The recent observation of Gibberella fujikuroi (Sawada) Wollenworth (teleomorph) (anamorf: Fusarium moniliforme Sheld.) Fusarium fujikuroi Nirenberg (anamorph), F. moniliforme J. Sheld. (synonym), the causal agent of Bakanae disease in rice fields, provides an opportunity to observe the characteristics of the pathogen and the possibility of prevention in Kocani area. Plant material with Gibberella fujikuroi symptoms was collected from rice (Oryza sativa) over the period of 3 years (from 2006 to 2008). Within this study, the presence and damage caused by this pathogenic fungus were confirmed. The objective of this study was a continuous field observation of symptoms in order to establish the percentage of infection and use of laboratory methods for proper protection
BACKGROUND:Fear of the dentist and dental treatment is a common problem. It can cause treatment difficulties for the practitioner, as well as severe consequences for the patient. As is known, the level of stress can be evaluated thought electrodermal activity, cortisol measure in saliva, or indirectly by psychometric tests.AIM:The present study examined the psychological influence of dental interventions on the child as well as coping patterns used for stress diminution.METHODS:We examined two matched groups of patients: a) children with orthodontic problems (anomalies in shape, position and function of dentomaxillofacial structures) (N = 31, mean age 10.3 ± 2.02) years; and b) children with ordinary dental problems (N = 31, mean age 10.3 ± 2.4 years). As psychometric instruments, we used: 45 items Sarason’s scale for anxiety, 20 items simple Stress - test adapted for children, as well as A - cope test for evaluation coping patterns.RESULTS:Obtained scores confirmed the presence of moderate anxiety in both groups as well as moderate stress level. For Sarason’s test obtained scores for the group with dental problems are 20.63 ± 8.37 (from max 45); and for Stress test 7.63 ± 3.45 (from max 20); for the orthodontic group obtained scores are 18.66 ± 6.85 for Sarason’s test, while for the Stress test were 7.76 ± 3.78. One way ANOVA confirmed a significant difference in values of obtained scores related to the age and gender. Calculated Student t - test shows non-significant differences in obtained test results for both groups of examinees. Coping mechanisms evaluated by A - cope test shows that in both groups the most important patterns used for stress relief are: developing self-reliance and optimism; avoiding problems and engaging in demanding activity.CONCLUSION:This study confirmed that moderate stress level and anxiety are present in both groups of patients (orthodontic and dental). Obtained scores are depending on gender and age. As more used coping patterns in both groups are developing self-reliance and optimism; avoiding problems and engaging in demanding activity. Some strategies for managing this problem are discussed.
SUMMARYThis study was carried out to determine the correlation between agronomic traits and seed yield of sunflower cultivars and also to determine the direct and indirect effect of analyzed traits on seed yield. The primary task in sunflower breeding programs is increasing the seed yield and creates new hybrids with high potential for seed and oil yield.Field trials were set up according to randomized block design during two growing seasons (2013 and 2014), on research area of Faculty of Agriculture, "Goce Delchev" University in Ovche Pole locality, Republic of Macedonia. As an experimental material were used 20 sunflower varieties. 1 000 seed weight, length and width of grain, oil content, oleic acid content and seed yield were analyzed. For 1 000 seed weight was obtained highly positive and significant correlation with seed yield and grain width. On the other hand, 1 000 seed weight showed negative and significant correlation with oil content. This trait expressed the biggest positive direct effect on seed yield.
Impact assessments of environmental pollutants are important in eco-genotoxicology. A random amplified polymorphic DNA (RAPD) technique was used to detect genotoxicity-induced DNA damage in Phaseolus vulgaris L. from heavy metals at two different concentrations. The results from six 10-base pair (bp) random RAPD primers with 60-70% GC content used, showed a total of 295 RAPD fragments of 700-4000 bp in molecular size in the seedlings of untreated and treated samples, of which only 163 fragments were polymorphic. Polymorphisms became evident as the disappearance and/or appearance of DNA fragments in treated samples compared to the control. A dendrogram constructed using the Numerical Taxonomy and Multivariate Analysis System (NTSYSps), showed that the control group merged with groups treated with CuSO(4)·5H(2)O (150 mg L(-1)) and MnSO(4)·H(2)O (150 mg L(-1)) in a separate cluster. These groups were linked with all of the other samples treated with metals at concentrations of 150 mg L(-1) and CuSO(4)·5H(2)O and Cd(NO(3))(2) at concentrations of 350 mg L(-1). Finally, the samples treated with metals at concentrations of 350 mg L(-1) together with NiSO(4) at the concentration of 150 mg L(-1), clustered separately. The DNA polymorphism detected by RAPD analysis offered a useful biomarker assay for the detection of toxic chemicals genotoxicity in plant model systems.
