Indonesian black rice (Oryza sativa L., Ketan Hitam-2) is pigmented rice with high potency as a nutraceutical compound, especially with its high protein content. The effect of the germination period on antioxidant activities and angiotensin I-converting enzyme (ACE-I) inhibitory of Indonesian black rice seed protein was studied to determine its potential use as a nutraceutical ingredient. In this study, the bioactive peptide was produced by protein modification through the germination process for 0, 2, 4, and 6 days. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and amino acid composition were conducted to determine the changes in protein during the germination period. Two antioxidant methods (ABTS•+ and OH• ) were performed to determine the antioxidant activity and the defence against radical-mediated DNA damage by hydroxyl. Meanwhile, the antihypertensive potency was analysed by ACE-I inhibitor activity. The results showed that the antioxidant-protected hydroxyl radical-induced oxidative DNA damage and ACE-I inhibitor activities were increased during the germination period. The IC50 value of ABTS•+ and hydroxyl radical scavenging on the sixth-day germination were 28.18 μg/mL, and 24.84 μg/mL, respectively, lower than the control (before germinated). Moreover, the IC50 value of ACE-I Inhibitory activities during the six-day germination was (9.07 μg/mL). The above results indicated that the germination period could increase the activity of bioactive peptides in Indonesian black rice. It might be used in future nutraceuticals and human health applications.
Rice is a world-famous cereal food divided into pigmented and non-pigmented rice. Pigmented rice is popular as healthier food than non-pigmented rice due to its potency as an antioxidant. Nevertheless, the potential of pigmented rice has not been widely studied. Indonesian selected pigmented rice protein’s antioxidant potential and the non-protein compound were in-vitro studied. The antioxidant potencies were evaluated by extracting fresh seeds of nine pigmented rice (Aek Sibundong, Beureum Taleus, Gogo Niti-2, Lamongan-1, Merah SP, Merah Wangi, Mota, Ketan Hitam-2, and Super Manggis) and non-pigmented rice (IR-64) as control. Various free radical scavenging methods to determine the antioxidant activity (ABTS•+, DPPH•, OH• and O2-) were conducted. Meanwhile, the genetic classification was performed by a simple sequence repeat (SSR) marker to determine the relationship between varieties. The results showed that protein of Ketan Hitam-2 had the highest ABTS•+ radical scavenging (98.06%), followed by Beureum Taleus (42.54%). Ketan Hitam-2 protein also showed the highest OH• and O2- activities (43.49% and 6.02%, respectively). The highest DPPH• potency of the non-protein compounds also shown by Ketan Hitam-2 (32.23%) with the activity of OH• and O2- (20.63% and 14.56%, respectively). These results showed that Ketan Hitam-2 has the highest potency as an antioxidant, which could be recommended as a nutraceuticals compound.
The potential source of bioactive protein from pigmented rice seed was investigated by buffered extracting from nine varieties (Merah-SP, Gogo Niti-2, Merah Wangi, Super Manggis, Lamongan-1, Mota, Ketan Hitam-2, Beureum Taleus, and Aek Sibundong) and non-pigmented rice (IR-64) as a control. The potent contributor to free radical scavenging of extracted proteins was evaluated by analyzing their free amino acid composition. The free radical scavenging and angiotensin-I converting enzyme (ACE-I) inhibitory activity of these proteins were analyzed in-vitro. The free radical scavenging activity was analyzed using various standard methods, including radical cation 2,2-azinobis-3-ethylbenzothiazoline-6-sulphonate acid (ABTS •+ ), hydroxyl radical (OH • ) and defense against radical-mediated DNA damages by hydroxyl. Total free amino acid content ranged between 77.7-181.2 g/100 g protein, and among them, Ketan Hitam-2 displayed the highest abundant antioxidant amino acid grouping (23.23%) and shown significantly higher ABTS •+ activity (IC 50 =8.64 µg/mL) and OH • activity (IC 50 =20.33 µg/mL). Moreover, Ketam Hitam-2 also exhibited notable ACE-I inhibitory activity (IC 50 =6.20 µg/mL) and protected hydroxyl-induced oxidative damage to DNA. The in-vitro systems for free radical scavenging and ACE-I inhibitory were used to acquire the data. The potency of Ketan Hitam-2 seed protein could be utilized as a natural nutraceuticals compound.
This study aims to analyze the impact of supply chain management (SCM), total quality management (TQM), and just-in-time (JIT) practices on the quality performance of manufacturing companies in Jawa Barat, Indonesia. The manufacturing sector in Jawa Barat plays a crucial role in the regional economy, making it imperative for companies to maintain high-quality standards to remain competitive. Data were collected through surveys and interviews from a sample of 40 manufacturing companies in Jawa Barat. The analysis included statistical techniques such as t-tests and F-tests to examine the relationships between SCM, TQM, JIT, and quality performance. The results indicated that SCM, TQM, and JIT practices have a significant positive influence on quality performance. The calculated t-values and F-value were found to be greater than the critical values, indicating statistical significance. Furthermore, the Adjusted R Square value of 0.669 suggests that 66.9% of the variance in quality performance can be attributed to the combined influence of SCM, TQM, and JIT. These findings underscore the importance of implementing effective SCM, TQM, and JIT practices to enhance quality performance in manufacturing companies. By optimizing supply chain processes, fostering a culture of continuous improvement, and implementing lean production methods, companies can achieve higher product quality, customer satisfaction, and operational efficiency.
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