The uptake of polyelectrolyte multilayer coated colloids into cells, subsequent defoliation and plasmid delivery was studied by means of confocal microscopy and flow cytometry. Silica particles coated layer-wise with protamine and dextran sulfate were given to HEK 293T cells. Optimum uptake was found with protamine as the top layer. The particle uptake likely follows an non-receptor-mediated endocytotic pathway. Defoliation of polyelectrolyte multilayer coated particles within cells was demonstrated by the release of incorporated plasmids as indicated by the expression of plasmid encoded proteins using the enhanced green fluorescence proteine (pEGFP-C1) plasmid and a red fluorescence protein (pDsRed1-N1) plasmid. This proves, together with the direct observation of fluorescent layer debris, the defoliation of coated particles and the release of layer components into the cytoplasm. Particle uptake and GFP expression.
Cover: Silica particles can be coated layerwise with polyelectrolytes. Using the plasmid encoding the green fluorescent protein (pEGFP-C1) as a layer constituent particle uptake, release of particles into cytoplasm and defoliation of the polyelectrolyte multilayer are demonstrated by GFP expression in HEK 293T cells. Further details can be found in the Full Paper by U. Reibetanz, C. Claus, E. Typlt, J. Hofmann, and E. Donath* on page 153.
Biocomposite thin films assembled on colloidal particles by means of layer-by-layer adsorption have been suggested as drug carriers and diagnostic devices. Protamine (PRM)/dextransulfate (DXS) and protamine/bovine serum albumine (BSA) multilayers were fabricated on colloidal silica and subsequently investigated by means of fluorescence activated cell sorting (FACS) and microelectrophoresis. Fluorescein labeled polyelectrolytes were embedded at different positions in the multilayers as a marker for layer growth. FACS showed that PRM and DXS formed regular growing stable multilayers, yet adsorbed PRM can be nevertheless exchanged with PRM in solution during layer formation and also after the multilayer formation has been completed. Up to 90% of the PRM pool was available for exchange. PRM together with BSA as demonstrated by SFM did not form multilayers under the applied conditions although the zeta-potential, commonly used as an indicator for stepwise adsorption, observed characteristic alternations. The capability of bound PRM to exchange with PRM in solution is attributed to its relatively small size. The demonstrated exchange may have importance in designing multilayers with smart release features. Furthermore, FACS proved to be a rather suitable means to quantify the aggregation behavior during coating and washing. Singulets, doublets, triplets, and aggregates of higher order could be clearly resolved. The aggregation of particles coated with PRM/DXS layers was higher than that of silica particles coated with PAH/PSS layers. In the first case about 50% of all recorded events are attributed to aggregats, while the PAH/PSS coating produced only about 10% aggregates.
Polyelectrolyte capsules were fabricated by layer-by-layer deposition of poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) on glutardialdehyde fixed human erythrocytes and subsequent core dissolution using NaOCl as an oxidizing agent. SANS together with confocal laser scanning microscopy (CLSM) were applied to study capsule topology and interior as well as the layer thickness as a function of the deposition protocol, layer number, ionic concentration, and temperature treatment. The capsules contained various amounts of trapped polyelectrolyte. Retention depended on the order of polyelectrolyte deposition and layer number, which influenced layer permeability. The capsule wall thickness was found to be much smaller (3-4.5 nm in total) than what was known for polyelectrolyte multilayer walls, where every single layer contributes about 1.8 nm to the total thickness. NaCl (0.1 mM) caused a layer thickness decrease by 16%. Annealing at 70 degrees C induced capsule shrinking together with an increase of the wall thickness by 85% and wall density by 12%.
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