Grapevine (Vitis vinifera L.) is an economically important crop and can host several different viruses, including those that have to be excluded from certified propagating material in Europe. Among these, the Vitivirus Grapevine virus A (GVA) and the Maculavirus Grapevine fleck virus (GFkV) are phloem-limited viruses that are associated with two different grapevine diseases, Kober stem grooving, belonging to the rugose wood complex, and fleck diseases, respectively. During a survey conducted in 2012 in the former Republic of Macedonia, symptomatic plants with reddening of leaves were collected for laboratory analyses. In this study, grapevine red varieties (Vranec, Francovka, and Pinot noir) from four different localities (Stip, Kavadarci, Valandovo, and Gevgelija) in Macedonia were examined. Thirty-four samples were analyzed by DAS-ELISA using commercially antibodies against Grapevine leafroll associated virus-3 (GLRaV-3). Ten selected samples were processed through DAS-ELISA and molecular assays also for the presence of GVA and GFkV. Total RNA was extracted as previously described (2) and retro-transcribed (RT) using random primers followed by PCR assay with primers GVA-MP (5′-GCCAGAGGTGTTTGAGACAAT-3′) and GVA-CPdt (5′-TTTTGTCTTCGTGTGACAACCT-3′) (1), which amplified a GVA-specific fragment of 986 bp, and with primers GFkV-U279 (5′-TGGTCCTCGGCCCAGTGAAAAAGTA-3′) and GFkV-L630 (5′-GGCCAGGTTGTAGTCGGTGTTGTC-3′) (3), which amplified a GFkV-specific region of 315 bp. Results from DAS-ELISA test showed the presence of GLRaV-3 in 21 tested samples and of GVA and GFkV in six and three out of 10 selected samples, respectively. GVA was found in Vranec and Francovka vines sampled in all the locations mentioned before, while GFkV was detected in Vranec and Pinot noir vines, in Stip, Kavadarci, and Gevgelija. These latter results were confirmed by RT-PCR assays; then, four GVA-specific and three GFkV-specific amplicons were sequenced from both directions to get a 3× coverage. For GVA fragment, a primer pair designed in the internal part of the sequence was also used. BLASTn analyses showed that (i) PCR products amplified with GVA-specific primers shared best nucleotide sequence identities, ranging from 91.7 to 93.7%, with GVA isolate at GenBank Accession No. X75433; (ii) PCR products amplified with GFkV-specific primers shared best nucleotide sequence identities, from 92.5 to 94.7%, with GFkV isolate at AJ309022. These evidences reinforced the serological and PCR results indicating that GVA and GFkV were identified in examined grapevine plants in this study. Nucleotide sequences of GVA (KF594432 to 35) and GFkV (KF594429 to 31) were submitted to GenBank. To our knowledge, this is the first report of GVA and GFkV grapevine viruses in the Former Yugoslav Republic of Macedonia. References: (1) J. De Meyer et al. Page 138 in: Extended Abstracts, 13th Meeting of ICVG, Adelaide, 12-17 March 2000. (2) D. J. MacKenzie et al. Plant Dis. 81:222, 1997. (3) B. J. Shi et al. Ann. Appl. Biol. 142:349, 2003.
Evaluation and characterization of sunflower hybrids using morphological, physiological and biochemical data are necessary and essential in sunflower breeding programs. The aim of this paper was to evaluate the productive possibilities of some sunflower hybrids grown in Macedonian environmental conditions. The experiment was carried out during the period 2013 and 2014 on the research field of the Faculty of Agriculture, “Goce Delchev” University – Shtip, in Ovche Pole locality, Republic of Macedonia. Total 20 sunflower hybrids were used as an experimental material. The trial was arranged as randomized complete block design (RCBD) with three replications. The average content of oil and oleic acid were 45.6 and 65.2%, respectively. The highest seed yield from all sunflower hybrids was obtained for the hybrid NLK12M144 (3,344 kg ha–1) and the lowest for hybrid NLK12S126 (2,244 kg ha–1). Cluster analysis classified the sunflower hybrids into four groups based on agronomic traits and seed yield. Most of the hybrids were included in cluster I and III (7 hybrids) followed by cluster IV. Using principal component analysis two main components have been extracted, with eigenvalue greater than one. They account for 72.99% of the variability in the original data. Only four hybrids had positive values for both main components (NLK12M144, NLK12S070, NLK12S125 and NLN12N011 DMR). The results from this study can be used to identify the best sunflower hybrids grown under Macedonian environmental conditions and to select the superior hybrids for future exploitation.
Diseased barley plants (Hordeum vulgare), were noticed in the area of Kumanovo, Bitola, Probistip, Skopje and Kocani, at the beginning of March, 2006. Our investigations were carried out in the period from 2006 to 2009. The plants were highly diseased, probably in the stage of germination, dwarfed with necrotic leaves and with poorly developed root. A rotten root collar was noticed notice in some plants, which could be easily pulled out from the soil. Plants infected in a later developing stage became yellow from the top of the leaf, and many brown-olive, oval shape lesions were noticed. Conidia of Bipolaris sorokiniana (Sacc.) Shoen., were isolated from symptomatic lesions. Pseudothecia with asci and ascospores from teleomorph Cochliobolus sativus, were found on the barley straw in the same field the previous year
Primary purpose of restorative dentistry is to preserve pulp vitality. Besides calcium hydroxide, the application of calcium silicate cements as a material for direct pulp capping has become used recently. The aim of our study was to investigate the influence of materials for direct and indirect pulp capping on the bond strength of composite restorations using two different self etch dentin adhesives. The test was performed on 60 intact molars extracted for periodontal or orthodontic reasons. The prepared samples were divided into the two groups and three subgroups depending on the adhesive and pulp cupping material. Two different calcium silicate based materials were used for pulp cupping. One step and two steps self etch dentin adhesive was applied to prepared specimens depending on the group and with the help of a specially made metal mold set up a composite post. The share bond strength was assessed using a universal testing machine. The results showed significant difference in the share bond strength between the samples treated with TheraCal LC and Biodentine with TheraCal LC being superior to Biodentine.
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