Skin graft storage in platelet rich plasma (PRP)

Abstract

Storage of skin grafts for later use is one of the standard applications in surgery. It is the most preferred method to maintain at +4 C in refrigeration after wrapping the surplus grafts into sterile gauze pad moistened with saline. Although there are many studies on the storage of skin grafts, less is known about storing skin grafts with PRP.Twenty-five pieces of 1 × 1 cm 2 partial thickness skin graft were harvested from 12 patients during the reduction mammoplasty operation. Twenty-four grafts were divided into 4 groups, and each group consisted of 6 grafts, 1 graft was analyzed as Day 0. Grafts in Group 1, 2, and 3 were wrapped by sterile gauze pad moistened by either saline (Group 1) or Hartman (Group 2) or PRP (Group 3). Grafts were analyzed macroscopically and microscopically. There were no significant differences between media for the first 10 days. Decrease in viability was less in saline and PRP wrapped grafts at 20 day, viability decreased significantly in all environments after 20 days.Although there was no significant difference in saline or PRP storage, it was observed macroscopically that the grafts stored in the PRP appeared better. K E Y W O R D Sgraft preservation, platelet rich plasma, skin graft storage

Evaluation of skin natural change function in split-thickness skin graft

“…The aesthetic unit of the face must be considered during excision, which can often be performed using a hydrosurgery system such as Versajet (Smith & Nephew, Hull, UK) for shallow partial thickness burns (see image below). When excising burns on the dorsum of the hand, special care must be taken to avoid injury to the extensor tendons and peritenon in order to provide a vascularized bed for the corrupted to recover and maintain maximal postoperative hand function (Fukuoka et al, 2021;Gokkaya et al, 2020).…”

Use of autologous platelet-rich plasma for skin graft preservation: an experimental comparative study

“…The practical impact of the available data is the current recommendation that skin grafts be stored in saline for a maximum of 10 days before use. Grafts stored in this way for 10 days do not show changes affecting the overall viability of the graft [3,4]. ment of extensive burns.…”

“…This protocol may explain why their samples in saline were affected by maceration, since PRP has a higher viscosity [1]. In another study, Gokkaya et al reported that the samples stored in PRP had better final appearance, when examined macroscopically, although the final storage time did not significantly differ between samples stored in PRP versus saline [3].…”

“…This method is economical, as saline solution is inexpensive, as well as simple and not technically challenging. A limitation of this method is that skin grafts stored in saline at 4°C should optimally be used within 10 days of collection [3,4].…”

“…The literature describes other various methods of preserving skin grafts, including the possibility of their storage in platelet-rich plasma (PRP) [1][2][3]. PRP contains over 30 biogenic factors [1,5] that affect cell proliferation and differentiation, facilitate tissue remodeling, and accelerate or improve wound healing.…”