Silkworm and spider silk scaffolds for chondrocyte support

Gellynck, Kris; Verdonk, Peter; Nimmen, Els Van; Almqvist, Karl; Gheysens, Tom; Schoukens, Gustaaf; Langenhove, Lieva Van; Kiekens, Paul; Mertens, Johan; Verbruggen, Gust

doi:10.1007/s10856-008-3474-6

Cited by 88 publications

(72 citation statements)

References 36 publications

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“…Rapid degradation [170] [171]; [172]; [173]; [174] Alginate Aids re-differentiation of de-differentiated chondrocytes [66] In vivo injectable options [175] Abundant and low cost Certain cross-linkage can result in poor biocompatibility [184]; [185]; [186]; [187]; [188]; [189]; [182]; [190] Dermatan sulphate, [194]; [195] …”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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The treatment of cartilage defects poses a clinical challenge owing to the lack of intrinsic regenerative capacity of cartilage. The use of tissue engineering techniques to bioengineer articular cartilage is promising and may hold the key to the successful regeneration of cartilage tissue. Natural and synthetic biomaterials have been used to recreate the microarchitecture of articular cartilage through multilayered biomimetic scaffolds. Acellular scaffolds preserve the microarchitecture of articular cartilage through a process of decellularization of biological tissue. Although promising, this technique often results in poor biomechanical strength of the graft. However, biomechanical strength could be improved if biomaterials could be incorporated back into the decellularized tissue to overcome this limitation.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Bioengineering of Articular Cartilage: Past, Present and Future

Felimban

Moulton

et al. 2013

Regen. Med.

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“…Este biopolímero extraído de gusanos de seda y arañas, se destaca por su biocompatibilidad, biodegradabilidad y excelentes propiedades mecánicas (Altman et al, 2003). Condrocitos aislados de cartílago articular humano y sembrados en soportes hechos con seda fueron capaces de adherirse, proliferar y secretar componentes de matriz extracelular del cartílago (Gellynck et al, 2008); lo mismo ha sido descrito al sembrar célu-las madre mesenquimales (CMM). Los inconvenientes de emplear soportes de seda están relacionados con el hecho de que se observan diferencias en la morfología de los condrocitos, la estructura zonal del tejido obtenido y la concentración celular necesaria para obtener el tejido cartilaginoso (Wang et al, 2006).…”

Section: Productounclassified

Terapias Celulares y Productos de Ingeniería de Tejidos para el Tratamiento de Lesiones Condrales de Rodilla

Cabrera

Duque²,

Fontanilla

2017

Rev. colomb. biotecnol.

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RESUMENEl cartílago articular es un tejido vulnerable a las lesiones de diferente etiología; siendo uno de los más afectados, el cartílago de la rodilla. Aunque la mayoría de los tratamientos convencionales reducen los síntomas, generalmente conducen a la formación de fibrocartílago; el cual, posee características diferentes a las del cartílago hialino de las articulaciones. Son pocas las aproximaciones terapéuticas que promueven el reemplazo del tejido dañado por cartílago hialino funcional; las más exitosas son las denominadas terapias avanzadas, que aplican células y productos de ingeniería de tejidos con el fin de estimular la regeneración del cartílago. La mayoría de ellas se basan en colocar soportes hechos con biomateriales de diferente origen, que sembrados o no con células exógenas o endógenas, reemplazan al cartílago dañado y promueven su regeneración. Este trabajo revisa algunas de las aproximaciones terapéuticas enfocadas en la regeneración del cartílago articular de rodilla; así como, los biomateriales más empleados en la elaboración de soportes para terapia celular e ingeniería de tejido cartilaginoso.Palabras clave: biomaterial, cartílago, lesiones de rodilla, ingeniería de tejidos. ABSTRACTThe articular cartilage is prone to suffer lesions of different etiology, being the articular cartilage lesions of the knee the most common. Although most conventional treatments reduce symptoms they lead to the production of fibrocartilage, which has different characteristics than the hyaline cartilage of the joint. There are few therapeutic approaches that promote the replacement of damaged tissue by functional hyaline cartilage. Among them are the so-called advanced therapies, which use cells and tissue engineering products to promote cartilage regeneration. Most of them are based on scaffolds made of different biomaterials, which seeded or not with endogenous or exogenous cells, can be used as cartilage artificial replacement to improve joint function. This paper reviews some therapeutic approaches focused on the regeneration of articular cartilage of the knee and the biomaterials used to develop scaffolds for cell therapy and tissue engineering of cartilage. ARTÍCULO DE REVISIÓNRev.

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“…Natural dragline silk fibers have also been used to culture human primary Schwann cells and demonstrate these cells can adhere and elongate (Allmeling et al, 2006). In vivo biocompatibility of native dragline silks has also been demonstrated by subcutaneous implantation in pigs , mice and rats (Gellynck et al, 2008). These studies have revealed that spider silk is well tolerated and generates little, if any, inflammatory response in the host animal.…”

Section: Other Applicationsmentioning

confidence: 99%

“…In addition to spinning silk threads for a host of applications other uses for spider silk proteins are being pursued, including drug delivery and scaffolding materials. Both dragline silk fibers and egg sacs have been studied for their biocompatibility in a few human cell culture systems (Allmeling et al, 2006;Gellynck et al, 2008). Primary chondrocytes have been shown to attach and survive for several weeks on natural dragline silk and egg sacs (Gellynck et al, 2008).…”

Section: Other Applicationsmentioning

confidence: 99%

“…Both dragline silk fibers and egg sacs have been studied for their biocompatibility in a few human cell culture systems (Allmeling et al, 2006;Gellynck et al, 2008). Primary chondrocytes have been shown to attach and survive for several weeks on natural dragline silk and egg sacs (Gellynck et al, 2008). Natural dragline silk fibers have also been used to culture human primary Schwann cells and demonstrate these cells can adhere and elongate (Allmeling et al, 2006).…”

Section: Other Applicationsmentioning

confidence: 99%

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