To review urinary protein biomarkers as potential non-invasive, easily obtainable, early diagnostic tools in renal cell carcinoma (RCC).
MethodsA PubMed database search was performed up to the year 2020 to identify primary studies reporting potential urinary protein biomarkers for RCC. Separate searches were conducted to identify studies describing appropriate methods of developing cancer screening programmes and detection of cancer biomarkers.
ResultsSeveral urinary protein biomarkers are under validation for RCC diagnostics, e.g. aquaporin-1, perilipin-2, carbonic anhydrase-9, Raf-kinase inhibitory protein, nuclear matrix protein-22, 14-3-3 Protein b/a and neutrophil gelatinaseassociated lipocalin. However, none has yet been validated or approved for clinical use due to low sensitivity or specificity, inconsistencies in appropriate study design, or lack of external validation.
ConclusionsEvaluation of biomarkers' feasibility, sample preparation and storage, biomarker validation, and the application of novel technologies may provide a solution that maximises the potential for a truly non-invasive biomarker in early RCC diagnostics.
In this article, we demonstrate the magneto-mechanic behavior in a fluid environment of perpendicularly magnetized microdiscs with antiferromagnetic interlayer coupling. When suspended in a fluid and under the influence of a simple uniaxial applied magnetic field sequence, the microdiscs mechanically rotate to access the magnetic saturation processes that are either that of the easy axis, hard axis, or in-between the two, in order to lower their energy. Further, these transitions enable the magnetic particles to form reconfigurable magnetic chains, and transduce torque from uniaxial applied fields. These microdiscs offer an attractive platform for the fabrication of fluid based micro- and nanodevices, and dynamically self assembled complex architectures.
Synthetic antiferromagnetic (SAF) particles with perpendicular anisotropy display a number of desirable characteristics for applications in biological and other fluid environments. We present an efficient and effective method for the patterning of ultrathin Ruderman-Kittel-Kasuya-Yoshida coupled, perpendicularly magnetised SAFs using a combination of nanosphere lithography and ion milling. A Ge sacrificial layer is utilised, which provides a clean and simple lift-off process, as well as maintaining the key magnetic properties that are beneficial to target applications. We demonstrate that the method is capable of producing a particularly high yield of well-defined, thin film based nanoparticles.
Synthetic antiferromagnetic particles with perpendicular magnetic anisotropy offer a highly desirable platform for use in fluidic applications. This work illustrates their high level of switching field tunability and demonstrates the ability to use particle design to overcome unfavourable hysteretic changes during patterning to manufacture functional, low switching field nanodiscs. This makes them ideal candidates for lab-on-chip technologies, such as microfluidic sorting or detection devices.
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