Plasma clearance, volumes of distribution, and renal and extrarenal clearances of digoxin were calculated from plasma digoxin concentrations and urinary excretion of digoxin after intravenous injection of digoxin in 8 subjects. The investigation was repeated in the same subjects during long-term treatment with spironolactone. Increased plasma concentration of digoxin was detected during spironolactone treatment. Calculated plasma and renal clearances of digoxin and the volumes of distribution decreased statistically significant. Near maximal capacity for the tubular secretion of digoxin was found when normal digoxin dosage was used. It is suggested that unless spironolactone decreases the myocardial sensitivity for digoxin, the loading dose as well as the maintenance dose of digoxin should be reduced during treatment with spironolactone.
The effect of reserpine, 5-hydroxytryptamine (5-HT), polymyxin B and fasting on the mast cells and tensile strength of healing wounds has been investigated.
Reserpine and polymyxin B were given during the last three days before the wounds were inflicted, while 5-HT was given as daily injections after the incisions. Reserpine and – to a lesser extent – 5-HT caused a significant decrease in the tensile strength of the wounds. This decrease was probably not caused by the concomitant weight-loss since a group of fasted animals which showed the same weight-loss turned out to have a normal tensile strength. The animals treated with polymyxin B showed no significant decrease in tensile strength. Treatment with reserpine and 5-HT was not followed by any significant changes in the mast cell morphology, while polymyxin B caused marked changes, the nature of which could not be determined.
It is concluded that the decrease in tensile strength of healing wounds induced by 5-HT and reserpine is probably not mediated by the tissue mast cell.
The incorporation of 35S sulphate and the total content of hexosamine in skin wounds was determined in thyroidectomized, normal, and thyroxine-treated guinea pigs. Seven days after the production of the wounds, all three groups showed an increase in the 35S incorporation and in the hexosamine content as compared with normal skin. While the relative incorporation of 35S sulphate was most intense among thyroidectomized guinea pigs, the three groups showed no difference in the content of hexosamine. Thyroidectomy induced a more rapid synthesis of sulphated mucopolysaccharide than that observed in the thyroxine-treated and control guinea pigs, whereas the total content of acid mucopolysaccharides in the wound tissues was not affected by either thyroidectomy or thyroxine.
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