A better knowledge of plant-microbial interactions will provide a basis for improving the efficacy of biological remediations. Further research is, however, needed to investigate different feedback mechanisms that select and regulate microbial activity in the rhizosphere.

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The potential environmental toxicity of zero-valent iron nanoparticles (nZVI) and three types of nanosilver differing in average particle size from 1 to 20 nm was evaluated using seed germination tests with ryegrass, barley, and flax exposed to 0-5000 mg L(-1) nZVI or 0-100 mg L(-1) Ag. For nZVI, germination tests were conducted both in water and in two contrasting soils to test the impact of assumed differences in bioavailability of nanoparticles. Inhibitory effects were observed in aqueous suspensions at 250 mg L(-1) for nZVI and 10 mg L(-1) for Ag. Reduction in shoot growth was a more sensitive endpoint than germination percentage. Complete inhibition of germination was observed at 1000-2000 mg L(-1) for nZVI. For Ag, complete inhibition was not achieved. The presence of soil had a modest influence on toxicity, and inhibitory effects were observed at 300 mg nZVI L(-1) water in soil (equivalent to 1000 mg nZVI kg(-1) soil). Complete inhibition was observed at 750 and 1500 mg L(-1) in sandy soil for flax and ryegrass, respectively, while for barley 13% germination still occurred at 1500 mg L(-1) . In clay soil, inhibition was less pronounced. Our results indicate that nZVI at low concentrations can be used without detrimental effects on plants and thus be suitable for combined remediation where plants are involved. Silver nanoparticles inhibited seed germination at lower concentrations, but showed no clear size-dependent effects, and never completely impeded germination. Thus, seed germination tests seem less suited for estimation of environmental impact of

Phytoremediation of organic pollutants depends on plant-microbe interactions in the rhizosphere, but the extent and intensity of such rhizosphere effects are likely to decrease with increasing distance from the root surface. We conducted a time-course pot experiment to measure dissipation of polycyclic aromatic hydrocarbons (PAHs) in the rhizosphere of clover and ryegrass grown together on two industrially polluted soils (containing 0.4 and 2 g kg(-1) of 12 PAHs). The impact of the fungal root symbiosis arbuscular mycorrhiza (AM) on PAH degradation was also assessed, as these fungi have previously improved plant establishment on PAH-polluted soils and enhanced PAH degradation in spiked soil. The two soils behaved differently with respect to the time-course of PAH dissipation. The less polluted and more highly organic soil showed low initial PAH dissipation rates, with small positive effects of plants after 13 weeks. At the final harvest (26 weeks), the amounts of PAHs extracted from nonplanted pots were higher than the initial concentrations. In parallel planted pots, PAH concentrations decreased as a function of proximity to roots. The most polluted soil showed higher initial PAH dissipation (25% during 13 weeks), but at the final harvest PAH concentrations had increased to values between the initial concentration and those at 13 weeks. An effect of root proximity was observed for the last harvest only. The presence of mycorrhiza generally enhanced plant growth and favored growth of clover at the expense of ryegrass. Mycorrhiza enhanced PAH dissipation when plant effects were observed.

Phytoremediation of soils polluted with polycyclic aromatic hydrocarbons (PAHs) has so far neglected the possible role of the ubiquitous symbiotic associations between plant roots and fungi known as arbuscular mycorrhizas. A time course laboratory experiment with clover and ryegrass grown on spiked [500 + 500 + 50 mg kg-1 of anthracene, chrysene and dibenz(a,h)anthracene] soil demonstrated for the first time that dissipation of condensed PAHs may be enhanced in the presence of arbuscular mycorrhiza [66 and 42% reductions in chrysene and dibenz(a,h)anthracene, respectively, versus 56 and 20% reductions in nonmycorrhizal controls]. Addition of a surfactant accelerated initial PAH dissipation but did not attain final PAH concentrations below those obtained with nonmycorrhizal plants. Toxicity tests (earthworm survival and bioluminescence inhibition in Vibrio fischeri) indicated that mycorrhiza reduced the toxicity of PAHs and/or their metabolites and counteracted a temporally enhanced toxicity mediated by surfactant addition. Phospholipid fatty acid profiles demonstrated that the imposed treatments altered the microbial community structure and indicated that the mycorrhiza-associated microflora was responsible for the observed reductions in PAH concentrations in the presence of mycorrhiza.