The aim of this study was the introduction of a specific class of signals, the so-called perfect sequences (PSEQ), in a novel approach for sonotubometry of the Eustachian tube (ET). Sonotubometry using PSEQ stimuli was performed on 20 healthy subjects in order to gauge its potential for clinical applications. In a series of 320 measurements ET opening was probed, which was induced by dry and water swallowing, Toynbee maneuver, and yawning. All sonotubograms were analyzed with respect to their shape, increase of sound intensity, and opening duration. In 298/320 measurements (>93%) the subjects reported subjective ET openings. The evaluation of the recorded sonotubograms showed good detection of ET opening for the inducing maneuvers of swallowing (dry and water swallowing) and the Toynbee maneuver, with 90, 86, and 80% valid sonotubograms, respectively. Yawning led to only 40% valid sonotubograms. In total, 237/320 (~74%) sonotubograms were classified as valid. The evaluation of the sound level increase during ET openings showed that it was significantly higher in measurements with dry and water swallowing, as well as Toynbee maneuvers (mean 17.1, 19.0 and 17.2 dB, respectively), than with yawning (mean 10.17 dB; P < 0.0001). Nasal decongestion was found to have little influence on the results (P > 0.05). Sonotubometry using PSEQ stimuli is a novel sonotubometry methodology that provides valuable information regarding the auditory tube patency. By further technical refinements of the method, a diagnostic tool with high sensitivity and specificity could be developed.

PSEQ allowed the detection of an ET opening in all 40 normal ears (91% of the performed maneuvers) and in 87.5% of the ears with impaired ET function (but only 47% of the maneuvers). The average amplitude of the openings in healthy ears was 16.86 dB, the average duration was 363 ms. In diseased ears these values were significantly lower: 9.73 dB, p < 0.001 and 280 ms, p < 0.05.

Sonotubometry allows an assessment of the Eustachian tube (ET) function under physiological conditions. The reliability of the application of an 8 kHz pure-tone signal was investigated. In 40 normal subjects (80 ears) sonotubometric studies were performed with a custom-made device. ET opening was provoked by swallowing, yawning and Valsalva manoeuvre. An opening was detected in all patients but not in all manoeuvres. Four characteristic sonotubogram types were found. Most common was the spike-type (60%). The double-peak and the plateau-shaped curves occurred in 17% each. The finding of an descendant curve was rare (5%). Of 623 measurements, only in 55% manouvres a positive sonotubometric result was found despite the fact that the patients reported an opening in all cases. The median opening time in dry swallowing, liquid swallowing, yawning and Valsalva was found to be 486, 355, 1,263 and 1,250 ms. A median sound increase of 16.0, 13.8, 15.0 and 15.0 dB was recorded for these manoeuvres. There was a statistic significant difference (P < 0.02) between the increase in sound intensity of liquid and dry swallowing. There was also a statistic significant difference found for the duration of the forced manoeuvres Valsalva and yawning as compared to dry and liquid swallowing (P < 0.0001). The use of an 8 kHz pure-tone signal showed a limited sensitivity for the detection of ET openings. This is mainly due to noise pollution, but also because of an altered positioning and/or dislocation of the probes and compression of the nostrils. The application of an 8 kHz signal is therefore not reliable enough for the use in practice. Further technical refinements and the use of alternative signals are necessary for a broader clinical application.

The new approach is an alternative to pO2 histography and may play a future role in the planning of radiotherapy in the neck.

There are many diverse methods for the assessment of Eustachian tube (ET) function. Clinical studies have demonstrated good results for several clinical tests, tympanometry and a number of manometric approaches, such as the nine-step test. The relevance of tubomanometry according to Estève requires further investigation. MRI studies in particular have considerably enhanced our knowledge of ET function. Endoscopy and conventional Schüller radiography are routinely employed in the clinic. The pressure chamber is ideal for simulating the most diverse test conditions, but it is only used experimentally. Sonotubometry has both the potential and the necessary level of technical advancement for a broader clinical application. The majority of investigations have demonstrated that there is no single valid method. There is currently no technique available that is capable of reflecting all aspects of ET function. Although several methods have found clinical application, a commonly accepted golden standard for ET testing still has to be established. Further clinical studies are necessary to generate an adequate amount of data pertaining to the various ET function tests.