ABSTRAKRadikal bebas memiliki efek menyebabkan terjadinya kerusakan sel dalam tubuh sebagai pemicu berbagai penyakit. Antioksidan diketahui memiliki kemampuan untuk meredam radikal bebas. Telah dilakukan penelitian potensi aktivitas antioksidan dari ekstrak kental dan fraksi daun kopi robusta (Coffea robusta). Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 70 % selama 3 x 5 hari pada suhu ruangan, diperoleh ekstrak kental dengan rendemen 15,9 % b/b. Fraksinasi dilakukan dengan pelarut berdasarkan perbedaan kepolaran diperoleh fraksi n-heksan, fraksi etil asetat dan fraksi air. Uji antioksidan menggunakan alat Spektrofotometer Uv-Vis dengan metode penghambatan radikal bebas DPPH (2,2-Difenil-1-Pikrilhidrazil) terhadap ekstrak kental dan fraksi n-heksan, etil asetat dan air pada beberapa konsentrasi, yaitu 100 ppm, 80 ppm, 60 ppm, 40 ppm, 20 ppm. Hasil penelitian menunjukkan penghambatan radikal bebas (% Inhibisi) ekstrak dan fraksi meningkat sesuai peningkatan konsentrasi uji. Nilai % Inhibisi ekstrak kental, fraksi n-heksan, fraksi etil asetat dan fraksi air, pada konsentrasi 100 ppm berturut-turut adalah 93,7 %, 95,56 %, 69,54 % dan 61,28 %. Hasil perhitungan IC50 yang diperoleh untuk ekstrak kental adalah 43,83 ppm, fraksi n-heksan adalah 38,32 ppm, fraksi etil asetat adalah 37,07 ppm dan fraksi air adalah 73,62 ppm.Kata kunci: Antioksidan, daun kopi (Coffea robusta), DPPH (2,2-Difenil-1 Pikrilhidrazil), ekstraksi, fraksinasi ANTIOXIDANT OF EXTRACT AND FRACTION Coffea robusta LEAVES WITH DIPHENYLPICRYLHIDRAZYL (DPPH) METODH ABSTRACTFree radicals can damaged human body cells, antioxidant can inhibit the effect of free radical. The research was about potential antioxidant activity of extracts and fractions from Coffea robusta leaves. Extraction used maceration method with ethanol 70% for 3 x 5 days at room temperature, yield of extract was 15.9 % w/w. Fractionation based on differences in polarity, there were fraction of n-hexane, ethyl fractions and water fraction,. Antioxidant test used a UvVis Spectrophotometer with the method of inhibiting free radical DPPH (2,2-Diphenyl-1-picrylhidrazyl) to extract and fractions n-hexane, ethyl acetate and water at some concentration, 100 ppm, 80 ppm, 60 ppm, 40 ppm, and 20 ppm. The inhibition of free radicals (% ihibition) extract and fractions increased by a corresponding increase in concentration of the test. % inhibition value at 100 ppm for extract, fraction of n-hexane, fraction of ethyl acetate and the water fraction respectively were 93.7%, 95.56%, 69.54% and 61.28%,. IC50 of extract total was 43.83 ppm, fraction of n-hexane was 38.32 ppm, ethyl acetate was 37.07 ppm and water fraction was 73.62 ppm.
The synthesis of modified chitosan has been studied. The aims of this research were modification of chitosan with ZnO nanoparticle to form chitosan-ZnO nanoparticle and its application as antibacterial agent of Staphylococus aureus. Characterization of modified chitosan was conducted using FTIR spectroscopy and X-Ray diffractometer. ZnO nanoparticle was synthesized by leaf extract of Sirih hijau (piper betle L) and zinc acetate dihydrate. Modified chitosan was synthesized by chitosan and ZnO nanoparticle. Modified chitosan solution can act as antibacterial agent with paper disk method. The result showed that chitosan can be modified with ZnO nanoparticle and detected at wave number of 3427 cm−1. The crystalline size of ZnO nanoparticle is 16.47 nm. The average inhibition zone of chitosan-ZnO nanoparticle at concentration 10.000, 5.000 and 2.500 ppm are 28.87 ± 0.4 ; 24.93 ± 0.15 and 19.35 ± 0.3 mm respectively.
This Jengkol is one of the native plants in Indonesia. Jengkol beans are known as a popular food ingredient, and empirically the leaf is also used by people to treat hyperlipidemia. This study aimed to determine the antihyperlipidemic effect of Jengkol leaf extract (Achidendron jiringa) on male white rats induced by propylthiouracil and High fat diet. This was an experimental research with complete random design. Animals were divided into 5 groups: negative control group (tween 80), positive control (simvastatin 10 mg/kg BW), 3 other groups were given Jengkol leaf extract a dose of 100, 200, and 400 mg/kg BW. The result showed that there is a significant decline in total cholesterol levels after being given Jengkol leaf extract for 14 days at each dose respectively compared to the hyperlipidemic group. The percentages were 41.45%, 47.76%, 44.91%, and the simvastatin group was 56.70%. The study also revealed the optimal dose of Jengkol leaf extract that was 200 mg/kg BW
Background: Red betel leaf (P. crocatum Ruiz & Pav) possesses antibacterial properties, so the prospect is to be developed as a deodorant in lotion dosage forms. Objective: This study was conducted with the intention to prepare, evaluate, and investigate the stability of deodorant lotion preparations from the ethanolic extract of red betel leaf (P. crocatum Ruiz & Pav) based on stearic acid. Methods: The maceration method used 70% ethanol as solvent to obtain red betel leaf extract. Furthermore, the concentrated extract was formulated as a deodorant lotion formula into three formulas, FI, FII, and FIII, with varying concentrations of stearic acid 2, 4, and 6%, respectively. All the deodorant lotion formulas were evaluated, their characteristics and stability of organoleptic, pH, homogeneity, viscosity, spreadability, adhesion, and irritation properties. The physical stability test was done by cycling test. The storage stability test was evaluated for 28 days. Results: Evaluation of physical properties for 28 days showed that deodorant lotion was homogeneous and pH in the range of 5.5-6.4; viscosity, spreadability, and adhesion tests gave good results and stability. In the irritation test, all formulas showed no irritation. Statistical test paired T-test on stability testing during storage and cycling test showed that the best and most stable formula was F3 with 6% stearic acid concentration. Conclusion: Ethanolic extract of red betel leaf (P. crocatum Ruiz & Pav) can be formulated into a deodorant lotion preparation that is stable and good during storage. Keywords: deodorant lotion, Piper crocatum Ruiz & Pav, stearic acid
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