Essential oil of nutmeg (Myristica fragrans houtt) is one of the many potentials of nutmeg that has a high economic value although in North Sulawesi it has not been exploited to its full potential. This research was conducted to compare the yield and properties of Nutmeg oil extract from the seeds and mace of Talaud and North Minahasa-North Sulawesi. The oil extract was obtained by distillation and was further characterized in terms of color, solubility in ethanol, density, optical rotation, refractive index. Results of this research exhibited that oil of the mace of nutmeg from Talaud had a lighter appearance in color compared to that from North Minahasa, while the density of oil extracted from North Minahasa was more densed compared to Talaud in both seed and mace (0.923 and 0.938 respectively at 25°C). The reflective index of nutmeg oil from North Minahasa was slightly higher than of Talaud both from seed and mace (1.4834 and 1.493 at 25°C), while the optical rotations of oil extracted from the mace were between +6.90° to +9.80° and from the seed were +20.73° to +22.30°.
Tanaman dalugha (Cyrtosperma merkusii (Hassk.) Schott) merupakan tanaman endemik di Propinsi Sulawesi Utara yang perlu dilindungi dari serangan organisme pengganggu tumbuhan. Karakter morfologi dan Identifikasi hama sangat perlu untuk kajian ilmiah tentang budidaya tanaman endemik dalugha sebagai sumber pangan lokal di Sulawesi Utara. Penelitian ini bertujuan untuk mengetahui karakter morfologi dan identifikasi hama pada tanaman dalugha. Lokasi penelitian di desa Moronge, Kecamatan Moronge, Kabupaten Kepulauan Talaud. Penelitian ini menggunakan metode deskriptif kualitatif, pengambilan sampel hama ditentukan dengan teknik purposive sampling. Karakter morfologi dan identifikasi hama dilakukan dengan menggunakan buku kunci determinasi serangga hama dan aplikasi google lens dengan tingkat akurasi pengenalan spesies hewan yang tepat. Hasil penelitian menunjukkan bahwa adanya keragaman hama pada tanaman dalugha yang terbagi dalam tiga kelas: Kelas Gastropoda yaitu Bekicot (Achatina fulica), Keong semak (Bradybaena similaris), Siput hijau (Rhinocochlis nasuta), Siput pita (Caracolus marginella), Siput kebun (Cornu aspersum), dan Sumpil (Subulina octona); Kelas Insekta yaitu Belalang hijau (Oxya servile), Jangkrik semak (Tettigonia caudate), Kumbang daun (Asphaera lustrans), Kepik kaki daun tumbuhan (Leptoglossus australis), Kutu kebul (Bemisia tabaci), Semut kebun hitam (Lasius niger), Ulat tanduk talas (Theretra oldenlandiae), Kecoa (Periplaneta americana L.), dan Kecoa surinam (Pycnoscelus surinamensis); serta Kelas Oligochaeta yaitu Cacing tanah (Lumbricus rubellus).Kata kunci: dalugha; hama; identifikasi; morfologi; Sulawesi Utara Morphological Characteristics and Pest Identification on Dalugha Plants (Cyrtosperma merkusii (Hassk.) Schott) in Talaud Islands District, North Sulawesi Province ABSTRACTDalugha plants (Cyrtosperma merkusii (Hassk.) Schott) are endemic plants in North Sulawesi Province which need to be protected from attack by plant-disturbing organisms. Morphological characters and identification of pests are very necessary for scientific studies on the cultivation of endemic plants of dalugha as a local food source in North Sulawesi. This study aims to determine the morphological characters and identification of pests in dalugha plants. The research location is in Moronge Village, Moronge District, Talaud Islands Regency. This study used a qualitative descriptive method, the sampling of pests was determined by purposive sampling technique. Morphological characters and identification of pests were carried out using the key book for insect pest determination and the Google Lens application with an accurate level of recognition of animal species. The results showed that there was a diversity of pests in Dalugha plants which were divided into three classes: Gastropod class namely Bekicot (Achatina fulica), Keong semak (Bradybaena similaris), Siput hijau (Rhinocochlis nasuta), Siput pita (Caracolus marginella), Siput kebun (Cornu aspersum), and Sumpil (Subulina octona); Class Insects are Belalang hijau (Oxya servile), Jangkrik semak (Tettigonia caudate), Kumbang daun (Asphaera lustrans), Kepik kaki daun tumbuhan (Leptoglossus australis), Kutu kebul (Bemisia tabaci), Semut kebun hitam (Lasius niger), Ulat tanduk (Theretra oldenlandiae), Kecoa (Periplaneta americana L.), and Kecoa surinam (Pycnoscelus surinamensis) and Oligochaeta class namely Cacing tanah (Lumbricus rubellus).Keywords: dalugha; identification; morphology; North Sulawesi; pest
North Sulawesi is located in the North-Eastern regions of the Indonesian Archipelago. As it is with most parts of Indonesia, North Sulawesi is dependent on rice as their staple food. Not all islands within the North Sulawesi cluster of islands can grow paddy or corn for their carbohydrate needs or starch ingredients in their diet and must have them shipped from other places with the dire consequences of transportation costs. The endemic biodiversity of these islands also has many plants with the potential as an alternative food carbohydrate source. This study was to explore these endemic plants for potential food ingredients for starch replacements for rice and corn. The many diversities available for these starch ingredients amongst them are tuber sources (Taro – Colocasia esculenta, Dalugha – Cyrtosperma merkusii, Gembili – Discorea esculenta and Banggai – Discorea alata) and perimedular sources (Sago Tanah and Sago Baruk). Many studies have researched these plants individually in various originating from many other parts of the world, but not many have made a head-to-head comparison and explored the potentials of these underutilized plants as starch food ingredients. This study extracted starches of these plants in a standardized manner and compared the properties side-by-side. These plants were found to be potentially healthy alternatives to rice as functional food starch with lower digestibility which would lead to lower GI staples, especially for the livelihoods of North Sulawesi coastal dwellers.
Abstract. Ginting EL, Wantania LL, Moko EM, Tumbol RA, Siby MS, Wulur S. 2021. Isolation and identification of thermophilic amylolytic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. Biodiversitas 22: 3326-3332. The aims of the research were to isolate and identify the amylase-producing thermophilic bacteria from Likupang Marine Hydrothermal, North Sulawesi, Indonesia. The bacteria were characterized based on the colony and cell morphology and subsequently screened for their amylase activities. The bacterial isolates were identified based on 16S rRNA gene sequences. There were 12 thermophilic bacteria isolates from Likupang Marine Hydrothermal that were able to produce amylase. Two selected isolates (L3 and L9) had an amylolytic index value in the range of 3.04-3.52 at 55oC. The colonies of L3 and L9 are circular, and they are Gram positive, rod-shaped, and motile bacteria. Based on the 16S rRNA gene sequences and phylogenetic tree analysis showed that L3 was closely related to Bacillus caldotenax with 93% similarity while L9 had 99% similarity to B. caldotenax. Hence, we identified L3 isolate as Bacillus sp. strain L3 and L9 isolate as B. caldotenax strain L9. Both of these bacteria were grouped as Geobacillus thermoleovorans.
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