Assessment of the genotoxicity of heavy metals in<i>Phaseolus vulgaris L</i>. as a model plant system by Random Amplified Polymorphic DNA (RAPD) analysis

Ackova, Darinka Gjorgieva; Kadifkova-Panovska, Tatjana; Mitrev, Saša; Kovacevik, Biljana; Kostadinovska, Emilija; Baceva, Katerina; Stafilov, Trajče

doi:10.1080/10934529.2012.645784

Cited by 12 publications

(6 citation statements)

References 32 publications

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“…Cd toxicity also affects the plasma membrane of plants, which could be attributed to electrolyte leakage [58], and membrane proteins like H-ATPase inhibition [42]. It has been known to affect the DNA repair system [59]; thus, the stability of the genomic template was distinctly reduced in Phaseolus vulgaris [60] and peas [61] in response to the direct application of Cd.…”

Section: Plant Responses To Cadmium Toxicitymentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

158

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Cadmium (Cd) is one of the most toxic metals in the environment, and has noxious effects on plant growth and production. Cd-accumulating plants showed reduced growth and productivity. Therefore, remediation of this non-essential and toxic pollutant is a prerequisite. Plant-based phytoremediation methodology is considered as one a secure, environmentally friendly, and cost-effective approach for toxic metal remediation. Phytoremediating plants transport and accumulate Cd inside their roots, shoots, leaves, and vacuoles. Phytoremediation of Cd-contaminated sites through hyperaccumulator plants proves a ground-breaking and profitable choice to combat the contaminants. Moreover, the efficiency of Cd phytoremediation and Cd bioavailability can be improved by using plant growth-promoting bacteria (PGPB). Emerging modern molecular technologies have augmented our insight into the metabolic processes involved in Cd tolerance in regular cultivated crops and hyperaccumulator plants. Plants’ development via genetic engineering tools, like enhanced metal uptake, metal transport, Cd accumulation, and the overall Cd tolerance, unlocks new directions for phytoremediation. In this review, we outline the physiological, biochemical, and molecular mechanisms involved in Cd phytoremediation. Further, a focus on the potential of omics and genetic engineering strategies has been documented for the efficient remediation of a Cd-contaminated environment.

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Section: Plant Responses To Cadmium Toxicitymentioning

confidence: 99%

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Raza

Habib

Najafi-Kakavand

et al. 2020

Biology

158

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“…У проростках пшениці Triticum aestivum L. зміну RAPD-спектрів відмічали при вирощуванні в присутності 5 мг/мл (~25 мкM) хлориду кадмію впродовж 9 діб [31]. Мутагенну дію кадмію на Phaseolus vulgaris L. спостерігали при культивуванні проростків у присутності Cd(NO 3 ) 2 в концентрації 150 мг/мл (0,61 мМ) впродовж 7 діб [32].…”

Section: обговоренняunclassified

Enhanced tolerance of Deschampsia antarctica Desv. to the mutagenic effect of cadmium ions

Spiridonova¹,

Андреев²,

Zahrychuk³

et al. 2016

Visn. ukr. tov. genet. sel.

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Aim. To study the potential effects of different concentrations of cadmium ions on antarctic plant D. antarctica using PCR analysis. Methods. Plants were grown in vitro on B5 Gamborg and Eveleigh agar medium supplemented with CdCl2. Genetic rearrangements were studied by PCR-analysis using ISSR- and IRAP-primers. Results. Genetically identical plants of D. antarctica obtained by microclonal propagation in vitro were used for the study of mutagenic effect of cadmium ions. The influence of Cd2+ was investigated within the concentration ranging from 0.1 to 10 mM. The results of cultivation of D. antarctica plants in the presence of cadmium ions for 63 days allow to determine the concentration range that does not inhibit the growth of the plants in vitro, and it was up to 1 mM. It was found that toxicant concentrations of 0.1 and 0.2 mM did not cause changes in the profiles of PCR products. After growing the plants with 0.2–1 mM CdCl2 for 17 days, the changes in the profiles of PCR products, indicating the mutagenic impact, were observed at concentrations of 0.6 mM or above; moreover, the number of changes increased in dependence on the concentration of heavy metal. Prolonged influence (140-265 days) of cadmium ions in relatively low concentrations (0.1 mM and 0.4 mM) did not cause detectable mutations. Conclusions. D. antarctica, a plant extremophile, which has evolved mechanisms of resistance to a variety of extreme conditions as a result of adaptation to the existence in the harsh conditions of Antarctica, shows enhanced resistance to cadmium ions in comparison with other species of vascular plants. Inhibition of growth occurs at Cd2+ concentrations of 0.1 mM or above, whereas concentrations of 1 mM or above cause cessation of growth and death of plants. Mutagenic effect on D. antarctica was observed at Cd2+ concentrations of above 0.4 mM. After prolonged growth of plants (for 3–8 months) at cadmium ions concentrations of 0.1–0.4 mM, genetic changes was not found.Keywords: Deschampsia antarctica Desv., plants in vitro obtained by microclonal propagation, cadmium ions, mutagenic effect, PCR-analysis.

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“…Mazhoudi et al (1997), Mocquot et al (1996) with control which can be attributed to the fact that much metal was not accumulated by plants and remained in rhizospheric zone so toxic effects didn't reach the thresholds to make any apparent DNA damage. The number of band appeared or disappeared in samples exposed to metals suggests different metals probably cause alterations at genomic level and therefore samples exposed to heavy metal stress would act as a marker for assessment of environmental exposition on metals Gjorgieva et al (2013). Plant mechanism effecting the root uptake and shoot transport of Cd can also affect the expression of Cd toxicity in plants Cakmak, (2000); Dunbar et al (2003).…”

Section: Microorganismsmentioning

confidence: 99%

Biochemical and RAPD Analysis ofHibiscus rosa sinensisInduced by Heavy Metals

Bhaduri

Fulekar

2014

Soil and Sediment Contamination: An International Journal

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Biochemical and RAPD analysis of Hibiscus rosa sinensis to assess the effects induced by heavy metals. Abstract:The present study was aimed to assess the effects of three different metals (Cadmium, Lead and Zinc) at same concentration on Hibiscus rosa sinensis during metal uptake. The effects of different metals at same concentrations were assessed on biomass, root-shoot length and biochemical parameters like chlorophyll and antioxidant enzymes like SOD and CAT to establish the tolerance potential and toxic effects on plants in different metals. The accumulation of metals by plants were found to be in the following order Zn > Mixed metals > Cd > Pb, where Zn was accumulated approximately 79.6% in plants tissues. Plants removed from Cd showed more enzyme activities than other two metals. DNA stability was investigated by Random Amplified Polymorphic DNA (RAPD) technique which demonstrated that the samples in Cd and mixed metal showed similar trend to each other, whereas samples in Zn and Pb showed similar band intensity with control. Resultssuggested that Cd and/or heavy metal stress influences antioxidant status and also induces DNA changes during remediation. Therefore these studies could be a useful biomarker assay for better treatment for metals remediation from soil by means of phytoremediation.

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Assessment of the genotoxicity of heavy metals inPhaseolus vulgaris L. as a model plant system by Random Amplified Polymorphic DNA (RAPD) analysis

Cited by 12 publications

References 32 publications

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms

Enhanced tolerance of Deschampsia antarctica Desv. to the mutagenic effect of cadmium ions

Biochemical and RAPD Analysis ofHibiscus rosa sinensisInduced by Heavy Metals

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