Context:Irrational use of antibacterial drugs in Community Health-Care Centers (CHCs) may lead to increased resistance, morbidity, and mortality.Aims:The aim of this study was to determine patterns of antibacterial use at CHCs in a district of Indonesia and use this as data for an antibiotic policy.Settings and Design:The observational-descriptive study was conducted in a district of Indonesia to obtain antibacterial use from 2008 to 2010.Subjects and Methods:The data obtained from the report on the use of medicines were classified and processed using the anatomical therapeutic chemical (ATC) and defined daily doses (DDD) method, with DDD/1000 patients as a unit measurement. The number of patients was obtained from attending patients in that research period. The most abundant antibacterial drugs use segment was identified by the drug utilization 90% (DU90%) method.Statistical Analysis Used:Descriptive analysis were performed in this study.Results:Fourteen kinds of antibacterial drugs were used in 61 CHCs. The total of antibacterial drug use during the period 2008–2010 was 871.36 DDD/1000 patients/day. Declining antibacterial use was observed between 2008 and 2010. Six kinds of antibacterial drugs were the most commonly used. The data show that the average use per visit was as high as 24.41 DDD.Conclusions:Amoxicillin, sulfamethoxazole and trimethoprim are antibacterials that have to be reconsidered by physicians for use in the Bandung CHC. The high use of antibacterial drugs, as described in the study, can be used as reference to develop an antimicrobial stewardship program and increase awareness of resistance, adverse drug reaction and drug interaction of antibacterial drugs.
Melanocytic tumors are a type of cancer that is most commonly found on the skin. The melanoma prevalence rate has risen dramatically over the last 50 years. As a result, the discovery of new therapeutic agents is critical. The BRAF kinase is one of the receptors involved in cell apoptosis. Dabrafenib is a selective BRAF inhibitor with common side effects such as rash, photosensitivity, and hyperkeratosis. Meanwhile, Acalypha indica is a plant that has been widely reported as a source of antiproliferative and proapoptotic compounds. However, the phytochemicals in A. indica that play an important role in melanocytic tumors have yet to be discovered. Molecular docking is a structure-based drug design method that is used to identify potential hits during the drug discovery process. The aims of this study are to obtain candidate lead compounds for BRAF kinase based on binding mode interaction and binding stability by using AutoDock 4.2 and GROMACS 2019.6, respectively, for molecular docking and molecular dynamics (MD). The native ligand, SM5, has estimated free energy of binding and an inhibitory constant of -5.93 kcal/mol and 45.30 µM, respectively. 2-Methyl anthraquinone, chrysin, stigmasterol, and γ-sitosterol have higher binding energy, with an estimated free energy of binding of -6.24, -6.67, -6.35, and -6.14 kcal/mol, respectively. According to the MD simulation, stigmasterol and γ-sitosterol will be more effective at stabilizing the 6XFP complex during 100 ns. Finally, stigmasterol and γ-sitosterol are potential lead compounds as BRAF inhibitors.
Objective: This study investigated the structure-based molecular interactions between phytochemical constituents of Acalypha indica L. and caspase-3.
Methods: Thirty-three phytochemical constituents of A. indica were screened against caspase-3. The X-ray crystal structure of human caspase-3 was retrieved from https://www.rcsb.org/structure/. The molecular interactions of the phytochemicals were studied using the AutoDock 4.2 software and followed by molecular dynamics (MD) simulations using the Amber18 software.
Results: From this study, 25 screened phytochemicals were found to have a better binding mode than the native ligand. Moreover, the binding stability of the top four hits evaluated by MD indicated that the hydrogen bonds in MD were quite different from the molecular docking results due to the massive receptor and ligand movement in the MD simulations. However, with the exception of stigmasterol, all ligands were able to stabilize the protein.
Conclusion: This study suggested that γ-sitosterol acetate, β-sitosterol acetate, and γ-sitosterol might be able to induce caspase-3, thereby activating apoptosis. These high-affinity compounds can bind to caspase-3 more efficiently and were able to stabilize the protein. Therefore, they have the potential to be used as lead compounds in the treatment of cancer.
POTENTIAL DEVELOPMENT OF WOMAN FARMING GROUP IN THE UTILIZATION OF THE YARD FOR DRUG AND COSMETICS PLANTS USING VERTICAL GARDEN METHOD IN CIBIRU WETAN VILLAGE, BANDUNG REGENCY. The village of Cibiru Wetan is largely a rice field and swidden area with hilly contours and insufficient water availability. One of the utilization of fields with a lack of water is by growing plants that require minimal water, including medicinal plants and cosmetics. The initiation of this activity has a high prospect of success due to the high initiative and participation of the population in the form of women's farmer groups and the family welfare team as evidenced by the many achievements, awards, and skills gained by the Cibiru Wetan Village through the participation of its citizens. Therefore, the purpose of the project activity was socialization and practice at women’s farmer group of Cibiru Wetan Village through the use of medicinal plants and cosmetics with simple appropriate technology through vertical gardening. The method of implementation used in the training program for planting medicinal plants was divided two stage, that was providing information about family medicinal plants ranging from understanding to the benefits of the plants, and the last step was explaining or training and direct practice of planting medicinal plants and cosmetics using vertical planting. The results showed a positive understanding of the two groups of women’s farmer group I and II and the family welfare team, both for the stage of socialization and counseling as well as for the practice phase of planting medicinal plants and cosmetics vertically. The conclusion was the use of medicinal plants and cosmetics could increase the understanding of the target group and the compost produced by residents could be utilized through this activity.
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