and urinary interleukin-18 (IL-18) as early biomarkers of acute kidney injury in cirrhotic patients. 160 patients with cirrhosis admitted to the Liver Units at Zagazig University Hospitals were classified into three groups: (I) nonascitic patients, (II) ascitic patients without renal impairment, and (III) ascitic patients with renal impairment. Patients with renal impairment were further divided into four subgroups: [A] prerenal azotemia, [B] chronic kidney disease (CKD), [C] hepatorenal syndrome (HRS), and [D] acute tubular necrosis (ATN). Significant elevation of both urinary NGAL and urinary IL-18 in cirrhotic patients with renal impairment especially in patients with ATN was observed. Urinary NGAL and urinary IL-18 have the ability to differentiate between AKI types in patients with cirrhosis. This could improve risk stratification for patients admitted to the hospital with cirrhosis, perhaps leading to early ICU admission, transplant evaluation, and prompt initiation of HRS therapy and early management of AKI.

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Fish such as tilapia (Oreochromis niloticus) and catfish (Clarias gariepinus) is an important source of high biological value animal protein. Fish can be exposed to antimicrobials in intensive aquaculture systems or exposed to remnants of the antimicrobials released to various water bodies via drainage systems. This study aimed at screening for antimicrobial residues in two major fish species commonly consumed in Egypt, namely, tilapia, and catfish, either in wild or cultured fish using a microbial inhibition assay. Besides, quantitative estimation of tetracycline (oxytetracycline and doxycycline) residues in the edible fish muscles was carried out using the solid phase extraction (SPE) technique and high-performance liquid chromatography with photodiode-array detection (HPLC-PAD). In addition, the effects of different cooking methods (pan-frying, grilling, and microwaving) on oxytetracycline and doxycycline residues in tilapia and catfish were investigated. The microbiological inhibition assay revealed that 2, 24, 18, and 32% of the examined wild tilapia, cultured tilapia, wild catfish, and cultured catfish, respectively, contained antibiotic residues. Cultured tilapia, wild catfish, and cultured catfish had mean concentrations of oxytetracycline residues of 0.147 ± 0.067, 0.106 ± 0.046, and 0.313 ± 0.044 µg/g. 3 (6%), 4 (8%), and 9 (18%) of the sampled cultured tilapia, wild catfish, and cultured catfish exceeded the established maximum permissible limits (MPL) of tetracycline (0.100 ng/g). The mean concentrations of doxycycline residues were 0.276 ± 0.045, 0.026 ± 0.004, and 0.070 ± 0.010 µg/g in cultured tilapia, wild catfish, and cultured catfish, respectively, with 2 (4%) of the cultured tilapia exceeding the MPL of doxycycline. Consumption of fish with high residual levels of tetracyclines might pose potential health risks to consumers. In an experimental trial, heat treatment of tilapia and catfish could significantly reduce both oxytetracycline and doxycycline residues, particularly grilling had the highest reduction rates.

Tetracycline is one of the most important groups of antibiotics that have harmful effects on the consumers, therefore the public health safety against its residues represents a significant issue. This study aimed to estimate the effect of tetracycline hydrochloride on some hematological parameters, kidneys function tests as well as liver and breast muscle enzymes with special reference to the supposed withdrawal time of this drug in different rabbits' tissues (kidney, liver and muscles), following oral dose of tetracycline using High Performance Liquid Chromatography. Tetracycline was administrated to eighteen rabbits directly into the stomach at a dose of 35 mg/kg BW once daily for five successive days. Samples were collected on the 1 st , 3 rd , 7 th , 14 th , 21 st and 28 th days after the last oral dose. The results revealed that, tetracycline caused a significant increase in the uric acid, urea, creatinine, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK) and lactate dehydrogenase (LDH) activities with no significant changes in the hematological parameters when compared with the control group. The residues remained in the liver and kidney for 7 days, while in muscles for 3 days only after the last oral dose of the drug. In conclusion, the disturbances in the biological parameters occurred by tetracycline administration in rabbits was transient and returned to normal after 7 days of last treatment. The withdrawal time of tetracycline was 14 days from the rabbit's tissues.

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Background Hepatocellular carcinoma (HCC) is a prevalent malignancy worldwide. Vitamin D receptor (VDR) gene polymorphisms were linked to different cancers. This study was carried out to assess the possible relation between VDR gene polymorphism and the occurrence of HCC in chronic hepatitis C patients. This study included 102 subjects classified into three groups. Group A included 34 healthy subjects as control. Group B included 34 chronic hepatitis C patients with HCC. Group C included 34 chronic hepatitis C patients without HCC. Estimation of Apa-1 VDR gene polymorphism was performed by restriction fragment length polymorphism-Polymerase chain reaction (RFLP-PCR). Results In HCC group, C allele was more frequent than A allele (80.88% and 19.12%), respectively. In chronic hepatitis group, C allele was more frequent than A allele (64.71% and 35.29%), respectively. In control group, A allele was more frequent than C allele (73.53% and 26.47%), respectively. Genotype CC + CA was dominant in HCC group (91.18%) and chronic hepatitis group (79.41%). In the control group, the dominant genotype was AA (58.82%). Moreover, there was a significant relation between Apa-1 VDR genotype CC and tumor size. Conclusions There is an association between VDR Apa-1 polymorphism and the occurrence of HCC in chronic hepatitis C patients.

This work was performed to quantitatively determine doxycycline residues in different tissues of rabbits (kidneys, spleen, liver, muscles, heart and lungs) and sera following multiple oral doses of the drug using High Performance Liquid Chromatography. Moreover, the study aimed to estimate the withdrawal time of this drug in rabbit tissues. Twenty five healthy male New Zealand rabbits ranging from 2-2.5 kg body weight were used. Twenty one rabbits were given doxycycline directly into the stomach at a dosage of 10 mg/kg BW once daily for five successive days. Samples were analyzed at 1 st , 3 rd , 5 th , 7 th , 9 th , 15 th and 21 st day after last oral dose. The results indicated a widespread distribution of doxycycline in the samples, which remained within the detectable limit till the 3 rd day in most tested tissues. While in kidneys, spleen and liver, doxycycline remained till the 5 th day following the last oral administration of the drug. Therefore, it is recommended that rabbits treated with doxycycline must be slaughtered after the fifth day of drug administration to be safe for human consumption.

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