Ascorbate oxidase, prepared by a procedure which employs DEAE-cellulose chromatography and starch-column electrophoresis, has been obtained in high purity and in relatively high yield from either yellow or green summer squash. The enzyme had a specific
Small pieces of melanoma tissue were obtained from swordtail-platyfish hybrids and grafted into the embryos of swordtails, platyfish and hybrid embryos.
Several types of cells appeared in all the grafts: melanophages, two kinds of melanocytes, and macromelanophores were observed and described.
It was found that the environment of the grafted tissue played a predominant role in the survival of the graft. Grafts to swordtail embryos survived only a short time, whereas grafts to platyfish embryos bearing the gene for macromelanophores survived significantly longer. It was found that hybridization, with or without the macromelanophore gene, presented a still more favourable environment for the growth and migration of pigmented cells.
In no case was it possible to establish and maintain a permanent strain of tumour cells by embryonic transplantation.
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