Porous scaffolds can be made out of silkworm and spider silk for cartilage regeneration. Mechanical properties are related to porosity and pore size of the construct. Cell spreading and cell expression depended on the porosity and pore-size.
Spider egg sac silk (SpESS) were enzymatically cleaned and their biodegradation in vivo and in vitro, as well as their biocompatibility were studied. Proteinase K treatment diminished the tenacity and the strain of the SpESS fibers in proportion to the enzyme concentration. Fibers treated with trypsin were not significantly affected. Tensile properties of Vicryl, SpESS and of silkworm (Bombyx mori) silk fibers (SWS) were measured after incubation in phosphate buffered saline (PBS) at 37 degrees C up to 12 weeks. Biodegradation of SpESS and SWS was insignificant compared to Vicryl. Five milligram SpESS fibers from laboratory grown spiders (Araneus diadematus) were treated with proteinases before sterilization and subcutaneously implanted in Wistar rats. After 1, 4 and 7 weeks the immunological reaction was compared to untreated SpESS and polyglactin (Vicryl) control samples. SpESS samples treated with trypsin only or in combination with a Proteinase K treatment induced less inflammatory reactions than untreated silk fibers. The enzymatical cleaning could diminish the tensile properties, but enhanced the biocompatibility of the SpESS fibers rendering them appropriate for use in biomaterial application where the slow biodegradability is an advantage.
Spider silk has attracted the attention of many scientists because of its desirable physical properties. Most of this attention has been devoted to dragline silk, a thread that has high tensile strength, high strain and ultra-low weight. To help understand structure-property relationships in spider silks, the tensile behavior of egg sac (cylindrical gland) silk of Araneus diadematus Clerck 1757 was compared with dragline (major ampullate gland) and silkworm silks. In addition, stress-strain curves of egg sac silk were simulated by a spring-dashpot model, specifically a Standard Linear Solid (SLS) model. The SLS model consists of a spring in series with a dashpot and in parallel with another spring, resulting in three unknown parameters. The average stress-strain curve of fibers from five different egg sacs could be accurately described by the model. Closer examination of the individual stress-strain curves revealed that in each egg sac two populations of fibers could be distinguished based on the parameters of the SLS model. The stress-strain curves of the two populations clearly differed in their behavior beyond the yield point and were probably derived from two different layers within the egg sac. This indicates that silks in the two layers of A. diadematus egg sacs probably have different tensile behavior.
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