This study investigated the dynamics of cyanobacteria in two deep, eutrophic reservoirs in a semi-arid region of Brazil during periods of stratification and destratification. Four collections were carried out at each reservoir at two depths at three-month intervals. The following abiotic variables were analyzed: water temperature, dissolved oxygen, pH, turbidity, water transparency, total phosphorus, total dissolved phosphorus, orthophosphate and total nitrogen. Phytoplankton density was quantified for the determination of the biomass of cyanobacteria. The data were analyzed using CCA. Higher mean phytoplankton biomass values (29.8 mm 3 .L −1 ) occurred in the period of thermal stratification. A greater similarity in the phytoplankton communities also occurred in this period and was related to the development of cyanobacteria, mainly Cylindrospermopsis raciborskii (>3.9 mm 3 .L −1 ). During the period of thermal destratification, this species co-dominated the environment with Planktothrix agardhii, Geitlerinema amphibium, Microcystis aeruginosa and Merismopedia tenuissima, as well as with diatoms and phytoflagellates. Environmental instability and competition among algae hindered the establishment of blooms more during the mixture period than during the stratification period. Thermal changes in the water column caused by climatologic events altered other physiochemical conditions of the water, leading to changes in the composition and biomass of the cyanobacterial community in tropical reservoirs.

RESUMO -(Variação temporal da comunidade fitoplanctônica em curtos intervalos amostrais no reservatório de Mundaú, Nordeste brasileiro). O objetivo deste estudo foi detectar a influência dos fatores abióticos sobre a comunidade fitoplanctônica em um reservatório de abastecimento, em curtos intervalos amostrais. As amostras foram coletadas na subsuperfície (0,1 m) e fundo das regiões pelágica (8 m) e litorânea (2 m), durante dois períodos sazonais, estações seca e chuvosa. As variáveis abióticas analisadas foram: temperatura da água, oxigênio dissolvido, condutividade elétrica, sólidos totais dissolvidos, turbidez, pH, nitrogênio total, nitrito, nitrato, fósforo total, fósforo total dissolvido e ortofosfato. A biomassa fitoplanctônica foi determinada a partir de valores de biovolume. A influência das variáveis abióticas na dinâmica das espécies fitoplanctônicas foi determinada através da Análise de Correspondência Canônica. A biomassa algal variou de 1,17×10 4 a 9,21×10 4 µg.L -1 , sendo que as cianobactérias apresentaram valores de biomassa entre 1,07×10 4 e 8,21×10 4 µg.L -1 . A mais alta disponibilidade de fósforo, limitação de nitrogênio, pH alcalino e estabilidade térmica favoreceram florações de cianobactérias, especialmente durante o período seco. As diferenças entre os horários e as estações amostrais na distribuição da comunidade fitoplanctônica foram determinadas pelos valores de temperatura, pH, fósforo total e turbidez. As associações fitoplanctônicas que mais se destacaram foram do grupo funcional Sn, formado por Cylindrospermopsis raciborskii, que predominou no sistema em ambos períodos sazonais. Concluiu-se que o fitoplâncton apresentou uma forte influência das variáveis abióticas especialmente no estabelecimento de padrões sazonais de distribuição. Palavras-chave: Cylindrospermopsis raciborskii, eutrofização, nutrientes, reservatório de abastecimentoABSTRACT -(Temporal variation of the phytoplankton community at short sampling intervals in the Mundaú reservoir, Northeastern Brazil). The aim of this study was to determine how abiotic factors drive the phytoplankton community in a water supply reservoir within short sampling intervals. Samples were collected at the subsurface (0.1 m) and bottom of limnetic (8 m) and littoral (2 m) zones in both the dry and rainy seasons. The following abiotic variables were analyzed: water temperature, dissolved oxygen, electrical conductivity, total dissolved solids, turbidity, pH, total nitrogen, nitrite, nitrate, total phosphorus, total dissolved phosphorus and orthophosphate. Phytoplankton biomass was determined from biovolume values. The role abiotic variables play in the dynamics of phytoplankton species was determined by means of Canonical Correspondence Analysis. Algae biomass ranged from 1.17×10 4 to 9.21×10 4 µg.L -1 ; cyanobacteria had biomass values ranging from 1.07×10 4 to 8.21×10 4 µg.L -1 . High availability of phosphorous, nitrogen limitation, alkaline pH and thermal stability all favored cyanobacteria blooms, particularly during the dry season. Tem...

