Storage of skin grafts for later use is one of the standard applications in surgery. It is the most preferred method to maintain at +4 C in refrigeration after wrapping the surplus grafts into sterile gauze pad moistened with saline. Although there are many studies on the storage of skin grafts, less is known about storing skin grafts with PRP.Twenty-five pieces of 1 × 1 cm 2 partial thickness skin graft were harvested from 12 patients during the reduction mammoplasty operation. Twenty-four grafts were divided into 4 groups, and each group consisted of 6 grafts, 1 graft was analyzed as Day 0. Grafts in Group 1, 2, and 3 were wrapped by sterile gauze pad moistened by either saline (Group 1) or Hartman (Group 2) or PRP (Group 3). Grafts were analyzed macroscopically and microscopically. There were no significant differences between media for the first 10 days. Decrease in viability was less in saline and PRP wrapped grafts at 20 day, viability decreased significantly in all environments after 20 days.Although there was no significant difference in saline or PRP storage, it was observed macroscopically that the grafts stored in the PRP appeared better.
K E Y W O R D Sgraft preservation, platelet rich plasma, skin graft storage
A B S T R A C TAim: To achieve homogenization, one of the most important factors to overcome during platelet-rich plasma (PRP) is standardization phases. Method: We used 10 cc 'BD vacutainer ACD-A' tubes which consisted of 1.5 cc ACD-A. 3 tubes blood was collected from healthy volunteers from 20 to 40 years. All tubes were centrifuged by 180g x 12 min. Then 0.01 ml PRP was collected from just the level of buffy coat. Results: Platelet concentration decreased from buffy coat to top of PPP as expected. If any counting done before homogenization the results will be misleading. Conclusion: When we sample 3 cc PRP homogenized with shaker at 3 point from 3 different point, we see that they are close to each other. It is important that the PRP to be used in the studies must be homogenized before any measurement.
Withdraw blood from rats is an important, but not easy, invasive procedure in experimental research on these animals. In addition, the preparation and standardization of platelet-rich plasma (PRP) is a more difficult process in rats. In this study, we presented our experiences about rat blood collection and PRP preparation technique. Methods and Result: This experimental study was performed with ten male Wistar rats weighing 250-300 g. Under anesthesia, the blood was obtained by percutaneous puncture from the right ventricle of the rats. The blood obtained from rats was rapidly transferred to tubes containing anticoagulants such as sodium citrate or acid citrate dextrose solution A. After the first centrifuge, the all plasma was collected by a pipette after a second spin in a sterile tube. As a result of all these processes, PRP at the desired concentration was obtained. Conclusions: Blood withdraw from rats is not an easy method, and when large amounts of blood are required cardiac blood intake is necessary. In order to achieve the therapeutic intensity in PRP preparation, usually a double spin is required and the concentration obtained with the base number of platelets should be compared.
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