Weight-adjusted neonatal 17OH-progesterone cutoff levels improve the efficiency of newborn screening for congenital adrenal hyperplasia ABSTRACTObjective: To evaluate weight-adjusted strategy for levels of neonatal-17OHP in order to improve newborn screening (NBS) efficiency. Subjects and methods: Blood samples collected between 2-7 days of age from 67,640 newborns were evaluated. When N17OHP levels were ≥ 20 ng/mL, and a second sample was requested. We retrospectively analyzed neonatal-17OHP levels measured by Auto DELFIA-B024-112 assay, grouped according to birth-weight: G1: < 1,500 g, G2: 1,501-2,000 g, G3: 2,000-2,500 g and G4: > 2,500 g. 17OHP cutoff values were determined for each group using the 97. Keywords 21-hydroxylase deficiency; congenital adrenal hyperplasia; newborn screening; neonatal 17OH-progesterone levels; preterm and full-term newborns RESUMO Objetivo: Avaliamos retrospectivamente os valores da 17OHP ajustados para o peso ao nascimento para melhorar a eficiência da triagem neonatal. Sujeitos e métodos: 67.640 recém--nascidos com amostras coletadas entre 2-7 dias de vida. Uma segunda amostra foi solicitada na presença de testes com valores da 17OHP ≥ 20 ng/mL. 17OHP dosada pelo método DELFIA-B024-112 e correlacionada com o peso ao nascimento: G1 < 1.500 g, G2 1.501-2.000 g, G3 2.000-2.500 g e G4 > 2.500 g.

Cattle (Bos taurus and Bos indicus) are organised on the basis of leadership and dominance in such a manner that a disturbance by an external stressor causes negative effects on their health, productivity, well-being, and behaviour. One of these effects is the excessive release of glucocorticoids, which results in increased alertness. We evaluated the action of exogenous oxytocin (OT) on serum cortisol levels in crossbred Red Angus heifers. Twelve Red Angus crossbred heifers were moved daily from the pasture to the corral in weeks 1 and 2 for adaptation to human contact and handling in the cattle crush. In weeks 3 and 4, they were divided into two groups of six (T1 and T2). The T1 group was administered 20 IU (2 mL) of OT via intramuscular injection and the T2 group was administered 2 mL of saline solution 0.85% (SS). In weeks 5 and 6, they were only contained in the cattle crush for evaluation. On days 01, 07, 14, 21, 28, 35, and 42, blood samples were collected by jugular venepuncture in vacuum tubes without anticoagulants. Then, serum cortisol levels were measured using a radioimmunoassay. In the period of adaptation, during weeks 1 and 2, serum cortisol levels decreased in both the groups, with higher levels in the SS group; the same result was obtained in weeks 5 and 6. During treatment, however, there was a significant difference between the two groups in week 4, with a reduction in cortisol levels in the OT group. This result suggests a modulator effect of OT on neuroendocrine response to stress.

The purpose of this experiment was to evaluate the performance responses of castrated goats, submitted to two surgical wound treatments: conventional treatment with ointment made of zinc oxide, pine oil, kaolin and xylene and permethrin, phenoxybenzyl and DDVP spray (dichlorvos) and alternative phytotherapic treatment using herbal medicine with ointment made of Calendula offcinalis extract and associations. Twelve undefined breed goats with an average initial weight of 15.5 kg, raised in total confinement, were separated into two groups and arranged in a completely randomized design to constitute two treatments with six replicates each, in order to compare the performance of animals treated conventionally and with Calendula offcinalis extract and associations. We employed the orchiectomy (castration) technique by opening the scrotum and spermatic cord on day 0, and both groups were treated until complete healing of the surgical wound on the 51st day of trial. We analyzed the following biometric parameters: weight gain (kg), withers height gain (cm), croup height gain (cm), thotacic perimeter gain (cm) and body condition score. The results were analyzed using the Statistical Package SAEG and Tukey's test (P> .05). There was no significant difference between treatments (P> 0.05) for any of the parameters analyzed. All biometric characteristics, with the exception of body condition, increased during the evaluation period. There was no negative impact of the use of herbal medicine on the characteristics observed