The aim of this research was to determine the sun protection factor (SPF) of sunscreens emulsions containing chemical and physical sunscreens by ultraviolet spectrophotometry. Ten different commercially available samples of sunscreen emulsions of various manufactures were evaluated. The SPF labeled values were in the range of 8 to 30. The SPF values of the 30% of the analyzed samples are in close agreement with the labeled SPF, 30% presented SPF values above the labeled amount and 40% presented SPF values under the labeled amount. The proposed spectrophotometric method is simple and rapid for the in vitro determination of SPF values of sunscreens emulsions.

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The aim of this research was the development and validation of a high performance liquid chromatography (HPLC) method for the simultaneous and quantitative determination of benzophenone-3, octyl methoxycinnamate and octyl salicylate contained in sunscreen emulsions. The separation and quantitative determination was achieved using a LiChrospher((R)) 100 RP-18 (5 microm) Merck column, a mobile phase constituted of methanol/water (85/15, v/v), a flow-rate of 1.0 mL min(-1) and UV detection at 310 nm. The correlation coefficients and percentage of recovery for benzophenone-3, octyl methoxycinnamate and octyl salicylate were 0.9999 and 99.46%; 0.9995 and 98.85%; 0.9998 and 98.84%, respectively. The relative standard deviations (RSD) for commercial samples were between 0.50 and 0.70%.

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