Over the past decade, nitrogen (N) loads to Narragansett Bay have decreased by more than 50%. These reductions were, in large part, the direct result of multiple wastewater treatment facility upgrades to tertiary treatment, a process which employs N removal. Here we document ecosystem response to the N reductions and assess how the distribution of sewage N in Narragansett Bay has changed from before, during, and shortly after the upgrades. While others have observed clear responses when data were considered annually, our seasonal and regional comparisons of pre-and post-tertiary treatment dissolved inorganic nitrogen (DIN) concentrations and Secchi depth data, from bay-wide surveys conducted periodically from the early 1970s through 2016, resulted in only a few subtle differences. Thus we sought to use stable isotope data to assess how sewage N is incorporated into the ecology of the Bay and how its distribution may have changed after the upgrades. The nitrogen (δ 15 N) and carbon (δ 13 C) stable isotope measurements of particulate matter served as a proxy for phytoplankton, while macroalgae served as short-term integrators of water column bio-available N, and hard clams (Mercenaria mercenaria) as integrators of water column production. In contrast to other estuarine stable isotope studies that have observed an increased influence of isotopically lower marine N when sewage N is reduced, the opposite has occurred in Narragansett Bay. The tertiary treatment upgrades have increased the effluent δ 15 N values by at least 2‰. The plants and animals throughout Narragansett Bay have similarly increased by 1-2‰, on average. In contrast, the δ 13 C values measured in particulate matter and hard clams have declined by about the same amount. The δ 15 N results indicated that, even after the N-reductions, sewage N still plays an important role in supporting primary and secondary

Tropical urban estuaries are severely understudied. Little is known about the basic biogeochemical cycles and dominant ecosystem processes in these waterbodies, which are often low lying and heavily modified. The San Juan Bay Estuary (SJBE) in San Juan, Puerto Rico, is an example of such a system. Over the past 80 years, a portion of the estuary has filled in, changing the hydrodynamics and negatively affecting water quality. Here we sought to document these changes using ecological and biogeochemical measurements of surface sediments and bivalves. Measurements of sediment physical characteristics, organic matter content, and stable isotope ratios (δ13C, δ15N, δ34S) illustrated the effects of the closure of the Caño Martín Peña (CMP) on the hydrology and water quality of the enclosed and semi‐enclosed parts of the estuary. The nitrogen stable isotope (δ15N) values were lowest in the CMP, the stretch of the SJBE that is characterized by waters with low dissolved oxygen and high fecal coliform concentrations. Despite this, the results of this study indicate that nitrogen (N) contributions from N‐fixing, sulfate‐reducing microbes may meet or even exceed contributions from urban runoff and sewage. While the importance of sulfate reducers in contributing N to mangrove ecosystems is well documented, this is the first indication that such processes could be dominant in an intensely urban system. It also underscores just how little we know about tropical coastal ecosystems in densely populated areas throughout the globe.

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Salt marshes have the potential to intercept nitrogen that could otherwise impact coastal water quality. Salt marsh plants play a central role in nutrient interception by retaining N in above-and belowground tissues. We examine N uptake and allocation in two dominant salt marsh plants, short-form Spartina alterniflora and Distichlis spicata. Nitrogen uptake was measured using 15 N tracer experiments conducted over a four-week period, supplemented with stem-level growth rates, primary production, and microbial denitrification assays. By varying experiment duration, we identify the importance of a rarely-measured aspect of experimental design in 15 N tracer studies. Experiment duration had a greater impact on quantitative N uptake estimates than primary production or stem-level relative growth rates. Rapid initial scavenging of added 15 N caused apparent nitrogen uptake rates to decline by a factor of two as experiment duration increased from one week to one month, although each experiment shared the qualitative conclusion that Distichlis roots scavenged N approximately twice as rapidly as Spartina. We estimate total N uptake into above-and belowground tissues as 154 and 277 mg N•m −2 •d −1 for Spartina and Distichlis, respectively. Driving this pattern were higher N content in Distichlis leaves and belowground tissue and strong differences in primary production; Spartina and Distichlis produced 8.8 and 14.7 g biomass•m −2 •d −1. Denitrification potentials were similar in sediment associated with both species, but the strong species-specific difference in N uptake suggests that Distichlis-dominated marshes are likely to intercept more N from coastal waters than are short-form Spartina marshes.