This study analysed vertical and temporal variations of cyanobacteria in a potable water supply in northeastern Brazil. Samples were collected from four reservoir depths in the four months; September and December 2007; and March and June 2008. The water samples for the determination of nutrients and cyanobacteria were collected using a horizontal van Dorn bottle. The samples were preserved in 4% formaldehyde for taxonomic analysis using an optical microscope, and water aliquots were preserved in acetic Lugol solution for determination of density using an inverted microscope. High water temperatures, alkaline pH, low transparency, high phosphorous content and limited nitrogen content were found throughout the study. Dissolved oxygen stratification occurred throughout the study period whereas temperature stratification occurred in all sampling months, with the exception of June. No significant vertical differences were recorded for turbidity or total and dissolved forms of nutrients. There were high levels of biomass arising from Planktothrix agardhii, Cylindrospermopsis raciborskii, Geitlerinema amphibium and Pseudanabaena catenata. The study demonstrates that, in a tropical eutrophic environment with high temperatures throughout the water column, perennial multi-species cyanobacterial blooms, formed by species capable of regulating their position in the water column (those that have gas vesicles for buoyancy), are dominant in the photic and aphotic strata.

a b s t r a c tThe aim of the present study was to evaluate the influence of seasonality on the behavior of phytoplankton associations in eutrophic reservoirs with different depths in northeastern Brazil. Five collections were carried out at each of the reservoirs at two depths (0.1 m and near the sediment) at three-month intervals in each season (dry and rainy). The phytoplankton samples were preserved in Lugol's solution and quantified under an inverted microscope for the determination of density values, which were subsequently converted to biomass values based on cellular biovolume and classified in phytoplankton associations. The following abiotic variables were analyzed: water temperature, dissolved oxygen, pH, turbidity, water transparency, total phosphorus, total dissolved phosphorus, orthophosphate and total nitrogen. The data were investigated using canonical correspondence analysis. The influence of seasonality on the dynamics of the phytoplankton community was lesser in the deeper reservoirs. Depth affected the behavior of the algal associations. Variation in light availability was a determinant of changes in the phytoplankton structure. Urosolenia and Anabaena associations were more abundant in shallow ecosystems with a larger eutrophic zone, whereas the Microcystis association was more related to deep ecosystems with adequate availability of nutrients. The distribution of Cyclotella, Geitlerinema, Planktothrix, Pseudanabaena and Cylindrospermopsis associations was different from that seen in subtropical regions and the substitution of these associations was related to a reduction in the eutrophic zone rather than the mixture zone.Published by Elsevier GmbH.

The aim of the present study was to test whether different degrees of human activity affect the diet of the Brazilian silverside Atherinella brasiliensis in two tropical estuaries. Fish were collected along the salinity gradient of two Brazilian estuaries, the heavily impacted Paraiba Estuary and the less impacted Mamanguape Estuary, in the dry and wet seasons. The findings confirm that A. brasiliensis has generalist feeding habits and is able to change its diet under different environmental conditions. The results indicate clear spatial (i.e. along the estuarine gradient) changes in diet composition in both estuaries, but diet was also influenced by the degree of anthropogenic disturbance. During the wet season in the nutrient enriched Paraiba Estuary, when human activity was higher, the diet of A. brasiliensis was poorer and dominated by few dietary items, reflecting the potential impoverishment of prey items in this heavily disturbed system. The specimens collected in the most affected estuary also had a greater frequency of micro-plastics and parasites in their stomachs, reflecting the greater degree of human disturbance in the estuary. The present findings suggest that the diet of A. brasiliensis could be a useful indicator of changes in the ecological quality of these and other tropical estuaries of the western Atlantic Ocean.

