Nanogels loaded with fluorescent dextran as a model drug are synthesized by the oxidation induced cross‐linking of water soluble redox responsive thiolated poly(amino acid) in miniemulsion without the introduction of any cross‐linker molecule. Two types of high energy methods, namely, ultrasonication and high pressure homogenization (HPH), are compared. Dynamic light scattering and transmission electron microscopy measurements confirm that spherical nanogels in 100–150 nm diameter range are prepared successfully by HPH method. Size and surface charge of the nanogels can easily be controlled by environmental pH. The release of encapsulated drug is triggered by the degradation of nanogels in reducing environment due to the cleavage of disulphide bonds.
Redox and pH responsive hydrogels were synthesized, which can serve as matrices for smart drug delivery systems exploiting the redox and pH gradients in the human body. Water soluble thiolated poly(aspartic acid), a biocompatible synthetic polymer, enables us to crosslink in water with a non-cleavable cross-linker, poly(ethylene glycol) diglycidyl ether. The permanent cross-linker establishes stable polymer hydrogels regardless of the redox environment, thus the gels swell but do not dissolve upon redox stimuli. The reversible response upon redox stimulus was induced by thiol-disulphide transformation inside the hydrogel. The degree of swelling and the stiffness of the macroscopic hydrogels were controlled by their chemical composition including thiol content and cross-linking ratio as well as the redox state of the hydrogels. The degree of swelling of the hydrogels showed strong pH dependence due to the polyelectrolyte nature of the polymer network. Release of a macromolecular model drug was considerably faster in reducing than in oxidising environment, which indicates the potential use of the synthesized hydrogels as redox responsive drug delivery systems. Nanogels were prepared in water-in-oil emulsion and displayed redox-responsive properties. The hydrodynamic diameter of the nanogels strongly increased upon the cleavage of the disulphide linkages in reducing solution without the disruption of the gels.
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