ÖZAmaç: Mezenkimal Kök Hücreler (MKH) kendi kendini yenileme ve çeşitli hücre tiplerine farklılaşma yeteneğine sahiptir. Rejeneratif tıpta kullanımları günümüzde yaygın hale gelmiştir. Bununla birlikte, başarılı bir klinik uygulama için, MKH'lerin canlılığının ve potentliğinin transplantasyon öncesi hazırlık ve nakliye sırasında korunması gerekmektedir. Bu çalışmanın amacı; yeterince araştırılmamış olan bu konuda klinik olarak amaçlanan MKH etkinliğini korumak için önemli olan saklama koşulları ile ilgili literatüre katkı sağlamaktır. Gereç ve Yöntem: 4°C'de ve oda sıcaklığında (24°C), Phosphate Buffered Saline (PBS) ve Serum Fizyolojik(SF) solüsyonunda kısa süreli in vitro depolamada MKH'lerin canlılığı ve CD73, CD90, CD105, CD19 yüzey antijenleri değerlendirildi. Sonuç: 4°C ve 24°C'de PBS ve SF solüsyonunda canlılık oranları ve yüzey antijenleri açısından sonuçlar benzer olsa da, PBS'te ve 4°C'de daha yüksek oranlar elde edilmiştir. Farklı ortam şartlarında MKH'lerin farklılaşma, yaşlanma ve çoğalma kapasiteleri analiz edilerek ileri araştırmalar yapılabilir.
Objective: The aim of our study was to evaluate sonographic endometrial thickness succeeding the estrogen-only stage and on the
day when embryo transfer (ET) occurred in artificial frozen embryo transfer (FET) cycles to delve into the effect of endometrial
compaction (EC) on clinical pregnancy rate (CPR).
Patients and Methods: In the first group endometrial thickness diminished when ET occurred when compared to the end of the
estrogen-only phase (n:37). Endometrial thickness increased/did not alter for the second group (n:70).
Demographic characteristics were recorded and the following were studied: in vitro fertilization (IVF) treatment indications, hormone
levels, total antral follicle count, duration of infertility, embryo quality, embryo-fundus distance, endometrial thickness at the end of
estrogen-only phase and on ET day, luteal support, CPR.
Results: No significant difference occurred in CPRs (n:107). ET, on day 5 was higher in the first group (p
The relationship between oxidative stress and unexplained infertility (UEI) has not been studied in detail. This is the first study to evaluate dysfunctional high-density lipoprotein (HDL) by the myeloperoxidase (MPO) and paraoxonase (PON) ratio to investigate the role of oxidative stress in UEI. Materials and Methods: Patients with UEI (study group, n = 40) and male factor infertility (control group, n = 36) were included in this prospective study. Demographics and laboratory assessments were analyzed. Results: Total dosages of gonadotropin were higher in UEI when compared to the control group (p = 0.033). Number of Grade 1 embryos and the quality of blastocysts were lower in UEI than in the control group (p = 0.024, p = 0.020, respectively), whereas serum MPO/PON ratio was higher in UEI (p = 0.042). Stepwise linear regression analysis revealed that serum MPO/PON ratio levels could significantly predict the duration of infertility (p = 0.012). Conclusions: Serum MPO/PON ratio increased in patients with UEI, whereas the number of Grade 1 embryos and the quality of blastocysts decreased. Similar clinical pregnacy rates were found in both groups but the ET on day five is associated with higher clinical pregnancy rate in the male factor infertility.
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