The aim of this work was to study the phytoplankton community composition at limnetic environment in

The aim of the present study was to investigate the zooplankton community at six reservoirs in the state of Pernambuco (Brazil). Zooplankton assemblages were identified using current literature and quantitatively analysed under a microscope and stereomicroscope. Concurrently to the sampling of zooplanktonic and phytoplanktonic communities, in situ measurements of abiotic variables, such as water temperature, dissolved oxygen and pH, were determined using field probes and transparency was determined with a Secchi disk. Total phosphorus concentrations were used for the determination of the Trophic State Index. The reservoirs were classified between eutrophic and hypertrophic, oxygenated, with pH varying from slightly acid to alkaline, high temperatures and low water transparency. A total of 27 zooplankton taxa were identified. Phytoplankton was represented by Cyanophyta, Chlorophyta, Baccilariophyta and phytoflagellates. The highest richness of species was observed for Rotifera (17), followed by Crustacea (8), Protozoa (1) and Insecta (1). Rotifers shared quantitative importance with Crustacea, which were mainly represented by juvenile forms. Jazigo Reservoir presented the highest diversity and equitability. Lowest diversity and equitability were recorded at the Poço da Cruz and Mundaú reservoirs, respectively. Dissimilarity was detected between the environments studied regarding zooplankton composition and structure.Keywords: zooplankton, reservoirs, diversity, Trophic Index State, northeast Brazil. Comunidade zooplanctônica de seis reservatórios no nordeste do BrasilResumo O objetivo deste trabalho foi estudar a estrutura e dinâmica do zooplâncton em seis reservatórios do Estado de Pernambuco. A comunidade zooplanctônica foi identificada com literatura atualizada e a densidade analisada sob microscópio e estereomicroscópio. Concomitantemente às coletas das comunidades zooplanctônicas e fitoplanctônicas, foram medidas in situ algumas variáveis abióticas como temperaturas da água e do ar, oxigênio dissolvido, condutividade elétrica, pH, turbidez, usando sondas de campo, transparência com disco de Secchi. As concentrações de fósforo total foram usadas para a determinação do Índice de Estado Trófico. Os reservatórios foram classificados entre eutrófi-cos e hipertróficos, oxigenados, pH levemente ácido a alcalino, temperaturas altas e baixos valores de transparência da água. O zooplâncton esteve composto por 27 táxons. O fitoplâncton foi representado pelas Cyanophyta, Chlorophyta, Baccilariophyta e Fitoflagelados. A maior riqueza de espécies foi observada para Rotifera, (17), seguida por Crustacea (8), Protozoa (1) e Insecta (1). Rotifera compartilhou a importância quantitativa com os Crustacea, estes representados principalmente pelas formas jovens. No reservatório de Jazigo, foram obtidos os maiores valores para diversidade e equitabilidade. Os valores mais baixos foram registrados em Poço da Cruz e Mundaú, respectivamente. Foi detectada dissimilaridade entre os ambientes estudados quanto à composição e est...

In recent decades, there have been frequent occurrences of the cyanobacterium Cylindrospermopsis raciborskii in northeastern Brazil. Little is known regarding the response of straight and coiled morphotypes to environmental conditions such as light intensity and water temperature. Samples were collected at the Mundaú reservoir (PE, Brazil) at six sampling depths in the dry and rainy season. Both morphotypes exhibited seasonal and vertical differences in densities. The reservoir was stratified in the dry season, with a predominance of the straight morphotype. The coiled morphotype exhibited greater densities in the lower strata and prove to be more susceptible to light. There was evident thermal de-stratification in the rainy season, with a predominance of the coiled morphotype in the surface layers. Thermal de-stratification favors an increase in both morphotypes by providing adequate conditions for growth, such as low light intensity and milder temperatures, which are characteristic of the winter season in the northeastern Brazil